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AB309407

Human HAPLN1 ELISA Kit

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Human HAPLN1 ELISA Kit is a Sandwich ELISA for the measurement of Human HAPLN1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CRTL1, HAPLN1, Hyaluronan and proteoglycan link protein 1, Cartilage-linking protein 1, Proteoglycan link protein, Cartilage-link protein

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Sandwich ELISA - Human HAPLN1 ELISA Kit (AB309407)
  • sELISA

Supplier Data

Sandwich ELISA - Human HAPLN1 ELISA Kit (AB309407)

Example of standard curve using ab309407. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.75 ng/mL

Range

0.75 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB181997

Anti-HAPLN1 antibody [EPR6338]

RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-HAPLN1 antibody [EPR6338] (AB181997)

  • 3
  • 3
Proteins & Peptides

AB276245

Recombinant Human HAPLN1 protein (His tag)

SDS-PAGE - Recombinant Human HAPLN1 protein (His tag) (AB276245)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human HAPLN1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD42b in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human HAPLN1 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "93 - 119 %", "average": "= 105.6" }, { "sample": "Plasma", "range": "79 - 100 %", "average": "= 88.85" }, { "sample": "Cell culture supernatant", "range": "90 - 117 %", "average": "= 102" }, { "sample": "Cell culture media", "range": "90 - 117 %", "average": "= 102" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309407-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Human HAPLN1 Antibody-coated ELISA Plate", "number":"AB309407-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309407-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB309407-CMP12", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309407-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human HAPLN1 Detection Antibody", "number":"AB309407-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human HAPLN1 Protein Standard", "number":"AB309407-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309407-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309407-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HAPLN1 also known as hyaluronan and proteoglycan link protein 1 is an important structural component in the extracellular matrix. This protein links hyaluronan to proteoglycans which contributes to the integrity and function of the matrix. HAPLN1 has a molecular mass of approximately 39 kDa and is expressed in various tissues including cartilage and nervous tissues. Its expression plays a significant role in maintaining the properties of these tissues.
Biological function summary

Hyaluronan and proteoglycan link protein 1 supports the organization and stabilization of the extracellular matrix network. As part of this framework HAPLN1 forms complexes with hyaluronan and aggrecan ensuring structural stability and resilience in tissues experiencing mechanical stress. The association with such complexes impacts cellular signaling and tissue hydration which are important for maintaining tissue homeostasis and function.

Pathways

HAPLN1 participates in extracellular matrix organization and cell signaling processes. It is actively involved in the aggrecan pathway which influences cartilage development and repair. HAPLN1 is also connected to proteins like aggrecan and link proteins that help mediate interactions within the matrix and regulate cellular responses to mechanical stress.

Abnormal expression or function of HAPLN1 has links to osteoarthritis and intervertebral disc degeneration. In these conditions the disruption of extracellular matrix composition alters tissue integrity and function. An imbalance involving HAPLN1 along with proteins like aggrecan contributes to the pathogenesis and progression of these disorders affecting joint and disc health.
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Product protocols

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Target data

Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix.
See full target information HAPLN1
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websiteProtocolBooklet
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Product promise

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