JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB155433

Human HCC-1 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human HCC-1 ELISA Kit is a Sandwich ELISA for the measurement of Human HCC-1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

NCC2, SCYA14, CCL14, C-C motif chemokine 14, Chemokine CC-1/CC-3, HCC-1(1-74), NCC-2, Small-inducible cytokine A14, HCC-1/HCC-3

2 Images
Sandwich ELISA - Human HCC-1 ELISA Kit (AB155433)
  • sELISA

Unknown

Sandwich ELISA - Human HCC-1 ELISA Kit (AB155433)

Representative standard curve using ab155433

Sandwich ELISA - Human HCC-1 ELISA Kit (AB155433)
  • sELISA

Unknown

Sandwich ELISA - Human HCC-1 ELISA Kit (AB155433)

Representative standard curve using ab155433

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 7 pg/mL

Range

0.006 - 1.5 ng/mL

Assay Platform

Microplate

style
left-column

Complementary Products

Assay Kits

AB150686

Trichrome Stain Kit (Connective Tissue Stain)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Trichrome Stain Kit (Connective Tissue Stain) (AB150686)

  • 5
  • 5
Primary Antibodies

AB192877

Anti-MIP-3 beta/CCL19 antibody [EPR7044(2)]

RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-MIP-3 beta/CCL19 antibody [EPR7044(2)] (AB192877)

  • 0
  • 0
Primary Antibodies

AB16502

Anti-NF-kB p65 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 antibody (AB16502)

  • 4
  • 40
Primary Antibodies

AB224188

Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Macrophage Inflammatory Protein 3 alpha antibody [EPR22376-58] (AB224188)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's HCC-1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human HCC-1 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human HCC-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and HCC-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HCC-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "102 - 114 %", "average": "= 108.4" }, { "sample": "Plasma", "range": "90 - 108 %", "average": "= 102.1" }, { "sample": "Cell culture media", "range": "105 - 125 %", "average": "= 110" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155433-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155433-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155433-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"HCC-1 Microplate (12 x 8 wells)", "number":"AB155433-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB155433-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155433-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB155433-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155433-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human HCC-1", "number":"AB155433-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human HCC-1 Standard (lyophilized)", "number":"AB155433-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HCC-1 also known as CCL14a is a chemokine belonging to the C-C motif chemokine family. This protein has a molecular mass of approximately 10 kDa. HCC-1 is mainly expressed in the liver but can also be found in other tissues like the spleen lung and bone marrow. It plays a direct role in chemotaxis guiding the movement of immune cells to specific sites within the body mainly influencing monocyte activities.
Biological function summary

HCC-1 affects immune response by interacting with chemokine receptors. It binds particularly to CCR1 and CCR5 receptors on the cell surface. Though HCC-1 is not usually part of a larger protein complex it functions closely with other chemokines in modulating leukocyte behavior. These interactions highlight its role in immune surveillance and inflammatory response demonstrating its significance in orchestrating an effective immune defense.

Pathways

HCC-1 is actively involved in chemokine signaling pathways and has a role in inflammation-related processes. Its interaction with CCR1 and CCR5 connects it directly to the MAPK signaling pathway impacting cell proliferation and survival. HCC-1 similar to proteins such as MCP-1 and RANTES plays a part in guiding immune cells during inflammation therefore contributing to the overall coordination of the immune response.

HCC-1 shows a connection with inflammatory diseases and certain types of cancer. Elevated levels of this chemokine associate with rheumatoid arthritis where it may influence disease progression by attracting inflammatory cells to the joints. Additionally HCC-1 has links to liver cancer processes particularly hepatocellular carcinoma due to its high expression in liver tissue. In these contexts proteins like CCR5 are also involved mediating critical pathways that exacerbate disease conditions.
style
left-column

Product protocols

style
left-column

Target data

Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induces intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and is inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes, eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.
See full target information CCL14
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com