JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB284002

Human GRX2 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human GRX2 ELISA Kit is a Sandwich ELISA for the measurement of Human GRX2 in Human in Cell/Tissue Extracts samples.

View Alternative Names

GRX2, CGI-133, GLRX2

1 Images
Sandwich ELISA - Human GRX2 ELISA Kit (AB284002)
  • sELISA

Supplier Data

Sandwich ELISA - Human GRX2 ELISA Kit (AB284002)

Example of Human GRX2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Qualitative

Sensitivity

= 0.3 ng/mL

Range

0.038 - 2.4 ng/mL

Assay time

4h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB283988

Human Peroxiredoxin 5 ELISA Kit

Sandwich ELISA - Human Peroxiredoxin 5 ELISA Kit (AB283988)

  • 0
  • 0
Assay Kits

AB277423

Human GST3 / GST pi ELISA Kit

ELISA - Human GST3 / GST pi ELISA Kit (AB277423)

  • 0
  • 0
Assay Kits

AB316899

Human Superoxide Dismutase 3/EC-SOD ELISA Kit

SimpleStep

Sandwich ELISA - Human Superoxide Dismutase 3/EC-SOD ELISA Kit (AB316899)

  • 0
  • 0
Assay Kits

AB227895

Human SIRT2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human SIRT2 ELISA Kit (AB227895)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human GRX2 ELISA Kit (ab284002) is designed for detection of glutaredoxin-2 in human cell lysate samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human glutaredoxin-2 in approximately 4 hours.

A polyclonal antibody specific for human glutaredoxin-2 has been pre-coated onto a 96-well microplate with removable strips. Glutaredoxin-2 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human glutaredoxin-2, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab284002-1x96Tests", "assetComponentDetails": [ { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB284002-CMP10", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB284002-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB284002-CMP01", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB284002-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB284002-CMP04", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB284002-CMP06", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB284002-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"GRX2 Standard", "number":"AB284002-CMP05", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human GRX2 Antibody", "number":"AB284002-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human GRX2 Microplate", "number":"AB284002-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target GRX2 also commonly known as GLRX2 is a human protein encoded by the GLRX2 gene. GRX2 functions as a glutaredoxin a type of enzyme involved in the process of reducing disulfide bonds in proteins which is essential for cell redox regulation. The protein has a molecular mass of approximately 16 kDa and primarily resides in the mitochondria and cytoplasm of cells. Its expression occurs in various tissues with significant levels in the heart brain and skeletal muscle indicating its broad functional roles across different organ systems.
Biological function summary

The GRX2 protein plays a critical role in maintaining cellular redox balance by facilitating the reversible oxidation of protein cysteines. This is important for protecting cells against oxidative stress. GRX2 does not function as an isolated entity but instead forms part of larger multi-protein complexes interacting with other enzymes to regulate cellular homeostasis. These interactions help to protect cells from oxidative damage promote protein folding and regenerate other antioxidants.

Pathways

GRX2 holds a significant position in the redox regulation and antioxidant pathways. It partners with thioredoxin reductase and glutathione systems to mediate these pathways and manage cellular oxidative stress. The protein's activity is closely tied to glutathione with GRX2 often acting in synergy with glutathione to reduce complex oxidized protein thiols. This collaboration is vital in the oxidative stress response where the pathway elements must efficiently counterbalance oxidative challenges.

GRX2's function links it to several oxidative stress-related conditions including neurodegenerative diseases and cardiovascular disorders. In neurodegenerative diseases like Parkinson's disease GRX2's ability to combat oxidative stress is compromised leading to neuronal damage. Additionally the protein's interaction with other redox regulatory proteins such as thioredoxin suggests interconnected roles in the progression of these disorders. Cardiovascular diseases also involve GRX2 due to its essential function in maintaining cardiovascular integrity under environmental and metabolic oxidative stress.
style
left-column

Product protocols

style
left-column

Target data

Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.
See full target information GLRX2
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com