Human GRP78 BiP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human GRP78 BiP with a sensitivity of 0.051 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
GRP78, HSPA5, Endoplasmic reticulum chaperone BiP, 78 kDa glucose-regulated protein, Binding-immunoglobulin protein, Heat shock protein 70 family protein 5, Heat shock protein family A member 5, Immunoglobulin heavy chain-binding protein, GRP-78, BiP, HSP70 family protein 5
- sELISA
Supplier Data
Sandwich ELISA - Human GRP78 BiP ELISA Kit (AB302765)
The concentration of GRP78 BiP was measured in human Hela treated (2 µg/mL tunicamycin for 8 hours) cell extract and Hela untreated cell extracts based on a 5 µg/mL extract load. The mean concentration was determined to be 2.19 ng/mL in untreated Hela cell extract and 7.72 ng/mL in treated Hela cell extract.
- sELISA
Supplier Data
Sandwich ELISA - Human GRP78 BiP ELISA Kit (AB302765)
Example of human GRP78 BiP standard curve. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human GRP78 BiP ELISA Kit (AB302765)
Interpolated concentration of native GRP78 BiP was measured in duplicate at different sample concentrations. Undiluted samples are as follows : tunicamycin treated Hela cell extract 5 µg/mL, and HepG2 cell extract 1.5 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
- sELISA
Supplier Data
Sandwich ELISA - Human GRP78 BiP ELISA Kit (AB302765)
Interpolated concentration of native GRP78 BiP was measured in duplicate at different sample concentrations. Undiluted samples are as follows : liver extract 5 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Complementary Products
Primary Antibodies
AB81283
Anti-AKT1 (phospho S473) antibody [EP2109Y]
RabMAb|Recombinant|Lab Essentials|20ul selling size
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho S473) antibody [EP2109Y] (AB81283)](https://content.abcam.com/products/images/akt1-phospho-s473-antibody-ep2109y-ab81283--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98892.gif)
- 4
- 10
Reactivity data
Product details
Human GRP78 BiP SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human GRP78 BiP protein in human cell extract and tissue extract. Quantitate Human GRP78 BiP with 0.051 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GRP78/BiP is important in the unfolded protein response (UPR) a cellular stress response related to the ER. It is part of a complex that manages protein load inside the ER by regulating the fold of nascent proteins and interacting with other ER stress sensors like IRE1 PERK and ATF6. This function is essential for maintaining proper protein conformation in the ER especially during physiological stress ensuring that only correctly folded proteins proceed to the Golgi apparatus.
Pathways
GRP78/BiP significantly affects the UPR and apoptosis pathways. By controlling protein folding and quality in the ER GRP78 helps prevent cell death under stress conditions. It works closely with other proteins like ATF6 which activates stress response genes. In normal conditions GRP78 keeps stress transducers inactivated but during stress it dissociates allowing UPR signaling to occur.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com