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AB284003

Human GSTZ1 ELISA Kit

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Human GSTZ1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human GSTZ1 in Human in Cell/Tissue Extracts samples.

View Alternative Names

MAAI, GSTZ1, Maleylacetoacetate isomerase, GSTZ1-1, Glutathione S-transferase zeta 1

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Sandwich ELISA - Human GSTZ1 ELISA Kit (AB284003)
  • sELISA

Supplier Data

Sandwich ELISA - Human GSTZ1 ELISA Kit (AB284003)

Example of Human GSTZ1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 54 pg/mL

Range

0.125 - 8 ng/mL

Assay time

4h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Glutathione S-Transferase Zeta 1 (GSTZ1) ELISA Kit (ab284003) is designed for detection of GSTZ1 in human cell lysate samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human GSTZ1 in approximately 4 hours.

A polyclonal antibody specific for human GSTZ1 has been pre-coated onto a 96-well microplate with removable strips. GSTZ1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human GSTZ1, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab284003-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB284003-CMP06", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB284003-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB284003-CMP04", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human GSTZ1 Antibody", "number":"AB284003-CMP07", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB284003-CMP10", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB284003-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB284003-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"GSTZ1 Standard", "number":"AB284003-CMP05", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB284003-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human GSTZ1 Microplate", "number":"AB284003-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GSTZ1 also known as Glutathione S-Transferase Zeta 1 is an enzyme that plays an important role in the metabolism of various chemical compounds. It has a molecular mass of approximately 34 kDa. GSTZ1 is ubiquitously expressed in many tissues with significant levels in the liver kidney and heart. This enzyme is part of the GST family which contributes to detoxification processes by conjugating reduced glutathione to various substrates.
Biological function summary

GSTZ1 participates in the breakdown of amino acids specifically in the catabolic pathway of L-phenylalanine and L-tyrosine. The enzyme assists in the production of maleylacetoacetate and its isomerization to fumarylacetoacetate an essential step in the phenylalanine and tyrosine catabolism. GSTZ1 does not form part of a larger multi-protein complex but its function is critical in maintaining cellular homeostasis by preventing the accumulation of potentially toxic intermediates.

Pathways

GSTZ1 integrates into the metabolic pathway that includes glutathione metabolism and the phenylalanine and tyrosine degradation pathway. It interacts functionally with other enzymes like Fumarylacetoacetate hydrolase (FAH) and Phenylalanine hydroxylase (PAH) playing roles in sequential catalysis steps. These pathways are vital for maintaining amino acid balance and detoxification of byproducts within the organism.

GSTZ1 has been linked to metabolic disorders like Tyrosinemia type I and various forms of cancer. Tyrosinemia type I a disorder caused by defects in tyrosine metabolism directly implicates GSTZ1 as an important player in managing toxic intermediates. Additionally altered expression of GSTZ1 has been observed in different cancer types suggesting its potential role in tumorigenesis. FAH related through the Tyrosinemia disorder represents another key protein that GSTZ1 activity impacts highlighting its disease association.
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Product protocols

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Target data

Bifunctional enzyme showing minimal glutathione-conjugating activity with ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with T-butyl and cumene hydroperoxides. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid.
See full target information GSTZ1
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