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AB46142

Human Granzyme B ELISA Kit (CTLA-1)

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(10 Publications)

Human Granzyme B ELISA Kit (CTLA-1) is a Sandwich ELISA for the measurement of Human Granzyme B (CTLA-1) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CGL1, CSPB, CTLA1, GRB, GZMB, Granzyme B, C11, CTLA-1, Cathepsin G-like 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, Granzyme-2, Human lymphocyte protein, SECT, T-cell serine protease 1-3E, CTSGL1, Lymphocyte protease, HLP

2 Images
Sandwich ELISA - Human Granzyme B ELISA Kit (CTLA-1) (AB46142)
  • sELISA

Lab

Sandwich ELISA - Human Granzyme B ELISA Kit (CTLA-1) (AB46142)

Hu Granzyme B (CTLA-1) measured in biologicals showing quantity (pg) per mL of tested sample

Sandwich ELISA - Human Granzyme B ELISA Kit (CTLA-1) (AB46142)
  • sELISA

Lab

Sandwich ELISA - Human Granzyme B ELISA Kit (CTLA-1) (AB46142)

Representative Standard Curve using ab46142

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 20 pg/mL

Range

31.25 - 1000 pg/mL

Assay time

3h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Granzyme B (CTLA-1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Granzyme B in Human serum, plasma, buffered solutions and cell culture medium.

A monoclonal antibody specific Granzyme B has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Granzyme B concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Granzyme B are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Granzyme B present in the samples.

This kit will recognize both endogenous and recombinant Human Granzyme B.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 8, "mean": "536 pg/mL", "standardDeviation": "30", "coefficientOfVariability": "5.59" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 8, "mean": "138 pg/mL", "standardDeviation": "9", "coefficientOfVariability": "6.76" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 15, "mean": "356 pg/mL", "standardDeviation": "18", "coefficientOfVariability": "5.06" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 15, "mean": "73 pg/mL", "standardDeviation": "5", "coefficientOfVariability": "7.38" } ]
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Recovery

[ { "sample": "Serum", "range": "67 - 78 %", "average": "= 73" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Granzyme B also known as GZMB GB11 or granzyme B protein is a serine protease with a molecular mass of approximately 32 kDa. It is expressed mainly in cytotoxic T lymphocytes and natural killer (NK) cells. This enzyme plays a mechanical role in inducing apoptosis in target cells serving as an effector protein in the immune system's defense against virally infected cells or transformed cancer cells. The activity of granzyme B relies on its ability to cleave after aspartate residues in substrate proteins leading to the activation of apoptotic pathways.
Biological function summary

Granzyme B participates prominently in the immune response by activating caspases particularly caspase-3 which promotes the breakdown of cellular components necessary for apoptosis. Granzyme B does not function in isolation but acts in concert with other immune system factors such as perforin to effectively induce cell death. Perforin creates pores in the target cell membrane allowing granzyme B to enter and instigate the apoptosis sequence. The enzyme also contributes to the processing of cytokines which enhances the immune response further.

Pathways

Studies have determined that granzyme B is critical in the apoptosis pathway particularly in the granule exocytosis pathway. It closely interacts with proteins such as perforin and other granzymes to mediate apoptosis in target cells. Granzyme B also plays a role in the inflammatory response and can influence pathways associated with cytotoxic T cell signaling. Its pathway interactions ensure effective elimination of damaged or infected cells maintaining tissue homeostasis.

Granzyme B has associations with autoimmune diseases and cancer. Abnormally high levels of granzyme B can contribute to tissue damage and inflammation in autoimmune conditions like rheumatoid arthritis. In the context of cancer granzyme B aids in tumor surveillance and destruction when functioning correctly but impaired granzyme B activity can lead to evasion of immune detection by cancerous cells. Perforin also plays a role in these conditions closely working with granzyme B to either protect against or drive disease progression.
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Product protocols

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Target data

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed : 1985927, PubMed : 3262682, PubMed : 3263427). It cleaves after Asp (PubMed : 1985927, PubMed : 8258716). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed : 31953257, PubMed : 32188940). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis (PubMed : 9852092). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (By similarity).
See full target information GZMB
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 56:e12997 PubMed38126537

2023

The level of Tim-3+CD8+ T cells can serve as a potential marker for evaluating the severity of acute graft-versus-host disease after haplo-PBSCT.

Applications

Unspecified application

Species

Unspecified reactive species

Nannan Pang,Mingkai Yu,Jianli Xu,Hailong Yuan,Gang Chen,Dong Wang,Chunxia Han,Weiguo Wang,Jianbing Ding,Ming Jiang

The Journal of dermatology 50:1190-1193 PubMed37102209

2023

Rapidly progressing generalized bullous fixed drug eruption after the first dose of COVID-19 messenger RNA vaccination.

Applications

Unspecified application

Species

Unspecified reactive species

Sooyeon Choi,Sung Hee Kim,Ji-Hye Hwang,Hyun Woo Jang,Sang Ho Oh,Do-Young Kim,Tae-Gyun Kim

Cancer immunology, immunotherapy : CII 70:1277-1289 PubMed33136178

2020

Granzyme B in peripheral blood mononuclear cells as a measure of cell-mediated immune response in paraneoplastic neurological syndromes and malignancy.

Applications

Unspecified application

Species

Unspecified reactive species

Mikołaj Piotr Zaborowski,Patrycja Stefens-Stawna,Krystyna Osztynowicz,Tomasz Piorunek,Halina Batura-Gabryel,Agnieszka Dyzmann-Sroka,Wojciech Kozubski,Ewa Nowak-Markwitz,Sławomir Michalak

Journal of immunology (Baltimore, Md. : 1950) 204:1373-1385 PubMed31969386

2020

Blockade of -Glycosylation Promotes Antitumor Immune Response of T Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shigemi Sasawatari,Yuki Okamoto,Atsushi Kumanogoh,Toshihiko Toyofuku

Experimental and therapeutic medicine 15:4979-4986 PubMed29805521

2018

Prognostic and therapeutic value of CD103 cells in renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Zhou,Lipeng Liu,Tuo Yang,Bingxin Lu

PLoS pathogens 13:e1006462 PubMed28640917

2017

Human hantavirus infection elicits pronounced redistribution of mononuclear phagocytes in peripheral blood and airways.

Applications

ELISA

Species

Human

Saskia Scholz,Faezzah Baharom,Gregory Rankin,Kimia T Maleki,Shawon Gupta,Sindhu Vangeti,Jamshid Pourazar,Andrea Discacciati,Jonas Höijer,Matteo Bottai,Niklas K Björkström,Johan Rasmuson,Magnus Evander,Anders Blomberg,Hans-Gustaf Ljunggren,Jonas Klingström,Clas Ahlm,Anna Smed-Sörensen

Scientific reports 7:88 PubMed28273905

2017

NKG2D modulates aggravation of liver inflammation by activating NK cells in HBV infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yadong Wang,Wei Wang,Chuan Shen,Yong Wang,Mingjing Jiao,Weiyan Yu,Hongzhu Yin,Xiaobo Shang,Qianfei Liang,Caiyan Zhao

Journal of immunology (Baltimore, Md. : 1950) 184:5885-94 PubMed20382885

2010

Mesenchymal stem cells protect breast cancer cells through regulatory T cells: role of mesenchymal stem cell-derived TGF-beta.

Applications

Unspecified application

Species

Human

Shyam A Patel,Justin R Meyer,Steven J Greco,Kelly E Corcoran,Margarette Bryan,Pranela Rameshwar

Immunology and cell biology 87:249-54 PubMed19079360

2008

Active and zymogen forms of granzyme B are constitutively released from cytotoxic lymphocytes in the absence of target cell engagement.

Applications

Unspecified application

Species

Human

Monica D Prakash,Catherina H Bird,Phillip I Bird

Journal of immunology (Baltimore, Md. : 1950) 181:3769-76 PubMed18768829

2008

Characterization of a novel nonclassical T cell clone with broad reactivity against human renal cell carcinomas.

Applications

Unspecified application

Species

Human

Qiong J Wang,Ken-Ichi Hanada,James C Yang
View all publications
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