Human HB EGF ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human HB EGF in Human in Plasma, Cell culture supernatant samples.
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- PLoS pathogens 12:e10059262016Sphingosine 1-Phosphate Activation of EGFR As a Novel Target for Meningitic Escherichia coli Penetration of the Blood-Brain Barrier.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangru Wang et. al.PubMed 27711202
- Oncotarget 6:22348-602015ERBB activation modulates sensitivity to MEK1/2 inhibition in a subset of driver-negative melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatherine E Hutchinson,Douglas B Johnson,Adam S Johnson,Violeta Sanchez,Maria Kuba,Pengcheng Lu,Xi Chen,Mark C Kelley,Qingguo Wang,Zhongming Zhao,Mark Kris,Michael F Berger,Jeffrey A Sosman,William PaoPubMed 26084293
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 16.38 - 4000 pg/mL
- Sensitivity
- < 20 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
Alternative names
DTR, DTS, HEGFL, HBEGF, Proheparin-binding EGF-like growth factor
What's included?
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Human HB EGF ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human HB EGF in Human in Plasma, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 16.38 - 4000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 20 pg/mL
- General recovery
- 90%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 81.22 | Range 73 - 90 % |
Sample type Plasma | Average % = 93.73 | Range 84 - 103 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's HB EGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HB EGF in plasma and cell culture supernatants. (Human HB EGF concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human HB EGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HB EGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HB EGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HB EGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Human HB-EGF in normal serum with ab100531, only in serum spiked with Human HB-EGF.
Optimization may be required with urine samples.
The standard is affinity purified.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HB-EGF also known as heparin-binding EGF-like growth factor is a member of the EGF family with an approximate molecular mass of 19-23 kDa. It is a membrane-anchored growth factor that can be cleaved to release a soluble form. HB-EGF expresses in a wide range of tissues including the heart kidneys lungs and the brain. As a growth factor its primary mechanical function involves binding to EGFR (epidermal growth factor receptor) and ERBB4 receptors stimulating cellular proliferation and survival.
- Biological function summary
The actions of HB-EGF extend beyond simple growth factor roles. It participates as a modulator in wound healing processes cardiac development and neural tissue maintenance. The protein often functions as part of a signaling complex that involves other growth factors and receptors. Its engagement in biological processes links directly to modifying cellular behavior such as enhancing cell migration and inducing tissue repair mechanisms.
- Pathways
HB-EGF fits into critical signaling routes such as the MAPK/ERK and PI3K/Akt pathways. These pathways are important in mediating cell growth survival and differentiation. HB-EGF associates with other proteins in these pathways such as Ras and Akt to facilitate downstream signaling that regulates cellular responses to environmental stimuli. The proper function of these pathways is essential for maintaining cellular homeostasis.
- Associated diseases and disorders
HB-EGF has strong associations with cancer and cardiovascular diseases. In various cancer types overexpression or dysregulation of HB-EGF contributes to tumorigenesis and progression through its interaction with EGFR promoting uncontrolled cellular proliferation. Similarly in cardiovascular diseases HB-EGF influences cardiac hypertrophy and heart failure by interacting with associated proteins such as MMPs (matrix metalloproteinases) which further modulate cardiac remodeling and dysfunction. Understanding these relationships helps in therapeutic targeting for related ailments.
Product promise
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Full details and terms and conditions can be found here:
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2 product images
Sandwich ELISA - Human HB EGF ELISA Kit (ab100531)
Representative standard curve using ab100531
Sandwich ELISA - Human HB EGF ELISA Kit (ab100531)
Representative standard curve using ab100531
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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