Human Growth Hormone ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Growth Hormone with a sensitivity of 1.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations
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- EMBO reports 24:e562272023Hypoxia causes pancreatic β-cell dysfunction and impairs insulin secretion by activating the transcriptional repressor BHLHE40.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomonori Tsuyama,Yoshifumi Sato,Tatsuya Yoshizawa,Takaaki Matsuoka,Kazuya YamagataPubMed 37341148
- Diabetes, metabolic syndrome and obesity : targets and therapy 15:3429-34362022Relationship of Plasma Gremlin 1 Levels with Body Adiposity and Glycemic Control in Saudi Female Type 2 Diabetes Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKhalid A Al-Regaiey,Syed Shahid Habib,Ahmed R Alshamasi,Abdullah F Alnuwaybit,Bader A Alwhaibi,Naif M Alsulais,Abdullah I Alothman,Faisal M Alomar,Muhammad IqbalPubMed 36353668
- Andrologia 54:e146172022Are androstenedione, dihydrotestosterone, thyroid-stimulating hormone, insulin-like growth factor I, and insulin-like growth factor binding protein 3 necessary for isolated micropenis healthy boys' evaluation without any phenotypic abnormalities? A cross-sectional study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSoheila Siroosbakht,Sadra Rezakhaniha,Bijan RezakhanihaPubMed 36257721
- Frontiers in medicine 8:6558632021Low Growth Hormone Levels Predict Poor Outcome of Hepatitis B Virus-Related Acute-on-Chronic Liver Failure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaxian Wu,Lingjian Zhang,Shanshan Ma,Yalei Zhao,Ronggao Chen,Fen Zhang,Qiuhong Liu,Xiaowei Xu,Zhongyang XiePubMed 34295909
- Nutrients 13:2021Strategic Ingestion of High-Protein Dairy Milk during a Resistance Training Program Increases Lean Mass, Strength, and Power in Trained Young Males.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaryam Pourabbas,Reza Bagheri,Babak Hooshmand Moghadam,Darryn S Willoughby,Darren G Candow,Bradley T Elliott,Scott C Forbes,Damoon Ashtary-Larky,Mozhgan Eskandari,Alexei Wong,Frédéric DutheilPubMed 33804259
- BMC gastroenterology 20:1992020Effects of gastric sleeve surgery on the serum levels of GH, IGF-1 and IGF-binding protein 2 in healthy obese patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKhalid Al-Regaiey et. al.PubMed 32586279
- Scientific reports 10:54952020Obestatin and growth hormone reveal the interaction of central obesity and other cardiometabolic risk factors of metabolic syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAngus P Yu,Felix N Ugwu,Bjorn T Tam,Paul H Lee,Vicki Ma,Simon Pang,Angel S Chow,Kenneth K Cheng,Christopher W Lai,Cesar S Wong,Parco M SiuPubMed 32218464
- International journal of molecular medicine 45:931-9382020Non‑toxic sulfur enhances growth hormone signaling through the JAK2/STAT5b/IGF‑1 pathway in C2C12 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Young Kang,Nipin Sp,Eun Seong Jo,Hyoung Do Kim,Il Ho Kim,Se Won Bae,Kyoung-Jin Jang,Young Mok YangPubMed 31894268
- Scientific reports 7:23972017Extensive phenotypic characterization of a new transgenic mouse reveals pleiotropic perturbations in physiology due to mesenchymal hGH minigene expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAimilios Kaklamanos et. al.PubMed 28546545
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 9.4 - 600 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1.6 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
Alternative names
Somatotropin, Growth hormone, Growth hormone 1, Pituitary growth hormone, GH, GH-N, GH1
What's included?
Recommended products
Human Growth Hormone ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Growth Hormone with a sensitivity of 1.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 9.4 - 600 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1.6 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 3.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 2.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Milk | Average % = 94 | Range 91 - 100 % |
Sample type Serum | Average % = 103 | Range 97 - 109 % |
Sample type EDTA Plasma | Average % = 93 | Range 87 - 98 % |
Sample type Urine | Average % = 112 | Range 107 - 119 % |
Sample type Heparin Plasma | Average % = 97 | Range 94 - 99 % |
Sample type Citrate plasma | Average % = 97 | Range 92 - 102 % |
Sample type Cell culture media | Average % = 93 | Range 90 - 98 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Growth Hormone ELISA Kit (ab190811) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Growth Hormone protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, milk, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Growth Hormone with 1.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY
This kit recognizes both native and recombinant human Growth Hormone protein in serum, plasma, milk, urine, and cell culture supernatant samples only.
Saliva, and cell and tissue extract samples have not been tested with this kit.
SPECIES REACTIVITY
This kit recognizes human Growth Hormone protein.
Other species reactivity was determined by measuring neat serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.
