AB288176

Human HAI-1 ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Human HAI-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human HAI-1 with a sensitivity of 0.29 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

HAI1, UNQ223/PRO256, SPINT1, Kunitz-type protease inhibitor 1, Hepatocyte growth factor activator inhibitor type 1, HAI-1

4 Images

Sandwich ELISA - Human HAI-1 ELISA Kit (AB288176)

sELISA

Supplier Data

Sandwich ELISA - Human HAI-1 ELISA Kit (AB288176)

Interpolated concentrations of human HAI-1 in PBMC supernatant stimulated for 46 hours with 1.5% PHA-M. Interpolated concentration of native HAI-1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : stimulated PBMC supernatant (100%). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human HAI-1 ELISA Kit (AB288176)

Interpolated concentrations of HAI-1 in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean HAI-1 concentration was determined to be 93.19 ng/mL with a range of 54.39 - 161.95 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human HAI-1 ELISA Kit (AB288176)

Interpolated concentrations of human HAI-1 in serum, plasma (citrate), plasma (heparin), and plasma (EDTA). Interpolated concentration of native HAI-1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 25%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human HAI-1 ELISA Kit (AB288176)

Example of human HAI-1 standard curve in Sample Diluent NS. Example of human HAI-1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.29 ng/mL

Range

1.094 - 70 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human HAI-1 ELISA Kit (ab288176) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human HAI-1 protein in cell culture supernatant, plasma and serum samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human HAI-1 with 0.29pg/mL sensitivity

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.8" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "83 - 96 %", "average": "= 89" }, { "sample": "Serum", "range": "80 - 91 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "82 - 117 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "86 - 94 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "85 - 102 %", "average": "= 96" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HAI-1 known also as Hepatocyte Growth Factor Activator Inhibitor 1 or SPINT1 is a type 1 transmembrane protein with a molecular mass of around 55 kDa. This protein commonly expresses in epithelial cells particularly in tissues like the stomach and liver. It serves as a serine protease inhibitor playing a role in regulating the activity of certain proteases. Mouse HAI protein comparable in function and structure is often used in research as a model for understanding HAI-1 in humans. Researchers frequently use HAI detectors and HAI-1 ELISA methods for precise quantification and analysis.

Biological function summary

HAI-1 performs a regulatory role by inhibiting proteases such as matriptase and hepsin which are critical for many cellular functions. As part of a complex HAI-1 binds to these proteases controlling their activity to prevent excessive proteolysis which can damage tissues. This regulatory function is key in maintaining epithelial integrity and homeostasis impacting processes like cell growth and migration. Anti-HAI studies show that disrupting this balance can lead to a cascade of biological alterations.

Pathways

Several cellular processes heavily depend on the balance maintained by HAI-1 in protease activity. One significant pathway involves the HGF/c-Met signaling cascade which impacts cell motility and proliferation. By inhibiting matriptase HAI-1 indirectly affects this pathway regulating cellular responses. Additionally HAI-1 also relates to epithelial-mesenchymal transition (EMT) through controlling proteolytic activity which can influence how cells migrate and differentiate in development or healing.

Deregulation of HAI-1 often links to cancer progression specifically in cases like breast or prostate cancer. In these contexts improper regulation of proteases can lead to enhanced tumor invasiveness and metastasis. HAI-1's interaction with the c-Met receptor pathway facilitated by HGFA illustrates its significant role in cancer biology. Disorders of the liver such as fibrosis also reveal connections where HAI-1 modulation of protease activity affects extracellular matrix turnover implicating HAI-1 in the fibrogenic response alongside proteins like matriptase.

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Product protocols

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Target data

Inhibitor of HGFAC (PubMed : 9045658). Inhibits serine protease activity of ST14/matriptase in vitro (PubMed : 28710277). Inhibits serine protease activity of TMPRSS13, via the BPTI/Kunitz inhibitor 1 domain (PubMed : 20977675).

See full target information SPINT1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e21616 PubMed38027820

2023

Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyan Chen,Jingyang Li,Yilan You,Zhiwen Qian,Jiayu Liu,Ying Jiang,Ying Gu,Jianping Xiao,Yan Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human HAI-1 ELISA Kit

Sandwich ELISA - Human HAI-1 ELISA Kit (AB288176)

Sandwich ELISA - Human HAI-1 ELISA Kit (AB288176)

Sandwich ELISA - Human HAI-1 ELISA Kit (AB288176)

Sandwich ELISA - Human HAI-1 ELISA Kit (AB288176)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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