JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314368

Human Granzyme K ELISA Kit

Be the first to review this product! Submit a review

|

(1 Publication)

Human Granzyme K ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Granzyme K with a sensitivity of 6.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

TRYP2, GZMK, Granzyme K, GrK, Fragmentin-3, Granzyme-3, NK-tryptase-2, NK-Tryp-2

3 Images
ELISA - Human Granzyme K ELISA Kit (AB314368)
  • ELISA

Supplier Data

ELISA - Human Granzyme K ELISA Kit (AB314368)
Sandwich ELISA - Human Granzyme K ELISA Kit (AB314368)
  • sELISA

Supplier Data

Sandwich ELISA - Human Granzyme K ELISA Kit (AB314368)

Interpolated concentrations of human Granzyme K in unstimulated and stimulated PBMC supernatant

Interpolated concentration of native Granzyme K was measured in duplicate at different sample concentrations. Undiluted samples are 100% unstimulated PBMC cell culture supernatant and stimulated PBMC cell culture supernatant (treated with 1.5% PHA-M for 46 hrs). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS

Sandwich ELISA - Human Granzyme K ELISA Kit (AB314368)
  • sELISA

Supplier Data

Sandwich ELISA - Human Granzyme K ELISA Kit (AB314368)

Example of human Granzyme K standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, Heparin Plasma, EDTA Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6.1 pg/mL

Range

34.375 - 2200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB100647

Human TGF beta 1 ELISA Kit

Sandwich ELISA - Human TGF beta 1 ELISA Kit (AB100647)

  • 5
  • 2
Assay Kits

AB282874

Mouse IFN gamma ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse IFN gamma ELISA Kit (AB282874)

  • 0
  • 0
Assay Kits

AB224877

Human IL-18BP ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IL-18BP ELISA Kit (AB224877)

  • 0
  • 0
Assay Kits

AB275898

Mouse IL-15 ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse IL-15 ELISA Kit (AB275898)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Granzyme K ELISA Kit ab314368 is a rapid single-wash 90-min Sandwich ELISA to measure Human Granzyme K in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 6.1 pg/mL.

How the assay works

Human Granzyme K SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Granzyme K ELISA Kit ab314368 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.1" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.71" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "83 - 93 %", "average": "= 89" }, { "sample": "Heparin Plasma", "range": "103 - 105 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "88 - 94 %", "average": "= 92" }, { "sample": "EDTA Plasma", "range": "98 - 106 %", "average": "= 101" }, { "sample": "Cell culture supernatant", "range": "93 - 100 %", "average": "= 97" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314368-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB314368-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB314368-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB314368-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB314368-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Granzyme K Lyophilized Recombinant Protein", "number":"AB314368-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Granzyme K Capture Antibody", "number":"AB314368-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB314368-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Granzyme K Detector Antibody", "number":"AB314368-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB314368-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB314368-CMP10", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Granzyme K also known as protein K or 24c3 is a serine protease with a mass of approximately 27 kilodaltons. It belongs to the granzyme family of proteins which are mainly found within the cytotoxic granules of natural killer (NK) cells and cytotoxic T lymphocytes (CTLs). These immune cells store granzyme K in their granules which they release to induce apoptosis in target cells. Researchers have identified granzyme K expression in various tissues and immune cells highlighting its widespread role in modulating immune responses.
Biological function summary

Granzyme K serves as a potent mediator in the immune system by inducing apoptosis in target cells. It does not act alone but rather in concert with other granzymes and proteins to effectively execute cell death. Granzyme K can degrade cellular proteins once introduced into a target cell's cytoplasm leading to apoptosis. The protease functions independently and does not require a complex for its activity contributing to the immune system's ability to eliminate infected or transformed cells.

Pathways

The granzyme K protein exists within essential immune regulatory pathways such as the cytotoxic T lymphocyte-induced apoptosis pathway and the natural killer cell-mediated cytotoxicity pathway. Granzyme K acts alongside granzymes A and B to promote programmed cell death and finely tune host immune responses. While granzymes A and B have more defined roles granzyme K complements their activity offering redundancy and ensuring efficient elimination of harmful target cells.

Granzyme K shows significant connections to inflammatory conditions and autoimmune diseases such as rheumatoid arthritis. Elevated granzyme K levels in these diseases may result from dysregulated immune responses leading to excessive tissue damage. In autoimmune disorders granzyme K works in combination with proteins like perforin which facilitates entry of granzymes into the cell to stimulate apoptosis. Understanding granzyme K's role in these conditions may help develop therapeutic approaches to modulate immune activity and minimize tissue damage.
style
left-column

Product protocols

style
left-column

Target data

Serine protease that initiates the GZMK pathway of the complement system, a cascade of proteins directly activated by CD8(+) T-cells that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 3262682, PubMed : 39814882, PubMed : 39914456). GZMK is specifically secreted by CD8(+) T-cells and mediates both recognition and initiation steps of GZMK complement pathway (PubMed : 39914456). First acts as a pattern recognition receptor, which specifically recognizes and binds heparan sulfate glycosaminoglycans on the pathogen surface to drive opsonization (PubMed : 39914456). It then initiates the complement pathway cascade by catalyzing cleavage and activation of C2 and C4, the next components of the complement pathway (PubMed : 39814882, PubMed : 39914456). GZMK-mediated complement activation is an important contributor to tissue inflammation (PubMed : 24711407, PubMed : 33271118, PubMed : 35704599, PubMed : 39614076, PubMed : 39814882, PubMed : 39914456). Also able to cleave and activate F2R/PAR1 and PAR2/F2RL1, possibly promoting interleukin release (PubMed : 21760880, PubMed : 26936634, PubMed : 35881628). May also regulate apoptosis by catalyzing cleavage and activation of BID, thereby promoting cytochrome C release from mitochrondria (PubMed : 17308307).
See full target information GZMK
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology, immunotherapy : CII 73:147 PubMed38833156

2024

SIRPG expression positively associates with an inflamed tumor microenvironment and response to PD-1 blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Libo Luo,Minlin Jiang,Hong Wu,Yiqiang Liu,Haowei Wang,Caicun Zhou,Shengxiang Ren,Xiaoxia Chen,Tao Jiang,Chuan Xu
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com