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AB267639

Human HAI-1 ELISA Kit

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Human HAI-1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human HAI-1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

HAI1, UNQ223/PRO256, SPINT1, Kunitz-type protease inhibitor 1, Hepatocyte growth factor activator inhibitor type 1, HAI-1

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Sandwich ELISA - Human HAI-1 ELISA Kit (AB267639)
  • sELISA

Supplier Data

Sandwich ELISA - Human HAI-1 ELISA Kit (AB267639)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.8 ng/mL

Range

0.819 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human HAI-1 ELISA Kit is designed for the quantitative determination of HAI-1 in cell culture supernatants, plasma and serum samples.

Δ Note: Human HAI-1 concentration is low in normal serum and plasma and may not be detectable in this assay.

This assay employs an antibody specific for human HAI-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and HAI-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human HAI-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HAI-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

No cross-reactivity with mouse HAI-1, human APP, GASP-1, GASP-2, HAI-2A, TFPI, TFPI-2 is observed.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "68 - 82 %", "average": "= 72.47" }, { "sample": "Plasma", "range": "69 - 150 %", "average": "= 100" }, { "sample": "Cell culture media", "range": "67 - 83 %", "average": "= 72.35" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267639-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267639-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267639-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human HAI-1 coated Microplate (12 x 8 wells)", "number":"AB267639-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB267639-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB267639-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267639-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human HAI-1 Standard (Lyophilized)", "number":"AB267639-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB267639-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human HAI-1 Detection Antibody", "number":"AB267639-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HAI-1 known also as Hepatocyte Growth Factor Activator Inhibitor 1 or SPINT1 is a type 1 transmembrane protein with a molecular mass of around 55 kDa. This protein commonly expresses in epithelial cells particularly in tissues like the stomach and liver. It serves as a serine protease inhibitor playing a role in regulating the activity of certain proteases. Mouse HAI protein comparable in function and structure is often used in research as a model for understanding HAI-1 in humans. Researchers frequently use HAI detectors and HAI-1 ELISA methods for precise quantification and analysis.
Biological function summary

HAI-1 performs a regulatory role by inhibiting proteases such as matriptase and hepsin which are critical for many cellular functions. As part of a complex HAI-1 binds to these proteases controlling their activity to prevent excessive proteolysis which can damage tissues. This regulatory function is key in maintaining epithelial integrity and homeostasis impacting processes like cell growth and migration. Anti-HAI studies show that disrupting this balance can lead to a cascade of biological alterations.

Pathways

Several cellular processes heavily depend on the balance maintained by HAI-1 in protease activity. One significant pathway involves the HGF/c-Met signaling cascade which impacts cell motility and proliferation. By inhibiting matriptase HAI-1 indirectly affects this pathway regulating cellular responses. Additionally HAI-1 also relates to epithelial-mesenchymal transition (EMT) through controlling proteolytic activity which can influence how cells migrate and differentiate in development or healing.

Deregulation of HAI-1 often links to cancer progression specifically in cases like breast or prostate cancer. In these contexts improper regulation of proteases can lead to enhanced tumor invasiveness and metastasis. HAI-1's interaction with the c-Met receptor pathway facilitated by HGFA illustrates its significant role in cancer biology. Disorders of the liver such as fibrosis also reveal connections where HAI-1 modulation of protease activity affects extracellular matrix turnover implicating HAI-1 in the fibrogenic response alongside proteins like matriptase.
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Product protocols

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Target data

Inhibitor of HGFAC (PubMed : 9045658). Inhibits serine protease activity of ST14/matriptase in vitro (PubMed : 28710277). Inhibits serine protease activity of TMPRSS13, via the BPTI/Kunitz inhibitor 1 domain (PubMed : 20977675).
See full target information SPINT1
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websiteProtocolBooklet
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