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AB277402

Human HC-II ELISA Kit

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(1 Publication)

Human HC-II ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human HC-II in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

HCF2, SERPIND1, Heparin cofactor 2, Heparin cofactor II, Protease inhibitor leuserpin-2, Serpin D1, HC-II, HLS2

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ELISA - Human HC-II ELISA Kit (AB277402)
  • ELISA

Supplier Data

ELISA - Human HC-II ELISA Kit (AB277402)

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.23 ng/mL

Range

1.229 - 300 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human HC-II ELISA Kit (ab277402) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HC-II in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human HC-II coated on a 96-well plate. Standards and samples are pipetted into the wells and Human HC-II present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human HC-II antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human HC-II bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "89 - 149 %", "average": "= 118.5" }, { "sample": "Serum", "range": "83 - 145 %", "average": "= 116.4" }, { "sample": "Plasma", "range": "89 - 149 %", "average": "= 117.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277402-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277402-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277402-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277402-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human HC-II coated Microplate", "number":"AB277402-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277402-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human HC-II standard protein (Lyophilized)", "number":"AB277402-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human HC-II Antibody", "number":"AB277402-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"700X HRP-Streptavidin Concentrate", "number":"AB277402-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HC-II also known as heparin cofactor II is an enzyme that acts as a serine protease inhibitor. It mainly inhibits thrombin playing a significant role in the regulation of coagulation. HC-II has an approximate molecular mass of 65 kDa and is primarily expressed in the liver and circulates in plasma. The HC protein is similar to another anticoagulant protein antithrombin but is specifically activated by dermatan sulfate.
Biological function summary

Heparin cofactor II plays an essential role in maintaining hemostatic balance. It does not function as part of a larger complex but individually regulates thrombin activity. HC-II inhibits fibrin formation and platelet aggregation controlling thrombin’s role in the coagulation cascade. The activity of HC-II is important for keeping blood clot formation under control preventing excessive clot formation which can lead to vascular diseases.

Pathways

Heparin cofactor II works in the coagulation pathway where it closely interacts with thrombin. Dermatan sulfate enhances its inhibitory effects on thrombin distinct from the action of heparin on antithrombin. Its regulation of thrombin makes it an important component in the hemostatic pathway maintaining normal circulation by balancing blood clotting and bleeding.

Heparin cofactor II is associated with thrombotic disorders including venous thromboembolism. Deficiency or malfunctioning of HC-II can lead to an increased risk of thrombosis due to inadequate inhibition of thrombin. Besides HC-II is associated with atherosclerosis where its interaction with dermatan sulfate could influence the progression of vascular lesions. The protein's role in modulating thrombin activity further connects it to coagulation factor V which can also impact thrombotic disease processes.
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Product protocols

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Target data

Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.. Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils.. Isoform 2. Shows negligible inhibition, in vitro, of thrombin and tPA and no inhibition of factor Xa, in vitro.
See full target information SERPIND1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e21616 PubMed38027820

2023

Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyan Chen,Jingyang Li,Yilan You,Zhiwen Qian,Jiayu Liu,Ying Jiang,Ying Gu,Jianping Xiao,Yan Zhang
View all publications
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