AB235654

Human HBB ELISA Kit

Recombinant
SimpleStep
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(3 Publications)

Human HBB ELISA Kit is a single-wash 90-min Simplestep used to quantify Human HBB with a sensitivity of 34.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

HBA2, HBA1, Hemoglobin subunit alpha, Alpha-globin, Hemoglobin alpha chain

3 Images

Sandwich ELISA - Human HBB ELISA Kit (AB235654)

sELISA

Supplier Data

Sandwich ELISA - Human HBB ELISA Kit (AB235654)

Interpolated concentrations of native HBB in human serum and plasma samples.

The concentrations of HBB were measured in duplicates, interpolated from the HBB standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (heparin) 5%, plasma (EDTA) 25% and plasma (citrate) 3.13% The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HBB concentration was determined to be 64.9 ng/mL in serum, 28.6 ng/mL in plasma (heparin), 27.1 ng/mL in plasma (EDTA) and 192.2 ng/mL in plasma (citrate).

sELISA

Supplier Data

Sandwich ELISA - Human HBB ELISA Kit (AB235654)

Interpolated concentrations of native HBB in TF-1 Extract based on a 37.5 μg/mL extract load.

The concentrations of HBB were measured in duplicate and interpolated from the HBB standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HBB concentration was determined to be 4.5 ng/mL in TF-1 extract.

sELISA

Supplier Data

Sandwich ELISA - Human HBB ELISA Kit (AB235654)

Example of human HBB standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 34.1 pg/mL

Range

0.125 - 8 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human HBB ELISA Kit (ab235654) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of HBB protein in cell culture extracts, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human HBB with 34.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]

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Recovery

[ { "sample": "Serum", "range": null, "average": "= 102" }, { "sample": "EDTA Plasma", "range": null, "average": "= 107" }, { "sample": "Cell culture extracts", "range": null, "average": "= 90" }, { "sample": "Heparin Plasma", "range": null, "average": "= 101" }, { "sample": "Citrate plasma", "range": null, "average": "= 98" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hemoglobin also known as Hb or HBB is a protein found in red blood cells. It is a tetramer with a molecular mass of approximately 64.5 kDa composed of two alpha and two beta subunits. This protein is predominantly expressed in erythrocytes where it plays an important role in oxygen transport from the lungs to tissues and facilitates the return of carbon dioxide from tissues to the lungs for exhalation.

Biological function summary

Hemoglobin carries oxygen in blood and acts as a buffer in maintaining blood pH. Within the erythrocytes hemoglobin comprises one of the principal components of these cells each molecule binding up to four oxygen molecules. Hemoglobin exists as a complex with heme groups that directly interact with oxygen allowing hemoglobin to perform its respiratory function efficiently.

Pathways

Hemoglobin is important in the circulatory and respiratory pathways. It works in conjunction with proteins like myoglobin which assists with oxygen storage in muscles and cytochrome c which is important in the electron transport chain. Hemoglobin's function aligns with these pathways to maintain proper oxygen and energy levels throughout the body.

Hemoglobin abnormalities lead to conditions such as sickle cell anemia and thalassemia. Sickle cell anemia involves a mutation in the beta-globin gene resulting in deformed red blood cells that hinder oxygen delivery and cause pain due to blockages in blood vessels. Thalassemia results from reduced production of globin chains leading to anemia. These conditions involve aberrations in the hemoglobin or related proteins impacting overall health and requiring clinical management.

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Product protocols

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Target data

Involved in oxygen transport from the lung to the various peripheral tissues.. Hemopressin. Hemopressin acts as an antagonist peptide of the cannabinoid receptor CNR1 (PubMed : 18077343). Hemopressin-binding efficiently blocks cannabinoid receptor CNR1 and subsequent signaling (PubMed : 18077343).

See full target information HBA1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The journal of gene medicine 26:e3640 PubMed37989259

2023

Reducing the transcriptional read-through rate of a lentiviral vector for β-thalassemia gene therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahui Wu,Yuan Chen,Wenchen Shen,Jingzhi Zhang,Fanyi Zeng

Scientific reports 11:22121 PubMed34764389

2021

Arsenite exposure inhibits the erythroid differentiation of human hematopoietic progenitor CD34 cells and causes decreased levels of hemoglobin.

Applications

Unspecified application

Species

Unspecified reactive species

Guanghua Wan,Sebastian Medina,Haikun Zhang,Rong Pan,Xixi Zhou,Alicia M Bolt,Li Luo,Scott W Burchiel,Ke Jian Liu

Scientific reports 11:15646 PubMed34341431

2021

Salivary proteome of aphthous stomatitis reveals the participation of vitamin metabolism, nutrients, and bacteria.

Applications

Unspecified application

Species

Unspecified reactive species

Romina Hernández-Olivos,Mariagrazia Muñoz,Esteban Núñez,Paola Andrea Camargo-Ayala,Jenaro Garcia-Huidobro,Alfredo Pereira,Fabiane M Nachtigall,Leonardo S Santos,César Rivera

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human HBB ELISA Kit

Sandwich ELISA - Human HBB ELISA Kit (AB235654)

Sandwich ELISA - Human HBB ELISA Kit (AB235654)

Sandwich ELISA - Human HBB ELISA Kit (AB235654)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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Product promise