Reactivity < 3% was determined for the following species:
- Mouse
- Rat
- Cow
CALIBRATION
This immunoassay is calibrated against a highly purified human Growth Hormone. The NIBSC/WHO unclassified purified human Growth Hormone preparation 80/505 was evaluated in this kit.
The dose response curve of the unclassified standard Growth Hormone parallels the SimpleStep standard curve. To convert sample values obtained with the SimpleStep Human Growth Hormone kit to approximate NIBSC 80/505 units, use the equation below.
NIBSC (80/505) approximate value (mIU/mL) = 2.3x10-6 x SimpleStep Human Growth Hormone value (pg/mL).
Growth Hormone (also known as GH, GH1, Somatotropin and Pituitary growth hormone) is a circulations hormone that plays an important role in somatic growth control. Growth Hormone binds to the Growth Hormone Receptor present in a variety of tissues and induces signaling cascades. Growth Hormone secretion is controlled positively and negatively by other hormones, including Ghrelin and Somatostatin. Overproduction of Growth Hormone can result in gigantism whereas deficiency can contribute to dwarfism.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Growth Hormone (GH) also known as somatotropin is a polypeptide hormone with a mass of approximately 22 kDa. The pituitary gland synthesizes and secretes GH. Once synthesized GH circulates in the bloodstream and affects various tissues throughout the body. GH's expression is tightly regulated because of its significant roles in growth processes. Researchers might use anti-growth hormone antibodies and various hGH testing kits including ELISA to study its levels and functions.
- Biological function summary
GH triggers growth and cell reproduction by exerting effects on multiple tissues. It binds to the growth hormone receptor which initiates a cascade of reactions that ultimately stimulates growth in muscle bone and cartilage. GH also contributes to protein synthesis and helps regulate metabolism. GH acts mostly independently but can interact with cytokines and other hormones.
- Pathways
GH plays important roles in the regulation of the JAK-STAT signaling pathway and the IGF-1 signaling pathway. The JAK-STAT pathway is important for transmitting the signal of GH binding to its receptor into cellular responses. GH stimulates IGF-1 production in the liver and IGF-1 then acts in an endocrine manner to mediate much of GH’s growth-promoting activity. These interactions highlight the interconnectedness of GH with other hormones in growth signaling networks.
- Associated diseases and disorders
Several health conditions can arise from abnormal levels of GH. Dwarfism occurs due to GH deficiency while acromegaly results from GH excess. Clinical analysis might use hGH kits to measure hormone levels and aid in diagnosis. GH's role in such conditions links it to other hormones like insulin and glucagon that help regulate glucose levels further affecting metabolic processes. Recombinant forms of GH are used in therapy for these disorders providing avenues for treatment and management.
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9 product images
Sandwich ELISA - Human Growth Hormone ELISA Kit (ab190811)
Human Growth Hormone Standard Curve Comparison.
Standard Curve comparison between human Growth Hormone SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human Growth Hormone ELISA Kit (ab190811)
Human Growth Hormone Standard Curve Comparison.
Standard Curve comparison between human Growth Hormone new SimpleStep ELISA kit and previous ELISA kit. The new SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human Growth Hormone ELISA Kit (ab190811)
Example of human Growth Hormone standard curve in Sample Diluent NS.
The Growth Hormone standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Growth Hormone ELISA Kit (ab190811)
Interpolated concentrations of native Growth Hormone in human normal mixed gender serum and pregnant serum samples.
The concentrations of Growth Hormone were measured in duplicates, interpolated from the Growth Hormone standard curves and corrected for sample dilution. Undiluted samples are as follows: mixed gender serum 100% and pregnant serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Growth Hormone concentration was determined to be 203.3 pg/mL in neat pooled mixed gender serum and 5,327 pg/mL in neat pooled pregnant female serum.
Sandwich ELISA - Human Growth Hormone ELISA Kit (ab190811)
Interpolated concentrations of native Growth Hormone in human plasma samples.
The concentrations of Growth Hormone were measured in duplicates, interpolated from the Growth Hormone standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Growth Hormone concentration was determined to be 309.0 pg/mL in neat plasma (citrate), 329.1 pg/mL in neat plasma (heparin), and 120 pg/mL in and neat plasma (EDTA).
Sandwich ELISA - Human Growth Hormone ELISA Kit (ab190811)
Serum from nine individual healthy human male and female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human Growth Hormone ELISA Kit (ab190811)
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Growth Hormone was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Growth Hormone ELISA Kit (ab190811)
Linearity of dilution.
Recombinant Growth Hormone was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.
Sandwich ELISA - Human Growth Hormone ELISA Kit (ab190811)
Example of human Growth Hormone standard curve in Sample Diluent NS. The Growth Hormone standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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