Human Haptoglobin ELISA Kit is a Competitive ELISA kit for the measurement of Human Haptoglobin in Human in Plasma, Serum samples.
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- Pulmonary circulation 13:e121852023Transpulmonary generation of cell-free hemoglobin contributes to vascular dysfunction in pulmonary arterial hypertension via dysregulated clearance mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJamie E Meegan,Vern Eric Kerchberger,Niki L Fortune,Joel Brennan McNeil,Julie A Bastarache,Eric D Austin,Lorraine B Ware,Anna R Hemnes,Evan L BrittainPubMed 36743426
- Analytical and bioanalytical chemistry 415:2045-20572022First bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCristina Muñoz-San Martín,Ana Montero-Calle,María Garranzo-Asensio,Maria Gamella,Víctor Pérez-Ginés,María Pedrero,José M Pingarrón,Rodrigo Barderas,Noemí de-Los-Santos-Álvarez,María Jesús Lobo-Castañón,Susana CampuzanoPubMed 36344668
- Experimental physiology 106:2198-22092021Sub-maximal aerobic exercise training reduces haematocrit and ameliorates symptoms in Andean highlanders with chronic mountain sickness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJosé Luis Macarlupú,Gustavo Vizcardo-Galindo,Rómulo Figueroa-Mujíca,Nicolas Voituron,Jean-Paul Richalet,Francisco C VillafuertePubMed 34555237
- Medicine 98:e174162019Clinical value of haptoglobin and soluble CD163 testing for the differential diagnosis of tuberculous and malignant pleural effusions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinying Gao,Lei Song,Dan Li,Liping Peng,Hui DingPubMed 31626097
- Functional & integrative genomics 20:191-2002019Plasma protein(s)-based conceptual diagnostic tool for assessing high-altitude acclimation in humans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSubhojit Paul,Anamika Gangwar,Kalpana Bhargava,Nilofar Khan,Pankaj Khurana,Yasmin AhmadPubMed 31444657
- Experimental and therapeutic medicine 18:1417-14252019Effect of haptoglobin on the treatment of chronic hepatitis B with interferon.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin-Ling Lv et. al.PubMed 31316628
- FASEB journal : official publication of the Federa 33:8221-82312019genotype influences the gut microbiome structure and function in humans and mice: relevance for Alzheimer's disease pathophysiology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTam T T Tran et. al.PubMed 30958695
- Medical science monitor : international medical jo 24:4146-41532018Application of Isobaric Tags for Relative and Absolute Quantification (iTRAQ) Coupled with Two-Dimensional Liquid Chromatography/Tandem Mass Spectrometry in Quantitative Proteomic Analysis for Discovery of Serum Biomarkers for Idiopathic Pulmonary Fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Zhang et. al.PubMed 29909421
- Kardiochirurgia i torakochirurgia polska = Polish 14:175-1792017Late subclinical hemolysis and long-term outcomes after aortic valve replacement with On-X mechanical prostheses - a preliminary single-center report.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBartłomiej Perek et. al.PubMed 29181045
- The International journal of angiology : official publication of the International College of Angiology, Inc 25:153-82016Investigation of the Etiology of Anemia in Thromboangiitis Obliterans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohammad Mehdi Akbarin,Hassan Ravari,Ataollah Rajabnejad,Narges Valizadeh,Bahare FazeliPubMed 27574381
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum
- Assay type
- Competitive
- Reactive species
- Human
- Range
- 0.078 - 20 µg/mL
- Assay time
- 2h
- Sensitivity
- = 57 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity, and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. The uncleaved form of allele alpha-2 (2-2), known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
Alternative names
Haptoglobin, Zonulin, HP
What's included?
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Human Haptoglobin ELISA Kit is a Competitive ELISA kit for the measurement of Human Haptoglobin in Human in Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum
- Assay type
- Competitive
- Reactive species
- Human
- Range
- 0.078 - 20 µg/mL
- Assay time
- 2h
- Assay Platform
- Microplate
- Sensitivity
- = 57 ng/mL
- General recovery
- 90%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 5.1 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 10.5 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Human Haptoglobin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Haptoglobin in plasma and serum.
A Haptoglobin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Haptoglobin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Haptoglobin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Haptoglobin (Hp) also known as Hp protein is a glycoprotein with a molecular weight ranging from 85 to 100 kDa varying due to its ability to form different dimeric and multimeric structures. It is primarily synthesized in the liver and circulating in the plasma. Mechanically haptoglobin binds free hemoglobin released from erythrocytes preventing oxidative damage and hemoglobin-driven deleterious effects. Haptoglobin's binding to hemoglobin forms a haptoglobin-hemoglobin complex that gets cleared by the CD163 receptor on macrophages mainly in the liver and spleen.
- Biological function summary
Haptoglobin functions as an antioxidant and an acute phase protein playing roles in inflammation and immune response. It is an essential component of the antioxidant defense system reducing oxidative stress by binding free hemoglobin. Additionally haptoglobin can participate in complex formation with hemoglobin to facilitate the clearance of excess free hemoglobin from circulation. Its expression increases during inflammation under the regulation of cytokines like IL-6.
- Pathways
The haptoglobin protein is part of the hemoglobin clearance pathway integral for iron metabolism and homeostasis. This pathway ensures the safe removal of hemoglobin from the blood helping to prevent kidney damage. Haptoglobin is related to other proteins such as CD163 and the liver-derived hepatocellular proteins involved in the clearance process. Also haptoglobin is linked to antioxidant pathways collaborating with oxidative stress regulators to manage reactive oxygen species levels.
- Associated diseases and disorders
Changes in haptoglobin levels are associated with hemolytic anemia and inflammatory conditions. Low haptoglobin levels often suggest hemolytic anemia due to its rapid binding with free hemoglobin in the bloodstream. During inflammation and infections 'anti-Hp' tests (haptoglobin tests) may show increased haptoglobin levels as a response to cytokine signaling. Moreover haptoglobin polymorphisms have a connection with ischemic stroke susceptibility where its isoforms show differing efficiencies in binding free hemoglobin. These findings highlight haptoglobin's relevance as a biomarker for these conditions and illustrate its interaction with hematologic and immune proteins in disease contexts.
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2 product images
Competitive ELISA - Human Haptoglobin ELISA Kit (ab108856)
Haptoglobin measured in various biological fluids showing quantity (μg) per mL of sample tested
Competitive ELISA - Human Haptoglobin ELISA Kit (ab108856)
Typical Standard Curve. Representative Standard Curve using ab108856.
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Product protocols
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