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AB190811

Human Growth Hormone ELISA Kit

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(13 Publications)

Human Growth Hormone ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Growth Hormone with a sensitivity of 1.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

View Alternative Names

Somatotropin, Growth hormone, Growth hormone 1, Pituitary growth hormone, GH, GH-N, GH1

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ELISA - Human Growth Hormone ELISA Kit (AB190811)
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ELISA - Human Growth Hormone ELISA Kit (AB190811)
Sandwich ELISA - Human Growth Hormone ELISA Kit (AB190811)
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Sandwich ELISA - Human Growth Hormone ELISA Kit (AB190811)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native Growth Hormone was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Growth Hormone ELISA Kit (AB190811)
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Sandwich ELISA - Human Growth Hormone ELISA Kit (AB190811)

Interpolated concentrations of native Growth Hormone in human plasma samples.

The concentrations of Growth Hormone were measured in duplicates, interpolated from the Growth Hormone standard curves and corrected for sample dilution. Undiluted samples are as follows : plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Growth Hormone concentration was determined to be 309.0 pg/mL in neat plasma (citrate), 329.1 pg/mL in neat plasma (heparin), and 120 pg/mL in and neat plasma (EDTA).

Sandwich ELISA - Human Growth Hormone ELISA Kit (AB190811)
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Sandwich ELISA - Human Growth Hormone ELISA Kit (AB190811)

Linearity of dilution.

Recombinant Growth Hormone was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

Sandwich ELISA - Human Growth Hormone ELISA Kit (AB190811)
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Sandwich ELISA - Human Growth Hormone ELISA Kit (AB190811)

Human Growth Hormone Standard Curve Comparison.

Standard Curve comparison between human Growth Hormone new SimpleStep ELISA kit and previous ELISA kit. The new SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human Growth Hormone ELISA Kit (AB190811)
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Sandwich ELISA - Human Growth Hormone ELISA Kit (AB190811)

Interpolated concentrations of native Growth Hormone in human normal mixed gender serum and pregnant serum samples.

The concentrations of Growth Hormone were measured in duplicates, interpolated from the Growth Hormone standard curves and corrected for sample dilution. Undiluted samples are as follows : mixed gender serum 100% and pregnant serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Growth Hormone concentration was determined to be 203.3 pg/mL in neat pooled mixed gender serum and 5,327 pg/mL in neat pooled pregnant female serum.

Sandwich ELISA - Human Growth Hormone ELISA Kit (AB190811)
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Supplier Data

Sandwich ELISA - Human Growth Hormone ELISA Kit (AB190811)

Serum from nine individual healthy human male and female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human Growth Hormone ELISA Kit (AB190811)
  • sELISA

Supplier Data

Sandwich ELISA - Human Growth Hormone ELISA Kit (AB190811)

Human Growth Hormone Standard Curve Comparison.

Standard Curve comparison between human Growth Hormone SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human Growth Hormone ELISA Kit (AB190811)
  • sELISA

Supplier Data

Sandwich ELISA - Human Growth Hormone ELISA Kit (AB190811)

Example of human Growth Hormone standard curve in Sample Diluent NS.

The Growth Hormone standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Growth Hormone ELISA Kit (AB190811)
  • sELISA

Supplier Data

Sandwich ELISA - Human Growth Hormone ELISA Kit (AB190811)

Example of human Growth Hormone standard curve in Sample Diluent NS. The Growth Hormone standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.6 pg/mL

Range

9.4 - 600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Growth Hormone ELISA Kit ab190811 is a rapid single-wash 90-min Sandwich ELISA to measure Human Growth Hormone in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, milk, serum, urine. This SimpleStep sensitivity is 1.6 pg/mL.

