AB108858

Human Haptoglobin ELISA Kit

Name: Human Haptoglobin ELISA Kit (ab108858) | Abcam
Brand: Abcam Limited
SKU: AB108858

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(3 Publications)

Human Haptoglobin ELISA Kit is a Sandwich ELISA for the measurement of Human Haptoglobin in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Haptoglobin, Zonulin, HP

1 Images

Sandwich ELISA - Human Haptoglobin ELISA Kit (AB108858)

sELISA

Supplier Data

Sandwich ELISA - Human Haptoglobin ELISA Kit (AB108858)

Representative Standard Curve using ab108858

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Cerebral Spinal Fluid, Milk, Saliva, Urine

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.1 ng/mL

Range

3.125 - 200 ng/mL

Assay time

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's Haptoglobin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Haptoglobin concentrations in Human urine, saliva, milk and cell culture supernatants.

A Haptoglobin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Haptoglobin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Haptoglobin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108858-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108858-CMP05", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108858-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Chromogen Substrate", "number":"AB108858-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Haptoglobin Standard", "number":"AB108858-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB108858-CMP07", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108858-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Haptoglobin Microplate (12 x 8 well strips)", "number":"AB108858-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108858-CMP03", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Haptoglobin Antibody", "number":"AB108858-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Haptoglobin (Hp) also known as Hp protein is a glycoprotein with a molecular weight ranging from 85 to 100 kDa varying due to its ability to form different dimeric and multimeric structures. It is primarily synthesized in the liver and circulating in the plasma. Mechanically haptoglobin binds free hemoglobin released from erythrocytes preventing oxidative damage and hemoglobin-driven deleterious effects. Haptoglobin's binding to hemoglobin forms a haptoglobin-hemoglobin complex that gets cleared by the CD163 receptor on macrophages mainly in the liver and spleen.

Biological function summary

Haptoglobin functions as an antioxidant and an acute phase protein playing roles in inflammation and immune response. It is an essential component of the antioxidant defense system reducing oxidative stress by binding free hemoglobin. Additionally haptoglobin can participate in complex formation with hemoglobin to facilitate the clearance of excess free hemoglobin from circulation. Its expression increases during inflammation under the regulation of cytokines like IL-6.

Pathways

The haptoglobin protein is part of the hemoglobin clearance pathway integral for iron metabolism and homeostasis. This pathway ensures the safe removal of hemoglobin from the blood helping to prevent kidney damage. Haptoglobin is related to other proteins such as CD163 and the liver-derived hepatocellular proteins involved in the clearance process. Also haptoglobin is linked to antioxidant pathways collaborating with oxidative stress regulators to manage reactive oxygen species levels.

Changes in haptoglobin levels are associated with hemolytic anemia and inflammatory conditions. Low haptoglobin levels often suggest hemolytic anemia due to its rapid binding with free hemoglobin in the bloodstream. During inflammation and infections 'anti-Hp' tests (haptoglobin tests) may show increased haptoglobin levels as a response to cytokine signaling. Moreover haptoglobin polymorphisms have a connection with ischemic stroke susceptibility where its isoforms show differing efficiencies in binding free hemoglobin. These findings highlight haptoglobin's relevance as a biomarker for these conditions and illustrate its interaction with hematologic and immune proteins in disease contexts.

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Product protocols

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Target data

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity, and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.. The uncleaved form of allele alpha-2 (2-2), known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.

See full target information HP

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neurology 13:886040 PubMed36003300

2022

Identification of protein biomarkers in host cerebrospinal fluid for differential diagnosis of tuberculous meningitis and other meningitis.

Applications

Unspecified application

Species

Unspecified reactive species

Mailing Huang,Zeyu Ding,Wensheng Li,Weibi Chen,Yadong Du,Hongyan Jia,Qi Sun,Boping Du,Rongrong Wei,Aiying Xing,Qi Li,Naihui Chu,Liping Pan

PLoS medicine 15:e1002522 PubMed29522519

2018

Role of heme in lung bacterial infection after trauma hemorrhage and stored red blood cell transfusion: A preclinical experimental study.

Applications

Unspecified application

Species

Unspecified reactive species

Brant M Wagener,Parker J Hu,Joo-Yeun Oh,Cilina A Evans,Jillian R Richter,Jaideep Honavar,Angela P Brandon,Judy Creighton,Shannon W Stephens,Charity Morgan,Randal O Dull,Marisa B Marques,Jeffrey D Kerby,Jean-Francois Pittet,Rakesh P Patel

Redox biology 9:167-177 PubMed27566280

2016

Absorbance and redox based approaches for measuring free heme and free hemoglobin in biological matrices.

Applications

Unspecified application

Species

Unspecified reactive species

Joo-Yeun Oh,Jennifer Hamm,Xin Xu,Kristopher Genschmer,Ming Zhong,Jeffrey Lebensburger,Marisa B Marques,Jeffrey D Kerby,Jean-Francois Pittet,Amit Gaggar,Rakesh P Patel

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Haptoglobin ELISA Kit

Sandwich ELISA - Human Haptoglobin ELISA Kit (AB108858)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Proteins & Peptides

Assay Kits

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise