Human Granulysin ELISA Kit
- Recombinant
- SimpleStep
- What is this?
Be the first to review this product! Submit a review
|
(1 Publication)
Human Granulysin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Granulysin with a sensitivity of 8.22 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
LAG2, NKG5, TLA519, GNLY, Granulysin, Lymphokine LAG-2, Protein NKG5, T-cell activation protein 519
- sELISA
Supplier Data
Sandwich ELISA - Human Granulysin ELISA Kit (AB256402)
Interpolated concentrations of native Granulysin in human placenta extract based on a 250 μg/mL extract load.
The concentrations of Granulysin were measured in duplicate and interpolated from the Granulysin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granulysin concentration was determined to be 5888.16 pg/mL in placenta extract.
- sELISA
Supplier Data
Sandwich ELISA - Human Granulysin ELISA Kit (AB256402)
50% Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granulysin concentration was determined to be 2536.17 pg/mL with a range of 500.32 – 5863.75 pg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human Granulysin ELISA Kit (AB256402)
Example of human Granulysin standard curve in Sample Diluent NS.
The Granulysin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Granulysin ELISA Kit (AB256402)
Neat unstimulated and PHA-M stimulated PBMC cell culture were measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granulysin concentration was determined to be 274.19 pg/mL in unstimulated PBMC supernatant 3720.93 pg/mL in stimulated PBMC supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Human Granulysin ELISA Kit (AB256402)
Interpolated concentrations of native Granulysin in human serum, plasma and cell culture supernatant samples.
The concentrations of Granulysin were measured in duplicates, interpolated from the Granulysin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50% and stimulated PBMC cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granulysin concentration was determined to be 1153.29 pg/mL in serum, 790.67 pg/mL in plasma (citrate), 1668.29 pg/mL in plasma (EDTA), and 3387.74 pg/mL in stimulated PBMC supernatant.
Reactivity data
Product details
Human Granulysin ELISA Kit (ab256402) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Granulysin protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Granulysin with 8.22 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Granulysin (GNLY) is a 15.5 kD protein is a member of the saposin-like protein (SAPLIP) family. It is mapped to 2p11.2. Granulysin functions to create holes in the target cell membrane and destroy it. It is a microbiocidal via induction of apoptosis. The gene is expressed in cytolytic granules with perforin, a pore forming protein as well as granzymes that are also involved in cytolysis. In addition to it. As an antimicrobial, Granulysin’s activities include killing microbes that cause tuberculosis and malaria. It has been reported to be involved in tumor destruction. A series of peptides generated from the amino acid sequence of Granulysin are potential antibiotics. Granulysin shares 76% and 26% sequence homology with monkey and mouse, respectively.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Granulysin contributes to the body's defense against bacterial fungal and parasitic infections. It acts independently but can also collaborate with other immune components to enhance its effects. Granulysin disrupts microbial membranes owing to its affinity to negatively charged lipids leading to pathogen elimination. This mechanism plays a critical role in the human body's broader immune response.
Pathways
Granulysin operates significantly within the immune response pathway by working in concert with perforin another cytolytic protein. Together they form a potent immune mechanism aimed at eliminating infected or malignant cells. Furthermore the interferon-gamma pathway closely influences Granulysin suggesting a connection to innate and adaptive immune responses.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Journal of extracellular biology 3:e166 PubMed39022723
2024
Applications
Unspecified application
Species
Unspecified reactive species
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com