JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB277475

Human GSTM1 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human GSTM1 ELISA Kit is a Sandwich ELISA for the measurement of Human GSTM1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

GST1, GSTM1, Glutathione S-transferase Mu 1, GST HB subunit 4, GST class-mu 1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4

1 Images
ELISA - Human GSTM1 ELISA Kit (AB277475)
  • ELISA

Supplier Data

ELISA - Human GSTM1 ELISA Kit (AB277475)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2 ng/mL

Range

2.048 - 500 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB178684

Anti-GSTM1/2/4/5 antibody [EPR13338]

RabMAb|Recombinant

Immunoprecipitation - Anti-GSTM1/2/4/5 antibody [EPR13338] (AB178684)

  • 0
  • 0
Proteins & Peptides

AB113188

Recombinant Human Heme Oxygenase 1 protein

Western blot - Recombinant Human Heme Oxygenase 1 protein (AB113188)

  • 0
  • 0
Primary Antibodies

AB78316

Anti-p53 (acetyl K120) antibody [10E5]

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-p53 (acetyl K120) antibody [10E5] (AB78316)

  • 4
  • 4
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human GSTM1 ELISA Kit (ab277475) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human GSTM1 in serum (human GSTM1 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for Human GSTM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human GSTM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human GSTM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human GSTM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "69 - 137 %", "average": "= 94.36" }, { "sample": "Plasma", "range": "69 - 126 %", "average": "= 82.25" }, { "sample": "Cell culture media", "range": "69 - 126 %", "average": "= 88.87" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277475-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277475-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277475-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB277475-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277475-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human GSTM1 coated Microplate", "number":"AB277475-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human GSTM1 standard protein (Lyophilized)", "number":"AB277475-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277475-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human GSTM1 Antibody", "number":"AB277475-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GSTM1 protein also known as Glutathione S-transferase Mu 1 plays important roles in cellular detoxification processes. It belongs to the glutathione S-transferase family of enzymes which are involved in the conjugation of reduced glutathione to xenobiotic substrates for the purpose of detoxification. GSTM1 has a molecular mass of approximately 26 kDa. This protein expresses mostly in liver and kidney tissues but has presence in other tissues like lung and testis as well.
Biological function summary

GSTM1 acts in metabolism of endogenous and xenobiotic compounds. It does not work alone but rather functions as part of enzyme complexes that alter chemical structure of toxic substances making them safer for cellular systems. GSTM1 helps in protecting cells from damage caused by oxidative stress. The interaction of this protein with glutathione provides a protective mechanism against toxic chemicals such as carcinogens and electrophiles.

Pathways

GSTM1 has significant involvement in metabolic detoxification and the glutathione-mediated pathway. It plays a role in the metabolism of drugs and clearance of harmful compounds in these pathways. It often interacts with other proteins like GSTP1 and GSTT1 within these pathways working together to facilitate cellular defense mechanisms. GSTM1 contributes importantly to maintaining cellular redox homeostasis by its enzymatic activities.

GSTM1 shows a connection to various cancers and cardiovascular diseases. The absence or null genotype of GSTM1 associates with higher risk of developing certain cancers like bladder and lung cancer due to impaired detoxification capacity. In cardiovascular diseases the protein's deficiency can disrupt normal oxidative stress balance. GSTP1 operates alongside GSTM1 in pathological situations with their combined functions influencing disease susceptibility and progression.
style
left-column

Product protocols

style
left-column

Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed : 9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed : 21046276).
See full target information GSTM1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com