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AB309417

Human HAO1/GOX ELISA Kit

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Human HAO1/GOX ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human HAO1/GOX in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

GOX1, HAO1, 2-Hydroxyacid oxidase 1, HAOX1, Glycolate oxidase, Glyoxylate oxidase, GO, GOX

1 Images
Sandwich ELISA - Human HAO1/GOX ELISA Kit (AB309417)
  • sELISA

Supplier Data

Sandwich ELISA - Human HAO1/GOX ELISA Kit (AB309417)

Example of standard curve using ab309417. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.17 ng/mL

Range

0.17 - 50 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human HAO1/GOX ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD42b in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human HAO1/GOX coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "76 - 135 %", "average": "= 105" }, { "sample": "Plasma", "range": "119 - 139 %", "average": "= 129.2" }, { "sample": "Cell culture supernatant", "range": "74 - 122 %", "average": "= 91.13" }, { "sample": "Cell culture media", "range": "74 - 122 %", "average": "= 91.13" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The enzyme HAO1 also known as GOX (glycolate oxidase) functions by catalyzing the oxidation of glycolate to glyoxylate generating hydrogen peroxide in peroxisomes. The molecular mass of HAO1 is approximately 43 kDa. It is expressed in several tissues with high levels found in liver and kidney. The protein may also be referred to as alpha GOX which highlights its identity and specialization within oxidase family.
Biological function summary

HAO1 facilitates the metabolism of hydroxyl acids playing an important role in glyoxylate and dicarboxylate metabolism. It does not form part of a multi-protein complex but operates as a monomer maintaining cellular homeostasis of organic acids. By helping convert glycolate into a form that cells use or excrete HAO1 prevents the accumulation of compounds that can become toxic at elevated levels.

Pathways

Glycolate oxidase takes part in the glyoxylate cycle and photorespiration in plants which also has parallels in animal metabolism where it participates in handling waste products. HAO1 interacts within the network of metabolic pathways along with proteins like lactate dehydrogenase that facilitate the conversion of chemical compounds. It plays a role in broader metabolomic balancing acts that maintain overall organismal homeostasis.

Mutations and dysfunctions in HAO1 have links to primary hyperoxaluria type 1 a disease characterized by excessive production and build-up of oxalate leading to kidney stones and renal failure. The interplay with proteins like alanine:glyoxylate aminotransferase which helps detoxify glyoxylate is important in these conditions. This disorder highlights the importance of proper glycolate metabolism and its regulation in maintaining health.
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Product protocols

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Target data

Broad substrate specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate (PubMed : 10777549, PubMed : 10978532, PubMed : 17669354, PubMed : 18215067). The glyoxylate produced by the oxidation of glycolate can then be utilized by alanine-glyoxylate aminotransferase for the peroxisomal synthesis of glycine; this pathway appears to be an important step for the detoxification of glyoxylate which, if allowed to accumulate, may be metabolized to oxalate with formation of kidney stones (PubMed : 10978532, PubMed : 17669354). Can also catalyze the oxidation of glyoxylate, and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate, albeit with much lower catalytic efficiency (PubMed : 10777549, PubMed : 17669354, PubMed : 18215067). Active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), but O2 is believed to be the physiological electron acceptor, leading to the production of H2O2 (PubMed : 10777549, PubMed : 10978532, PubMed : 17669354, PubMed : 18215067). Is not active on L-lactate and 2-hydroxybutanoate (PubMed : 10777549).
See full target information HAO1
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websiteProtocolBooklet
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Complementary Products

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AB194790

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Western blot - Anti-HAO1/GOX antibody (AB194790)

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Proteins & Peptides

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Cell Lines & Lysates

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Sanger Sequencing - Human HAO1 (GOX) knockout HeLa cell line (AB264716)

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Cell Lines & Lysates

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