Human GPR56 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human GPR56 in Human in Plasma, Serum, Cell culture supernatant samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 16 - 4000 pg/mL
- Sensitivity
- = 16 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor involved in cell adhesion and probably in cell-cell interactions. Mediates cell matrix adhesion in developing neurons and hematopoietic stem cells. Receptor for collagen III/COL3A1 in the developing brain and involved in regulation of cortical development, specifically in maintenance of the pial basement membrane integrity and in cortical lamination (By similarity). Binding to the COL3A1 ligand inhibits neuronal migration and activates the RhoA pathway by coupling to GNA13 and possibly GNA12 (PubMed:22238662). Plays a role in the maintenance of hematopoietic stem cells and/or leukemia stem cells in bone marrow niche (By similarity). Plays a critical role in cancer progression by inhibiting VEGFA production threreby inhibiting angiogenesis through a signaling pathway mediated by PRKCA (PubMed:16757564, PubMed:21724588). Plays an essential role in testis development (By similarity). ADGRG1 N-terminal fragment. Plays a critical role in cancer progression by activating VEGFA production and angiogenesis through a signaling pathway mediated by PRKCA (PubMed:21724588).
Alternative names
GPR56, TM7LN4, TM7XN1, UNQ540/PRO1083, ADGRG1, Adhesion G-protein coupled receptor G1, G-protein coupled receptor 56, Protein TM7XN1
What's included?
Recommended products
Human GPR56 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human GPR56 in Human in Plasma, Serum, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 16 - 4000 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 16 pg/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 90.51 | Range 72 - 110 % |
Sample type Plasma | Average % = 78.75 | Range 67 - 90 % |
Sample type Cell culture supernatant | Average % = 93.42 | Range 79 - 109 % |
Sample type Cell culture media | Average % = 93.42 | Range 79 - 109 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human GPR56 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD42b in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human GPR56 coated on a 96-well plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GPR56 also known as ADGRG1 is a G protein-coupled receptor. It has a mass of around 75 kDa. It plays an essential role in cellular signaling. Found in various tissues GPR56 is expressed significantly in brain and prostate tissue. This receptor is part of the adhesion GPCR family characterized by its large N-terminal region which is involved in cell adhesion and interaction. The mechanical functions of GPR56 involve regulating cell communication which influences cellular growth and behavior.
- Biological function summary
GPR56 participates in brain development particularly in the formation of the cerebral cortex. It interacts with extracellular matrix components like collagen III which impacts cell adhesion and migration processes. GPR56 does not commonly form complexes but interacts with other cell membrane molecules to carry out its biological roles. Research indicates its involvement in cellular processes such as cell signaling pathways and the maintenance of tissue integrity.
- Pathways
GPR56 is involved in both the inside-out and outside-in signaling pathways. In these pathways GPR56 modulates cell adhesion and migration dynamics. Related proteins such as collagen III interact with GPR56 to facilitate these processes. Furthermore the receptor interacts with integrin signaling pathways that help regulate the homeostasis of the extracellular matrix proving critical in maintaining normal cellular physiology.
- Associated diseases and disorders
Mutations or dysregulation of GPR56 have been associated with brain malformations such as bilateral frontoparietal polymicrogyria. It also plays a role in certain cancers including melanoma where its function in cell adhesion contributes to metastasis. Researchers study its relationship with proteins like integrins which when altered may contribute to cancer progression. Understanding GPR56's roles could offer new insights into potential therapeutic targets for these conditions.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Sandwich ELISA - Human GPR56 ELISA Kit (ab309418)
Example of standard curve using ab309418. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com