How the assay works

Human Growth Hormone SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Growth Hormone ELISA Kit ab190811 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free (Capture) ab244873
- Anti-Growth Hormone antibody [EPR9524-21] - BSA and Azide free (Detector) ab244873

Growth Hormone (also known as GH, GH1, Somatotropin and Pituitary growth hormone) is a circulations hormone that plays an important role in somatic growth control. Growth Hormone binds to the Growth Hormone Receptor present in a variety of tissues and induces signaling cascades. Growth Hormone secretion is controlled positively and negatively by other hormones, including Ghrelin and Somatostatin. Overproduction of Growth Hormone can result in gigantism whereas deficiency can contribute to dwarfism.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]
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Recovery

[ { "sample": "Milk", "range": "91 - 100 %", "average": "= 94" }, { "sample": "Serum", "range": "97 - 109 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "87 - 98 %", "average": "= 93" }, { "sample": "Urine", "range": "107 - 119 %", "average": "= 112" }, { "sample": "Heparin Plasma", "range": "94 - 99 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "92 - 102 %", "average": "= 97" }, { "sample": "Cell culture media", "range": "90 - 98 %", "average": "= 93" } ]
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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab190811-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB190811-CMP03" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB190811-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB190811-CMP04" }, { "size":"1 x 6000 µL", "name":"10X Human Growth Hormone Capture Antibody", "number":"AB190811-CMP09" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB190811-CMP05" }, { "size":"2 x 10 Vial", "name":"Human Growth Hormone Lyophilized Recombinant Protein (ab116162)", "number":"AB190811-CMP07" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB190811-CMP01" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB190811-CMP02" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB190811-CMP08" }, { "size":"1 x 6000 µL", "name":"10X Human Growth Hormone Detector Antibody", "number":"AB190811-CMP06" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab190811-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB190811-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB190811-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB190811-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB190811-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB190811-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Growth Hormone Capture Antibody", "number":"AB190811-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Growth Hormone Lyophilized Recombinant Protein (ab116162)", "number":"AB190811-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB190811-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Growth Hormone Detector Antibody", "number":"AB190811-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB190811-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Growth Hormone (GH) also known as somatotropin is a polypeptide hormone with a mass of approximately 22 kDa. The pituitary gland synthesizes and secretes GH. Once synthesized GH circulates in the bloodstream and affects various tissues throughout the body. GH's expression is tightly regulated because of its significant roles in growth processes. Researchers might use anti-growth hormone antibodies and various hGH testing kits including ELISA to study its levels and functions.
Biological function summary

GH triggers growth and cell reproduction by exerting effects on multiple tissues. It binds to the growth hormone receptor which initiates a cascade of reactions that ultimately stimulates growth in muscle bone and cartilage. GH also contributes to protein synthesis and helps regulate metabolism. GH acts mostly independently but can interact with cytokines and other hormones.

Pathways

GH plays important roles in the regulation of the JAK-STAT signaling pathway and the IGF-1 signaling pathway. The JAK-STAT pathway is important for transmitting the signal of GH binding to its receptor into cellular responses. GH stimulates IGF-1 production in the liver and IGF-1 then acts in an endocrine manner to mediate much of GH's growth-promoting activity. These interactions highlight the interconnectedness of GH with other hormones in growth signaling networks.

Several health conditions can arise from abnormal levels of GH. Dwarfism occurs due to GH deficiency while acromegaly results from GH excess. Clinical analysis might use hGH kits to measure hormone levels and aid in diagnosis. GH's role in such conditions links it to other hormones like insulin and glucagon that help regulate glucose levels further affecting metabolic processes. Recombinant forms of GH are used in therapy for these disorders providing avenues for treatment and management.
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Product protocols

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Target data

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
See full target information GH1
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Physiological research 73:739-753 PubMed39545789

2024

Supplementing With Which Form of Creatine (Hydrochloride or Monohydrate) Alongside Resistance Training Can Have More Impacts on Anabolic/Catabolic Hormones, Strength and Body Composition?

Applications

Unspecified application

Species

Unspecified reactive species

E Eghbali,H Arazi,K Suzuki

Aging cell 23:e14193 PubMed38724466

2024

Non-pituitary growth hormone enables colon cell senescence evasion.

Applications

Unspecified application

Species

Unspecified reactive species

Vera Chesnokova,Svetlana Zonis,Tugce Apaydin,Robert Barrett,Shlomo Melmed

Obesity (Silver Spring, Md.) 32:352-362 PubMed38018497

2023

Follistatin-like 1 is a myokine regulating lipid mobilization during endurance exercise and recovery.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Sun Nam,Su-Jeong Park,Chul Woo Ahn,Eun-Suk Cho,Hee-Joo Kim,YuSik Kim

EMBO reports 24:e56227 PubMed37341148

2023

Hypoxia causes pancreatic β-cell dysfunction and impairs insulin secretion by activating the transcriptional repressor BHLHE40.

Applications

Unspecified application

Species

Unspecified reactive species

Tomonori Tsuyama,Yoshifumi Sato,Tatsuya Yoshizawa,Takaaki Matsuoka,Kazuya Yamagata

Diabetes, metabolic syndrome and obesity : targets and therapy 15:3429-3436 PubMed36353668

2022

Relationship of Plasma Gremlin 1 Levels with Body Adiposity and Glycemic Control in Saudi Female Type 2 Diabetes Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Khalid A Al-Regaiey,Syed Shahid Habib,Ahmed R Alshamasi,Abdullah F Alnuwaybit,Bader A Alwhaibi,Naif M Alsulais,Abdullah I Alothman,Faisal M Alomar,Muhammad Iqbal

Andrologia 54:e14617 PubMed36257721

2022

Are androstenedione, dihydrotestosterone, thyroid-stimulating hormone, insulin-like growth factor I, and insulin-like growth factor binding protein 3 necessary for isolated micropenis healthy boys' evaluation without any phenotypic abnormalities? A cross-sectional study.

Applications

Unspecified application

Species

Unspecified reactive species

Soheila Siroosbakht,Sadra Rezakhaniha,Bijan Rezakhaniha

Frontiers in medicine 8:655863 PubMed34295909

2021

Low Growth Hormone Levels Predict Poor Outcome of Hepatitis B Virus-Related Acute-on-Chronic Liver Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Daxian Wu,Lingjian Zhang,Shanshan Ma,Yalei Zhao,Ronggao Chen,Fen Zhang,Qiuhong Liu,Xiaowei Xu,Zhongyang Xie

Nutrients 13: PubMed33804259

2021

Strategic Ingestion of High-Protein Dairy Milk during a Resistance Training Program Increases Lean Mass, Strength, and Power in Trained Young Males.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam Pourabbas,Reza Bagheri,Babak Hooshmand Moghadam,Darryn S Willoughby,Darren G Candow,Bradley T Elliott,Scott C Forbes,Damoon Ashtary-Larky,Mozhgan Eskandari,Alexei Wong,Frédéric Dutheil

Dose-response : a publication of International Hormesis Society 18:1559325820931262 PubMed32647498

2020

Individualized Whole-Body Vibration: Neuromuscular, Biochemical, Muscle Damage and Inflammatory Acute Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Riccardo Di Giminiani,Nadia Rucci,Lorenzo Capuano,Marco Ponzetti,Federica Aielli,Jozsef Tihanyi

BMC gastroenterology 20:199 PubMed32586279

2020

Effects of gastric sleeve surgery on the serum levels of GH, IGF-1 and IGF-binding protein 2 in healthy obese patients.

Applications

Unspecified application

Species

Unspecified reactive species

Khalid Al-Regaiey,Suad Alshubrami,Ibrahim Al-Beeshi,Torki Alnasser,Abdulnasser Alwabel,Hassan Al-Beladi,Omar Al-Tujjar,Abdulrahman Alnasser,Assim A Alfadda,Muhammad Iqbal
View all publications
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