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AB309418

Human GPR56 ELISA Kit

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Human GPR56 ELISA Kit is a Sandwich ELISA for the measurement of Human GPR56 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

GPR56, TM7LN4, TM7XN1, UNQ540/PRO1083, ADGRG1, Adhesion G-protein coupled receptor G1, G-protein coupled receptor 56

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Sandwich ELISA - Human GPR56 ELISA Kit (AB309418)
  • sELISA

Supplier Data

Sandwich ELISA - Human GPR56 ELISA Kit (AB309418)

Example of standard curve using ab309418. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 16 pg/mL

Range

16 - 4000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB302910

Anti-GPR56 antibody [EPR26546-78] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Anti-GPR56 antibody [EPR26546-78] - BSA and Azide free (AB302910)

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  • 0
Cell Lines & Lysates

AB288698

Human ADGRG1 knockout HEK-293 cell line

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human GPR56 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD42b in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human GPR56 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "72 - 110 %", "average": "= 90.51" }, { "sample": "Plasma", "range": "67 - 90 %", "average": "= 78.75" }, { "sample": "Cell culture supernatant", "range": "79 - 109 %", "average": "= 93.42" }, { "sample": "Cell culture media", "range": "79 - 109 %", "average": "= 93.42" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309418-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309418-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human GPR56 Detection Antibody", "number":"AB309418-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309418-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human GPR56 Antibody-coated ELISA Plate", "number":"AB309418-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB309418-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309418-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human GPR56 Protein Standard", "number":"AB309418-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309418-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPR56 also known as ADGRG1 is a G protein-coupled receptor. It has a mass of around 75 kDa. It plays an essential role in cellular signaling. Found in various tissues GPR56 is expressed significantly in brain and prostate tissue. This receptor is part of the adhesion GPCR family characterized by its large N-terminal region which is involved in cell adhesion and interaction. The mechanical functions of GPR56 involve regulating cell communication which influences cellular growth and behavior.
Biological function summary

GPR56 participates in brain development particularly in the formation of the cerebral cortex. It interacts with extracellular matrix components like collagen III which impacts cell adhesion and migration processes. GPR56 does not commonly form complexes but interacts with other cell membrane molecules to carry out its biological roles. Research indicates its involvement in cellular processes such as cell signaling pathways and the maintenance of tissue integrity.

Pathways

GPR56 is involved in both the inside-out and outside-in signaling pathways. In these pathways GPR56 modulates cell adhesion and migration dynamics. Related proteins such as collagen III interact with GPR56 to facilitate these processes. Furthermore the receptor interacts with integrin signaling pathways that help regulate the homeostasis of the extracellular matrix proving critical in maintaining normal cellular physiology.

Mutations or dysregulation of GPR56 have been associated with brain malformations such as bilateral frontoparietal polymicrogyria. It also plays a role in certain cancers including melanoma where its function in cell adhesion contributes to metastasis. Researchers study its relationship with proteins like integrins which when altered may contribute to cancer progression. Understanding GPR56's roles could offer new insights into potential therapeutic targets for these conditions.
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Product protocols

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Target data

Adhesion G-protein coupled receptor (aGPCR) for steroid hormone 17alpha-hydroxypregnenolone (17-OH), which is involved in cell adhesion and cell-cell interactions (PubMed : 39389061). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as RhoA pathway (PubMed : 28874577, PubMed : 35418682, PubMed : 39389061). ADGRG1 is coupled to G(12) and/or G(13) G proteins (GNA12 and GNA13, respectively) and mediates the activation Rho small GTPases (PubMed : 22238662, PubMed : 28424266, PubMed : 35418682, PubMed : 39389061). Acts as a potent suppressor of ferroptosis : binding to 17-OH-binding initiates signaling that down-regulates CD36 and alleviates ferroptosis-induced liver injury (By similarity). Ligand-binding also induces cell adhesion activity via association with proteins such as collagen III/COL3A1 and TGM2 (By similarity). Mediates cell matrix adhesion in developing neurons and hematopoietic stem cells (By similarity). Involved in cortical development, specifically in maintenance of the pial basement membrane integrity and in cortical lamination : association with COL3A1 in the developing brain inhibits neuronal migration via activation of the RhoA pathway (PubMed : 24531968). Together with TGM2, acts as a regulator of myelination and myelin repair in oligodendrocyte precursor cells (By similarity). Acts as a hemostatic sensor of shear force : G protein-coupled receptor signaling is activated in response to shear force in platelets, promoting G(13) G protein signaling, and platelet shape change and aggregation in a COL3A1-dependent manner (PubMed : 33097663). Acts as an inhibitor of VEGFA production thereby inhibiting angiogenesis through a signaling pathway mediated by PRKCA (PubMed : 16757564, PubMed : 19572147, PubMed : 21724588). Plays a role in the maintenance of hematopoietic stem cells in bone marrow niche (By similarity). Plays an essential role in testis development (By similarity).
See full target information ADGRG1
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Product promise

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