AB100531

Human HB EGF ELISA Kit

Name: Human HB EGF ELISA Kit (ab100531) | Abcam
Brand: Abcam Limited
SKU: AB100531

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(3 Publications)

Human HB EGF ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human HB EGF in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

DTR, DTS, HEGFL, HBEGF, Proheparin-binding EGF-like growth factor

2 Images

Sandwich ELISA - Human HB EGF ELISA Kit (AB100531)

sELISA

Unknown

Sandwich ELISA - Human HB EGF ELISA Kit (AB100531)

Representative standard curve using ab100531

sELISA

Unknown

Sandwich ELISA - Human HB EGF ELISA Kit (AB100531)

Representative standard curve using ab100531

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 20 pg/mL

Range

16.38 - 4000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's HB EGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HB EGF in plasma and cell culture supernatants. (Human HB EGF concentration is pretty low in normal plasma, it may not be detected in this assay).

This assay employs an antibody specific for Human HB EGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HB EGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HB EGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HB EGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect the endogenous Human HB-EGF in normal serum with ab100531, only in serum spiked with Human HB-EGF.

Optimization may be required with urine samples.

The standard is affinity purified.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "73 - 90 %", "average": "= 81.22" }, { "sample": "Plasma", "range": "84 - 103 %", "average": "= 93.73" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100531-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Recombinant Human HB EGF Standard (lyophilized)", "number":"AB100531-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB100531-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB100531-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100531-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human HB EGF", "number":"AB100531-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB100531-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"HB EGF Microplate (12 x 8 wells)", "number":"AB100531-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100531-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100531-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100531-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HB-EGF also known as heparin-binding EGF-like growth factor is a member of the EGF family with an approximate molecular mass of 19-23 kDa. It is a membrane-anchored growth factor that can be cleaved to release a soluble form. HB-EGF expresses in a wide range of tissues including the heart kidneys lungs and the brain. As a growth factor its primary mechanical function involves binding to EGFR (epidermal growth factor receptor) and ERBB4 receptors stimulating cellular proliferation and survival.

Biological function summary

The actions of HB-EGF extend beyond simple growth factor roles. It participates as a modulator in wound healing processes cardiac development and neural tissue maintenance. The protein often functions as part of a signaling complex that involves other growth factors and receptors. Its engagement in biological processes links directly to modifying cellular behavior such as enhancing cell migration and inducing tissue repair mechanisms.

Pathways

HB-EGF fits into critical signaling routes such as the MAPK/ERK and PI3K/Akt pathways. These pathways are important in mediating cell growth survival and differentiation. HB-EGF associates with other proteins in these pathways such as Ras and Akt to facilitate downstream signaling that regulates cellular responses to environmental stimuli. The proper function of these pathways is essential for maintaining cellular homeostasis.

HB-EGF has strong associations with cancer and cardiovascular diseases. In various cancer types overexpression or dysregulation of HB-EGF contributes to tumorigenesis and progression through its interaction with EGFR promoting uncontrolled cellular proliferation. Similarly in cardiovascular diseases HB-EGF influences cardiac hypertrophy and heart failure by interacting with associated proteins such as MMPs (matrix metalloproteinases) which further modulate cardiac remodeling and dysfunction. Understanding these relationships helps in therapeutic targeting for related ailments.

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Product protocols

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Target data

Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

See full target information HBEGF

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 44:68 PubMed39994761

2025

Reciprocal regulation of MMP-28 and EGFR is required for sustaining proliferative signaling in PDAC.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengtao Hong,Xing Huang,Linghao Xia,Tingbo Liang,Xueli Bai

PLoS pathogens 12:e1005926 PubMed27711202

2016

Sphingosine 1-Phosphate Activation of EGFR As a Novel Target for Meningitic Escherichia coli Penetration of the Blood-Brain Barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangru Wang,Ravi Maruvada,Andrew J Morris,Jun O Liu,Michael J Wolfgang,Dong Jae Baek,Robert Bittman,Kwang Sik Kim

Oncotarget 6:22348-60 PubMed26084293

2015

ERBB activation modulates sensitivity to MEK1/2 inhibition in a subset of driver-negative melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine E Hutchinson,Douglas B Johnson,Adam S Johnson,Violeta Sanchez,Maria Kuba,Pengcheng Lu,Xi Chen,Mark C Kelley,Qingguo Wang,Zhongming Zhao,Mark Kris,Michael F Berger,Jeffrey A Sosman,William Pao

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human HB EGF ELISA Kit

Sandwich ELISA - Human HB EGF ELISA Kit (AB100531)

Sandwich ELISA - Human HB EGF ELISA Kit (AB100531)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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