AB315044

Human GPVI ELISA Kit

SimpleStep
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Human GPVI ELISA Kit is a single-wash 90-min Simplestep used to quantify Human GPVI with a sensitivity of 1.711 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Platelet glycoprotein VI, GPVI, Glycoprotein 6, GP6

3 Images

Sandwich ELISA - Human GPVI ELISA Kit (AB315044)

sELISA

Supplier Data

Sandwich ELISA - Human GPVI ELISA Kit (AB315044)

Interpolated concentration of native GPVI was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 100, plasma (EDTA) 1 : 40, plasma (citrate) 1 : 40, and plasma (heparin) 1 : 40. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human GPVI ELISA Kit (AB315044)

Example of human GPVI standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human GPVI ELISA Kit (AB315044)

Interpolated concentration of native GPVI was measured in duplicate at different sample concentrations. Undiluted samples are as follows : platelet poor plasma 1 : 32 and a matched plasma (EDTA) control 1 : 50. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, Heparin Plasma, EDTA Plasma, Citrate plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.711 pg/mL

Range

5.625 - 360 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human GPVI SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GPVI protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma.Quantitate Human GPVI with 1.711 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]

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Recovery

[ { "sample": "Serum", "range": "105 - 115 %", "average": "= 109" }, { "sample": "Heparin Plasma", "range": "100 - 115 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "99 - 120 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "102 - 111 %", "average": "= 107" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPVI or glycoprotein VI functions mechanically as a collagen receptor on the surface of platelets. With a molecular mass around 62 kDa it is expressed mainly on megakaryocytes and platelets. GPVI plays an important role in platelet adhesion and activation by binding to collagen exposed at sites of vascular injury. This interaction initiates a cascade of platelet activation essential for normal hemostasis.

Biological function summary

The target participates in the formation of platelet activation complexes. GPVI partners with the Fc receptor γ-chain to transmit activation signals upon collagen binding. This process results in platelet shape change granule secretion and aggregation which are all critical steps for clot formation. By interacting with collagen GPVI ensures that platelets can effectively respond to vascular damage particularly in arterial vessels where high shear forces prevail.

Pathways

The glycoprotein VI contributes significantly to the immune and coagulation pathways. It activates the enzyme Syk kinase following collagen binding which further triggers the downstream signaling pathways like the MAPK pathway leading to platelet activation. GPVI works closely with other receptors like integrin α2β1 and the glycoprotein Ib/V/IX complex reinforcing platelet-collagen interactions and stabilizing the platelet plug formation during thrombus growth.

Glycoprotein VI shows relevance in cardiovascular diseases and immune thrombocytopenia. Abnormal GPVI function or expression can exacerbate conditions such as myocardial infarction by promoting excessive platelet activation and thrombosis. Conversely reduced GPVI levels relate to bleeding disorders due to impaired platelet function. Studies have also linked GPVI to autoimmune conditions where antibodies may target it similar to responses in immune thrombocytopenia.

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Product protocols

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Target data

Collagen receptor involved in collagen-induced platelet adhesion and activation. Plays a key role in platelet procoagulant activity and subsequent thrombin and fibrin formation. This procoagulant function may contribute to arterial and venous thrombus formation. The signaling pathway involves the FcR gamma-chain, the Src kinases (likely FYN or LYN) and SYK, the adapter protein LAT and leads to the activation of PLCG2.

See full target information GP6

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human GPVI ELISA Kit

Sandwich ELISA - Human GPVI ELISA Kit (AB315044)

Sandwich ELISA - Human GPVI ELISA Kit (AB315044)

Sandwich ELISA - Human GPVI ELISA Kit (AB315044)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

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Primary Antibodies

Product promise