Human GSTM1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human GSTM1 in Human in Plasma, Cell culture supernatant, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2.048 - 500 ng/mL
- Sensitivity
- = 2 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed:21046276).
Alternative names
GST1, GSTM1, Glutathione S-transferase Mu 1, GST HB subunit 4, GST class-mu 1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4
What's included?
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Human GSTM1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human GSTM1 in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2.048 - 500 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 2 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 94.36 | Range 69 - 137 % |
Sample type Plasma | Average % = 82.25 | Range 69 - 126 % |
Sample type Cell culture media | Average % = 88.87 | Range 69 - 126 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human GSTM1 ELISA Kit (ab277475) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human GSTM1 in serum (human GSTM1 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Human GSTM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human GSTM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human GSTM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human GSTM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The GSTM1 protein also known as Glutathione S-transferase Mu 1 plays important roles in cellular detoxification processes. It belongs to the glutathione S-transferase family of enzymes which are involved in the conjugation of reduced glutathione to xenobiotic substrates for the purpose of detoxification. GSTM1 has a molecular mass of approximately 26 kDa. This protein expresses mostly in liver and kidney tissues but has presence in other tissues like lung and testis as well.
- Biological function summary
GSTM1 acts in metabolism of endogenous and xenobiotic compounds. It does not work alone but rather functions as part of enzyme complexes that alter chemical structure of toxic substances making them safer for cellular systems. GSTM1 helps in protecting cells from damage caused by oxidative stress. The interaction of this protein with glutathione provides a protective mechanism against toxic chemicals such as carcinogens and electrophiles.
- Pathways
GSTM1 has significant involvement in metabolic detoxification and the glutathione-mediated pathway. It plays a role in the metabolism of drugs and clearance of harmful compounds in these pathways. It often interacts with other proteins like GSTP1 and GSTT1 within these pathways working together to facilitate cellular defense mechanisms. GSTM1 contributes importantly to maintaining cellular redox homeostasis by its enzymatic activities.
- Associated diseases and disorders
GSTM1 shows a connection to various cancers and cardiovascular diseases. The absence or null genotype of GSTM1 associates with higher risk of developing certain cancers like bladder and lung cancer due to impaired detoxification capacity. In cardiovascular diseases the protein's deficiency can disrupt normal oxidative stress balance. GSTP1 operates alongside GSTM1 in pathological situations with their combined functions influencing disease susceptibility and progression.
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ELISA - Human GSTM1 ELISA Kit (ab277475)
These standard curves are for demonstration only. A standard curve must be run with each assay.
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Product protocols
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