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AB282314

Human HBG2 ELISA Kit

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Human HBG2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human HBG2 with a sensitivity of 6.19 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Hemoglobin subunit gamma-2, Gamma-2-globin, Hb F Ggamma, Hemoglobin gamma-2 chain, Hemoglobin gamma-G chain, HBG2

2 Images
Sandwich ELISA - Human HBG2 ELISA Kit (AB282314)
  • sELISA

Supplier Data

Sandwich ELISA - Human HBG2 ELISA Kit (AB282314)

Interpolated concentrations of native HBG2 in human liver extract and K562 cell extract based on a 300 µg/mL extract load and 7.5 µg/mL extract load respectively.

The concentrations of HBG2 were measured in duplicate and interpolated from the HBG2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HBG2 concentration was determined to be 3.68 ng/mL in liver extract and 4.63 ng/mL in K562 cell extract.

Sandwich ELISA - Human HBG2 ELISA Kit (AB282314)
  • sELISA

Supplier Data

Sandwich ELISA - Human HBG2 ELISA Kit (AB282314)

Example of human HBG2 standard curve in 1X Cell Extraction Buffer PTR.

The HBG2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6.19 pg/mL

Range

54.688 - 3500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

AB137096

Anti-fetal hemoglobin antibody [EPR9708(B)]

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Proteins & Peptides

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Primary Antibodies

AB209790

Anti-Ghrelin antibody [EPR20502]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ghrelin antibody [EPR20502] (AB209790)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human HBG2 ELISA kit (ab282314) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human HBG2 protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human HBG2 with 6.19 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Human HBG2 is normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, human HBG2 facilitated gamma chain production continues into adulthood. Human HBG2 is also linked with blood coagulation, cellular oxidant detoxification, and hydrogen peroxide catabolic process.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "13" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "84 - 118 %", "average": "= 102" }, { "sample": "Cell Lysate", "range": "104 - 116 %", "average": "= 112" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab282314-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB282314-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB282314-CMP15", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB282314-CMP14", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human HBG2 Lyophilized Recombinant Protein", "number":"AB282314-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB282314-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human HBG2 Capture Antibody", "number":"AB282314-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human HBG2 Detector Antibody", "number":"AB282314-CMP02", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB282314-CMP13", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB282314-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB282314-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB282314-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB282314-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fetal hemoglobin also known as fetal Hb or HbF is a form of hemoglobin found in fetuses and neonates. HbF is composed of two alpha and two gamma globin chains and its molecular weight is around 64000 Daltons. This hemoglobin variant is mainly expressed in the red blood cells of the fetal liver and bone marrow. Expression of fetal hemoglobin typically declines after birth when beta globin chains replace gamma chains to form adult hemoglobin (HbA). However low levels of fetal hemoglobin can persist into adulthood in some individuals.
Biological function summary

Fetal hemoglobin has a higher affinity for oxygen compared to adult hemoglobin. This characteristic allows efficient transfer of oxygen from maternal to fetal circulation which is vital for fetal development. Fetal hemoglobin is not part of any complex but its unique structure is adapted to its function in the fetal environment. The alpha and gamma globin chains together modulate its oxygen-binding properties ensuring proper oxygenation during fetal life.

Pathways

The production and transition of fetal hemoglobin are linked to the globin gene switching pathway. This process involves the silencing of gamma globin genes and activation of beta globin genes postnatally a transition integral to producing adult hemoglobin. BCL11A and KLF1 are transcription factors that play essential roles in regulating this gene switching. Both proteins influence the repression of gamma globin genes therefore promoting the expression of beta globin.

Fetal hemoglobin is significant in conditions like sickle cell disease and beta thalassemia. In these disorders elevated levels of fetal hemoglobin can ameliorate symptoms by compensating for defective adult hemoglobin. Patients with higher fetal hemoglobin levels typically exhibit milder forms of these diseases. Therapeutic approaches aim to reactivate fetal hemoglobin expression through the modulation of proteins like BCL11A KLF1 and others involved in hemoglobin switching.
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Product protocols

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Target data

Gamma chains make up the fetal hemoglobin F, in combination with alpha chains.
See full target information Hemoglobin subunit gamma-2
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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