JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB313966

Human GNMT ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human GNMT ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human GNMT in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Glycine N-methyltransferase, GNMT

1 Images
Sandwich ELISA - Human GNMT ELISA Kit (AB313966)
  • sELISA

Supplier Data

Sandwich ELISA - Human GNMT ELISA Kit (AB313966)

Example of standard curve using ab313966. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.5 ng/mL

Range

2.5 - 500 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human GNMT ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GNMT in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human GNMT coated on a 96-well plate.
style
left-column

Recovery

[ { "sample": "Serum", "range": "91 - 126 %", "average": "= 104.5" }, { "sample": "Plasma", "range": "75 - 107 %", "average": "= 95.91" }, { "sample": "Cell culture media", "range": "81 - 133 %", "average": "= 122.8" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313966-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313966-CMP04" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313966-CMP07" }, { "size":"1 x 1 Unit", "name":"Human GNMT Antibody-coated ELISA Plate", "number":"AB313966-CMP09" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB313966-CMP03" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313966-CMP08" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313966-CMP05" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human GNMT Protein Standard", "number":"AB313966-CMP11" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB313966-CMP06" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human GNMT Detection Antibody", "number":"AB313966-CMP10" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313966-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313966-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"ELISA Microplate Bag", "number":"AB313966-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-Well Plate Sealing Film", "number":"AB313966-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB313966-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313966-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB313966-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313966-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313966-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human GNMT Antibody-coated ELISA Plate", "number":"AB313966-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human GNMT Detection Antibody", "number":"AB313966-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human GNMT Protein Standard", "number":"AB313966-CMP11", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNMT also known as Glycine N-methyltransferase functions by catalyzing the methyl transfer from S-adenosylmethionine (SAM) to glycine which results in the formation of N-methylglycine. This enzyme possesses a molecular mass of approximately 292 amino acids. GNMT expresses strongly in the liver but also occurs in other tissues like the pancreas. It maintains the balance of SAM and influences cellular methylation processes.
Biological function summary

GNMT plays a vital role in regulating the cellular methyl group pool. It lacks association with any complex; instead GNMT works independently to control the levels of SAM influencing numerous methylation reactions. Its activity helps in maintaining normal liver function and detoxification processes within the body. Furthermore GNMT impacts the metabolism of several amino acids.

Pathways

GNMT participates in the methionine cycle and folate metabolism pathways. In the methionine cycle GNMT regulates SAM levels alongside proteins such as methionine adenosyltransferase and S-adenosylhomocysteine hydrolase which influence methylation activities broadly. Through its action within the folate metabolism it connects indirectly with proteins like dihydrofolate reductase contributing to nucleic acid synthesis and cellular growth.

GNMT shows a significant association with liver-related diseases and certain cancers. Altered GNMT expression or activity links with liver dysfunction and hepatocellular carcinoma. Additionally in cancer GNMT dysregulation interacts with proteins like tumor suppressor p53 influencing cell proliferation and apoptosis. GNMT's effect on SAM levels affects global DNA methylation impacting gene expression and contributing to disease progression.
style
left-column

Product protocols

style
left-column

Target data

Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy), a reaction regulated by the binding of 5-methyltetrahydrofolate. Plays an important role in the regulation of methyl group metabolism by regulating the ratio between S-adenosyl-L-methionine and S-adenosyl-L-homocysteine.
See full target information Glycine N-methyltransferase
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Primary Antibodies

AB224753

Anti-GNMT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNMT antibody (AB224753)

  • 0
  • 0
Proteins & Peptides

AB79190

Recombinant Human GNMT protein

SDS-PAGE - Recombinant Human GNMT protein (AB79190)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com