Human GSTA1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human GSTA1 with a sensitivity of 14 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Cell culture extracts, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.39 - 25 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 14 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis (PubMed:11152686). Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid (PubMed:16624487).
Alternative names
Glutathione S-transferase A1, 13-hydroperoxyoctadecadienoate peroxidase, GST HA subunit 1, GST class-alpha member 1, GST-epsilon, GSTA1-1, GTH1, GSTA1
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Recommended products
Human GSTA1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human GSTA1 with a sensitivity of 14 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Cell culture extracts, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.39 - 25 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 14 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Urine | n 8 | mean - | SD - | C.V. 5.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Urine | n 3 | mean - | SD - | C.V. 9.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture extracts | Average % = 104 | Range 89 - 111 % |
Sample type Urine | Average % = 97 | Range 89 - 104 % |
Sample type Tissue Extracts | Average % = 94 | Range 88 - 100 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human GSTA1 ELISA Kit (ab245717) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GSTA1 protein in tissue extracts, urine, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human GSTA1 with 14 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The GSTA1 protein also known as Glutathione S-transferase alpha 1 is an important enzyme in detoxification processes in the body. It has a molecular mass of approximately 25 kDa and expresses highly in the liver but is also seen in other tissues like the kidney and lung. It belongs to the glutathione S-transferase family which plays function in the conjugation of reduced glutathione to a variety of endogenous and exogenous electrophilic compounds.
- Biological function summary
The GSTA1 protein contributes significantly to the neutralization of oxidative stress by detoxifying harmful compounds. This enzyme shows a high affinity for certain substrates and serves as a detoxifying agent particularly in the liver safeguarding cells from oxidative damage. GSTA1 exists mostly as a homodimer but can also form heterodimers with other related enzymes enhancing its substrate range and activity.
- Pathways
GSTA1 plays a role in metabolic and detoxification pathways essential for cellular protection. It is an important player in the glutathione metabolism pathway where it collaborates with other enzymes like GSTM1 to facilitate detoxification processes. Additionally GSTA1 participates in the pathways leading to the synthesis of mercapturic acids which are important for the excretion of toxic metabolites.
- Associated diseases and disorders
GSTA1 is linked to conditions such as cancer and cardiovascular diseases. Altered expression or polymorphisms in the GSTA1 gene can affect the enzyme's ability to detoxify carcinogens making it significant in cancer risk and progression. The GSTA1 protein also interacts with other enzymes like GSTA4 in the context of oxidative stress which relates to cardiovascular disorder pathogenesis.
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3 product images
Sandwich ELISA - Human GSTA1 ELISA Kit (ab245717)
Example of human GSTA1 standard curve in Sample Diluent NS. The GSTA1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human GSTA1 ELISA Kit (ab245717)
Example of human GSTA1 standard curve in 1X Cell Extraction Buffer PTR. The GSTA1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human GSTA1 ELISA Kit (ab245717)
Interpolated concentrations of native GSTA1 in human urine, liver and HepG2 cell extract samples.
The concentrations of GSTA1 were measured in duplicates, interpolated from the GSTA1 standard curves and corrected for sample dilution. Undiluted samples are as follows: 25% urine, 0.125 μg/mL liver, and 100 μg/mL HepG2 cell supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GSTA1 concentration was determined to be 125 ng/mL in urine.
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