AB243682

Human H-FABP ELISA Kit

Recombinant
SimpleStep
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(5 Publications)

Human H-FABP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human H-FABP with a sensitivity of 2.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

FABP11, MDGI, FABP3, Fatty acid-binding protein 3, Heart-type fatty acid-binding protein, Mammary-derived growth inhibitor, Muscle fatty acid-binding protein, H-FABP, M-FABP

6 Images

Sandwich ELISA - Human H-FABP ELISA Kit (AB243682)

sELISA

Supplier Data

Sandwich ELISA - Human H-FABP ELISA Kit (AB243682)

Example of human H-FABP standard curve in Sample Diluent NS.

The H-FABP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human H-FABP ELISA Kit (AB243682)

Example of human H-FABP standard curve in 1X cell extraction buffer PTR.

The H-FABP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human H-FABP ELISA Kit (AB243682)

Interpolated concentrations of native H-FABP in human milk sample.

The concentrations of H-FABP were measured in duplicates, interpolated from the H-FABP standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 1 : 50,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 4,273 ng/mL in milk 1 : 100,000.

sELISA

Supplier Data

Sandwich ELISA - Human H-FABP ELISA Kit (AB243682)

Interpolated concentrations of native H-FABP in human heart extract based on a 13 ng/mL extract load.

The concentrations of H-FABP were measured in duplicate and interpolated from the H-FABP standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human H-FABP ELISA Kit (AB243682)

Interpolated concentrations of native H-FABP in human serum, plasma and CSF samples.

The concentrations of H-FABP were measured in duplicates, interpolated from the H-FABP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (heparin) 25%, cerebrospinal fluid 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 1.93 ng/mL in serum, 2.05 ng/mL in plasma (citrate), 1.57 ng/mL in plasma (heparin), and 1.3 ng/mL in cerebrospinal fluid.

sELISA

Supplier Data

Sandwich ELISA - Human H-FABP ELISA Kit (AB243682)

Serum from eight individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 1,635.8 pg/mL with a range of 873 – 2,940 pg/mL.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Tissue Extracts, Heparin Plasma, Citrate plasma, Milk, Serum

Reacts with

Cow, Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.4 pg/mL

Range

10.9 - 700 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human H-FABP ELISA Kit (ab243682) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of H-FABP protein in hep plasma, milk, serum, tissue extracts, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human H-FABP with 2.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 2, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]

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Recovery

[ { "sample": "Serum", "range": "82 - 90 %", "average": "= 86" }, { "sample": "Milk", "range": "82 - 94 %", "average": "= 87" }, { "sample": "Cerebral Spinal Fluid", "range": "107 - 113 %", "average": "= 110" }, { "sample": "Tissue Extracts", "range": "104 - 108 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "89 - 94 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "95 - 103 %", "average": "= 100" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

H-FABP also known as heart-type fatty acid binding protein is a small protein with a molecular mass of approximately 14.5 kDa. This protein is primarily found in the cytoplasm of cardiac muscle cells but it also can be detected in skeletal muscles and other tissues at lower concentrations. H-FABP binds long-chain fatty acids and is important for the intracellular transport and metabolism of these molecules. The protein is often used as a biomarker for cardiac injury due to its rapid release into the bloodstream following muscle damage.

Biological function summary

Fatty acid binding largely defines H-FABP's role facilitating the transport of fatty acids within cells which is key for energy production. It is not part of a larger protein complex but plays into broader lipid metabolism processes. The efficiency of H-FABP in fatty acid binding and transport helps maintain cellular energy homeostasis especially in tissues with high fatty acid metabolism like the heart.

Pathways

H-FABP integrates into the fatty acid metabolic pathways and the beta-oxidation pathway. It is associated with the transport and utilization of fatty acids linking it to the peroxisome proliferator-activated receptor (PPAR) signaling pathway an important regulator of lipid metabolism. Proteins such as CD36 and PPARα interact with H-FABP within these pathways influencing lipid storage and energy balance.

Disruptions in H-FABP levels or function correlate with cardiovascular diseases including myocardial infarction. Elevated blood levels of H-FABP provide early markers of cardiac cell injury. Additionally alterations can also relate to metabolic disorders like obesity where lipid metabolism gets impaired. In these contexts proteins such as troponin and creatine kinase may also serve as biomarkers elucidating the relationship between H-FABP and myocardial stress or damage.

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Product protocols

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Target data

FABPs are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.

See full target information FABP3

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Heart and vessels 39:206-215 PubMed37957288

2023

Biomarker array for prediction of acute kidney injury after percutaneous coronary intervention for patients who had acute ST segment elevation myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Amr Alkassas,Yasser Elbarbary,Mohammed H Sherif,Shaimaa B El-Saied,Rasha Y Hagag,Mohammed Elbarbary

Experimental biology and medicine (Maywood, N.J.) 248:532-540 PubMed36803120

2023

Plasma Galectin-3 and H-FABP correlate with poor physical performance in patients with congestive heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Firdos Ahmad,Asima Karim,Javaidullah Khan,Rizwan Qaisar

Cell death & disease 13:671 PubMed35918330

2022

BET bromodomain inhibition rescues PD-1-mediated T-cell exhaustion in acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjun Zhong,Rili Gao,Ruocong Zhao,Youxue Huang,Cunte Chen,Kehan Li,Xibao Yu,Dingrui Nie,Zheng Chen,Xin Liu,Zhuandi Liu,Shaohua Chen,Yuhong Lu,Zhi Yu,Liang Wang,Peng Li,Chengwu Zeng,Yangqiu Li

Biosensors 12: PubMed35323442

2022

Towards a Point-of-Care (POC) Diagnostic Platform for the Multiplex Electrochemiluminescent (ECL) Sensing of Mild Traumatic Brain Injury (mTBI) Biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Milica Jović,Denis Prim,Edis Saini,Marc Emil Pfeifer

Scientific reports 11:8632 PubMed33883602

2021

Prediction of sarcopenia using a battery of circulating biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Rizwan Qaisar,Asima Karim,Tahir Muhammad,Islam Shah,Javaidullah Khan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human H-FABP ELISA Kit

Sandwich ELISA - Human H-FABP ELISA Kit (AB243682)

Sandwich ELISA - Human H-FABP ELISA Kit (AB243682)

Sandwich ELISA - Human H-FABP ELISA Kit (AB243682)

Sandwich ELISA - Human H-FABP ELISA Kit (AB243682)

Sandwich ELISA - Human H-FABP ELISA Kit (AB243682)

Sandwich ELISA - Human H-FABP ELISA Kit (AB243682)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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Product promise