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AB326008

Human H-FABP ELISA Kit, Chemiluminescent

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Human H-FABP ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human H-FABP with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 1.92 - 4200 pg/ml with a sensitivity of 0.76 pg/mL.
- Validated on a number of sample types including cerebrospinal fluid (CSF).
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

FABP11, MDGI, FABP3, Fatty acid-binding protein 3, Heart-type fatty acid-binding protein, Mammary-derived growth inhibitor, Muscle fatty acid-binding protein, H-FABP, M-FABP

7 Images
Sandwich ELISA - Human H-FABP ELISA Kit, Chemiluminescent (AB326008)
  • sELISA

Lab

Sandwich ELISA - Human H-FABP ELISA Kit, Chemiluminescent (AB326008)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human H-FABP ELISA Kit, Chemiluminescent (AB326008)
  • sELISA

Lab

Sandwich ELISA - Human H-FABP ELISA Kit, Chemiluminescent (AB326008)

Interpolated concentrations of native H-FABP in human heart extract based on a 13 ng/mL extract load. The concentrations of H-FABP were measured in duplicate and interpolated from the H-FABP standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human H-FABP ELISA Kit, Chemiluminescent (AB326008)
  • sELISA

Lab

Sandwich ELISA - Human H-FABP ELISA Kit, Chemiluminescent (AB326008)

Dynamic range comparison between the Chemiluminescent Human H-FABP SimpleStep ELISA, colorimetric Human H-FABP SimpleStep ELISA (ab243682), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human H-FABP ELISA Kit, Chemiluminescent (AB326008)
  • sELISA

Lab

Sandwich ELISA - Human H-FABP ELISA Kit, Chemiluminescent (AB326008)

Standard curve comparison between the Chemiluminescent Human H-FABP SimpleStep ELISA and colorimetric Human H-FABP SimpleStep ELISA (ab243682) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human H-FABP ELISA Kit, Chemiluminescent (AB326008)
  • sELISA

Lab

Sandwich ELISA - Human H-FABP ELISA Kit, Chemiluminescent (AB326008)

Serum from eight individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 1,635.8 pg/mL with a range of 873 – 2,940 pg/mL.

Sandwich ELISA - Human H-FABP ELISA Kit, Chemiluminescent (AB326008)
  • sELISA

Lab

Sandwich ELISA - Human H-FABP ELISA Kit, Chemiluminescent (AB326008)

Interpolated concentrations of native H-FABP in human milk sample. The concentrations of H-FABP were measured in duplicates, interpolated from the H-FABP standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 1 : 50,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 4,273 ng/mL in milk.

Sandwich ELISA - Human H-FABP ELISA Kit, Chemiluminescent (AB326008)
  • sELISA

Lab

Sandwich ELISA - Human H-FABP ELISA Kit, Chemiluminescent (AB326008)

The concentrations of H-FABP were measured in duplicates, interpolated from the H-FABP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (heparin) 25%, cerebrospinal fluid 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 1.93 ng/mL in serum, 2.05 ng/mL in plasma (citrate), 1.57 ng/mL in plasma (heparin), and 1.3 ng/mL in cerebrospinal fluid.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, Heparin Plasma, Milk, Cerebral Spinal Fluid, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.76 pg/mL

Range

1.92 - 4200 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human H-FABP ELISA Kit, Chemiluminescent (ab326008) is a rapid single-wash sandwich 55-min ELISA to measure Human H-FABP in Serum, Citrate Plasma, Heparin Plasma, Milk, Cerebrospinal Fluid, Tissue Extract with a sensitivity of 0.76 pg/mL.

How the assay works

Human H-FABP ELISA Kit, Chemiluminescent (ab326008) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human H-FABP (ab326008) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-H-FABP antibody [EPR21935-204] - BSA and Aside free (Capture) ab259521
- Anti-H-FABP antibody [EPR21935-201] - BSA and Aside free (Detector) ab259522

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 2, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 12.4" } ]
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Recovery

[ { "sample": "Serum", "range": "82 - 90 %", "average": "= 86" }, { "sample": "Citrate plasma", "range": "95 - 103 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "89 - 94 %", "average": "= 91" }, { "sample": "Cerebral Spinal Fluid", "range": "107 - 113 %", "average": "= 110" }, { "sample": "Milk", "range": "82 - 94 %", "average": "= 87" }, { "sample": "Tissue Extracts", "range": "104 - 108 %", "average": "= 106" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326008-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Human H-FABP Lyophilized Recombinant Protein", "number":"AB326008-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human H-FABP Detector Antibody", "number":"AB326008-CMP02", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326008-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB326008-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB326008-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326008-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB326008-CMP11", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326008-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human H-FABP Capture Antibody", "number":"AB326008-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326008-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326008-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

FABPs are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
See full target information FABP3
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com