Human HBG2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human HBG2 with a sensitivity of 6.19 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 54.688 - 3500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 6.19 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Gamma chains make up the fetal hemoglobin F, in combination with alpha chains.
Alternative names
Hemoglobin subunit gamma-2, Gamma-2-globin, Hb F Ggamma, Hemoglobin gamma-2 chain, Hemoglobin gamma-G chain, HBG2
What's included?
Recommended products
Human HBG2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human HBG2 with a sensitivity of 6.19 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 54.688 - 3500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 6.19 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 4.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 13 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Tissue Extracts | Average % = 102 | Range 84 - 118 % |
Sample type Cell Lysate | Average % = 112 | Range 104 - 116 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human HBG2 ELISA kit (ab282314) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human HBG2 protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human HBG2 with 6.19 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human HBG2 is normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, human HBG2 facilitated gamma chain production continues into adulthood. Human HBG2 is also linked with blood coagulation, cellular oxidant detoxification, and hydrogen peroxide catabolic process.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Fetal hemoglobin also known as fetal Hb or HbF is a form of hemoglobin found in fetuses and neonates. HbF is composed of two alpha and two gamma globin chains and its molecular weight is around 64000 Daltons. This hemoglobin variant is mainly expressed in the red blood cells of the fetal liver and bone marrow. Expression of fetal hemoglobin typically declines after birth when beta globin chains replace gamma chains to form adult hemoglobin (HbA). However low levels of fetal hemoglobin can persist into adulthood in some individuals.
- Biological function summary
Fetal hemoglobin has a higher affinity for oxygen compared to adult hemoglobin. This characteristic allows efficient transfer of oxygen from maternal to fetal circulation which is vital for fetal development. Fetal hemoglobin is not part of any complex but its unique structure is adapted to its function in the fetal environment. The alpha and gamma globin chains together modulate its oxygen-binding properties ensuring proper oxygenation during fetal life.
- Pathways
The production and transition of fetal hemoglobin are linked to the globin gene switching pathway. This process involves the silencing of gamma globin genes and activation of beta globin genes postnatally a transition integral to producing adult hemoglobin. BCL11A and KLF1 are transcription factors that play essential roles in regulating this gene switching. Both proteins influence the repression of gamma globin genes therefore promoting the expression of beta globin.
- Associated diseases and disorders
Fetal hemoglobin is significant in conditions like sickle cell disease and beta thalassemia. In these disorders elevated levels of fetal hemoglobin can ameliorate symptoms by compensating for defective adult hemoglobin. Patients with higher fetal hemoglobin levels typically exhibit milder forms of these diseases. Therapeutic approaches aim to reactivate fetal hemoglobin expression through the modulation of proteins like BCL11A KLF1 and others involved in hemoglobin switching.
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2 product images
Sandwich ELISA - Human HBG2 ELISA Kit (ab282314)
Example of human HBG2 standard curve in 1X Cell Extraction Buffer PTR.
The HBG2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human HBG2 ELISA Kit (ab282314)
Interpolated concentrations of native HBG2 in human liver extract and K562 cell extract based on a 300 µg/mL extract load and 7.5 µg/mL extract load respectively.
The concentrations of HBG2 were measured in duplicate and interpolated from the HBG2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HBG2 concentration was determined to be 3.68 ng/mL in liver extract and 4.63 ng/mL in K562 cell extract.
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