AB108856

Human Haptoglobin ELISA Kit

Name: Human Haptoglobin ELISA Kit (ab108856) | Abcam
Brand: Abcam Limited
SKU: AB108856

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(12 Publications)

Human Haptoglobin ELISA Kit is a Competitive ELISA for the measurement of Human Haptoglobin in Human in Biofluids samples.

View Alternative Names

Haptoglobin, Zonulin, HP

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Competitive ELISA - Human Haptoglobin ELISA Kit (AB108856)

cELISA

Supplier Data

Competitive ELISA - Human Haptoglobin ELISA Kit (AB108856)

Typical Standard Curve. Representative Standard Curve using ab108856.

cELISA

Lab

Competitive ELISA - Human Haptoglobin ELISA Kit (AB108856)

Haptoglobin measured in various biological fluids showing quantity (μg) per mL of sample tested

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Competitive

Sensitivity

= 57 ng/mL

Range

0.078 - 20 µg/mL

Assay time

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Haptoglobin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Haptoglobin in plasma and serum.

A Haptoglobin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Haptoglobin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Haptoglobin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.1" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Haptoglobin (Hp) also known as Hp protein is a glycoprotein with a molecular weight ranging from 85 to 100 kDa varying due to its ability to form different dimeric and multimeric structures. It is primarily synthesized in the liver and circulating in the plasma. Mechanically haptoglobin binds free hemoglobin released from erythrocytes preventing oxidative damage and hemoglobin-driven deleterious effects. Haptoglobin's binding to hemoglobin forms a haptoglobin-hemoglobin complex that gets cleared by the CD163 receptor on macrophages mainly in the liver and spleen.

Biological function summary

Haptoglobin functions as an antioxidant and an acute phase protein playing roles in inflammation and immune response. It is an essential component of the antioxidant defense system reducing oxidative stress by binding free hemoglobin. Additionally haptoglobin can participate in complex formation with hemoglobin to facilitate the clearance of excess free hemoglobin from circulation. Its expression increases during inflammation under the regulation of cytokines like IL-6.

Pathways

The haptoglobin protein is part of the hemoglobin clearance pathway integral for iron metabolism and homeostasis. This pathway ensures the safe removal of hemoglobin from the blood helping to prevent kidney damage. Haptoglobin is related to other proteins such as CD163 and the liver-derived hepatocellular proteins involved in the clearance process. Also haptoglobin is linked to antioxidant pathways collaborating with oxidative stress regulators to manage reactive oxygen species levels.

Changes in haptoglobin levels are associated with hemolytic anemia and inflammatory conditions. Low haptoglobin levels often suggest hemolytic anemia due to its rapid binding with free hemoglobin in the bloodstream. During inflammation and infections 'anti-Hp' tests (haptoglobin tests) may show increased haptoglobin levels as a response to cytokine signaling. Moreover haptoglobin polymorphisms have a connection with ischemic stroke susceptibility where its isoforms show differing efficiencies in binding free hemoglobin. These findings highlight haptoglobin's relevance as a biomarker for these conditions and illustrate its interaction with hematologic and immune proteins in disease contexts.

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Product protocols

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Target data

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity, and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.. The uncleaved form of allele alpha-2 (2-2), known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.

See full target information HP

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Pulmonary circulation 13:e12185 PubMed36743426

2023

Transpulmonary generation of cell-free hemoglobin contributes to vascular dysfunction in pulmonary arterial hypertension via dysregulated clearance mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Jamie E Meegan,Vern Eric Kerchberger,Niki L Fortune,Joel Brennan McNeil,Julie A Bastarache,Eric D Austin,Lorraine B Ware,Anna R Hemnes,Evan L Brittain

Analytical and bioanalytical chemistry 415:2045-2057 PubMed36344668

2022

First bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Muñoz-San Martín,Ana Montero-Calle,María Garranzo-Asensio,Maria Gamella,Víctor Pérez-Ginés,María Pedrero,José M Pingarrón,Rodrigo Barderas,Noemí de-Los-Santos-Álvarez,María Jesús Lobo-Castañón,Susana Campuzano

Experimental physiology 106:2198-2209 PubMed34555237

2021

Sub-maximal aerobic exercise training reduces haematocrit and ameliorates symptoms in Andean highlanders with chronic mountain sickness.

Applications

Unspecified application

Species

Unspecified reactive species

José Luis Macarlupú,Gustavo Vizcardo-Galindo,Rómulo Figueroa-Mujíca,Nicolas Voituron,Jean-Paul Richalet,Francisco C Villafuerte

Medicine 98:e17416 PubMed31626097

2019

Clinical value of haptoglobin and soluble CD163 testing for the differential diagnosis of tuberculous and malignant pleural effusions.

Applications

Unspecified application

Species

Unspecified reactive species

Jinying Gao,Lei Song,Dan Li,Liping Peng,Hui Ding

Functional & integrative genomics 20:191-200 PubMed31444657

2019

Plasma protein(s)-based conceptual diagnostic tool for assessing high-altitude acclimation in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Subhojit Paul,Anamika Gangwar,Kalpana Bhargava,Nilofar Khan,Pankaj Khurana,Yasmin Ahmad

Experimental and therapeutic medicine 18:1417-1425 PubMed31316628

2019

Effect of haptoglobin on the treatment of chronic hepatitis B with interferon.

Applications

Unspecified application

Species

Unspecified reactive species

Lin-Ling Lv,Yi-Xuan Yang,Tong-Dong Shi

FASEB journal : official publication of the Federa 33:8221-8231 PubMed30958695

2019

genotype influences the gut microbiome structure and function in humans and mice: relevance for Alzheimer's disease pathophysiology.

Applications

Unspecified application

Species

Unspecified reactive species

Tam T T Tran,Simone Corsini,Lee Kellingray,Claire Hegarty,Gwénaëlle Le Gall,Arjan Narbad,Michael Müller,Noemi Tejera,Paul W O'Toole,Anne-Marie Minihane,David Vauzour

Medical science monitor : international medical jo 24:4146-4153 PubMed29909421

2018

Application of Isobaric Tags for Relative and Absolute Quantification (iTRAQ) Coupled with Two-Dimensional Liquid Chromatography/Tandem Mass Spectrometry in Quantitative Proteomic Analysis for Discovery of Serum Biomarkers for Idiopathic Pulmonary Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhang,Qian Xin,Zhen Wu,Chaochao Wang,Yongbin Wang,Qian Wu,Rui Niu

Kardiochirurgia i torakochirurgia polska = Polish 14:175-179 PubMed29181045

2017

Late subclinical hemolysis and long-term outcomes after aortic valve replacement with On-X mechanical prostheses - a preliminary single-center report.

Applications

Unspecified application

Species

Unspecified reactive species

Bartłomiej Perek,Sylwia Sławek,Agnieszka Malińska,Izabela Katyńska,Mateusz Puślecki,Bogumiła Szymak-Pawełczyk,Michał Nowicki,Marek Jemielity

The International journal of angiology : official publication of the International College of Angiology, Inc 25:153-8 PubMed27574381

2016

Investigation of the Etiology of Anemia in Thromboangiitis Obliterans.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Mehdi Akbarin,Hassan Ravari,Ataollah Rajabnejad,Narges Valizadeh,Bahare Fazeli

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human Haptoglobin ELISA Kit

Competitive ELISA - Human Haptoglobin ELISA Kit (AB108856)

Competitive ELISA - Human Haptoglobin ELISA Kit (AB108856)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Proteins & Peptides

Assay Kits

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise