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AB313969

Human GRO ELISA Kit

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Human GRO ELISA Kit is a Sandwich ELISA for the measurement of Human GRO in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

GRO, GRO1, GROA, MGSA, SCYB1, CXCL1, Growth-regulated alpha protein, C-X-C motif chemokine 1, GRO-alpha(1-73), Melanoma growth stimulatory activity, Neutrophil-activating protein 3, NAP-3, GRO2, GROB, MIP2A, SCYB2, CXCL2, C-X-C motif chemokine 2, Growth-regulated protein beta, Macrophage inflammatory protein 2-alpha, Gro-beta, MIP2-alpha, GRO3, GROG, SCYB3, CXCL3, C-X-C motif chemokine 3, GRO-gamma(1-73), Growth-regulated protein gamma, Macrophage inflammatory protein 2-beta, GRO-gamma, MIP2-beta

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Sandwich ELISA - Human GRO ELISA Kit (AB313969)
  • sELISA

Supplier Data

Sandwich ELISA - Human GRO ELISA Kit (AB313969)

Example of standard curve using ab313969. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.6 ng/mL

Range

0.6 - 150 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human GRO ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GRO in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human GRO coated on a 96-well plate.

Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF6, FGF-7, G-CSF, GDNF, IFN-gamma, IGFBP-2, IGF-BP-3, IGF-BP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1alpha, MIP-1 beta, MIP-1gamma, PARC, PDGF, RANTES, SCF, SDF-1alpha, TARC, TGF-beta-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

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Recovery

[ { "sample": "Serum", "range": "87 - 146 %", "average": "= 123.4" }, { "sample": "Plasma", "range": "90 - 127 %", "average": "= 94.95" }, { "sample": "Cell culture media", "range": "84 - 104 %", "average": "= 106.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313969-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313969-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin", "number":"AB313969-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human GRO Protein Standard", "number":"AB313969-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313969-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human GRO Antibody-coated ELISA Plate", "number":"AB313969-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313969-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313969-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human GRO Detection Antibody", "number":"AB313969-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL2 also known as GRO gamma or GRO alpha is a small cytokine protein with a mass of approximately 7.9 kDa. It functions mechanically by binding to specific receptors on the surface of target cells playing important roles in immune response regulation and inflammation. The protein is expressed in various cell types including macrophages fibroblasts and epithelial cells. This factor is important for mediating chemotaxis stimulating leukocytes and acting as a potent chemoattractant for neutrophils.
Biological function summary

CXCL2 contributes to immune defense and tissue repair processes. The protein acts as a signaling molecule promoting migration and activation of immune cells to sites of inflammation. As part of the CXC chemokine family CXCL2 shares structural similarities with other chemokines but it does not form complexes. The molecule plays a role in mediating responses to environmental stimuli and participates in both innate and adaptive immunity.

Pathways

CXCL2 operates within pivotal chemokine signaling cascades and the inflammatory response. It is involved in the chemokine signaling pathway assisting in directing leukocytes to inflammatory sites. Related proteins like CXCL1 and CXCL8 often work alongside CXCL2 in coordinating immune responses. Furthermore CXCL2 activation can impact the NF-κB pathway a critical player in inflammation and immune response regulation integrating signals from various stress and inflammation contexts.

CXCL2 has associations with conditions such as rheumatoid arthritis and asthma. Elevated levels of this chemokine are often observed in inflamed joints of arthritis patients where it contributes to the recruitment of inflammatory cells exacerbating tissue damage. Additionally in asthma CXCL2 interacts with interleukin-6 contributing to airway inflammation and hyperreactivity. These interactions underline the chemokine’s role in chronic inflammatory disorders making it a possible target for therapeutic interventions.
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Product protocols

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Target data

Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
See full target information CXCL1

Additional targets

CXCL2,CXCL3
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Product promise

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