Human HCC-1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human HCC-1 in Human in Plasma, Cell culture supernatant, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.006 - 1.5 ng/mL
- Sensitivity
- < 7 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induces intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and is inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes, eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.
Alternative names
NCC2, SCYA14, CCL14, C-C motif chemokine 14, Chemokine CC-1/CC-3, HCC-1(1-74), NCC-2, Small-inducible cytokine A14, HCC-1/HCC-3
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Human HCC-1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human HCC-1 in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.006 - 1.5 ng/mL
- Assay Platform
- Microplate
- Sensitivity
- < 7 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 108.4 | Range 102 - 114 % |
Sample type Plasma | Average % = 102.1 | Range 90 - 108 % |
Sample type Cell culture media | Average % = 110 | Range 105 - 125 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's HCC-1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human HCC-1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human HCC-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and HCC-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HCC-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HCC-1 also known as CCL14a is a chemokine belonging to the C-C motif chemokine family. This protein has a molecular mass of approximately 10 kDa. HCC-1 is mainly expressed in the liver but can also be found in other tissues like the spleen lung and bone marrow. It plays a direct role in chemotaxis guiding the movement of immune cells to specific sites within the body mainly influencing monocyte activities.
- Biological function summary
HCC-1 affects immune response by interacting with chemokine receptors. It binds particularly to CCR1 and CCR5 receptors on the cell surface. Though HCC-1 is not usually part of a larger protein complex it functions closely with other chemokines in modulating leukocyte behavior. These interactions highlight its role in immune surveillance and inflammatory response demonstrating its significance in orchestrating an effective immune defense.
- Pathways
HCC-1 is actively involved in chemokine signaling pathways and has a role in inflammation-related processes. Its interaction with CCR1 and CCR5 connects it directly to the MAPK signaling pathway impacting cell proliferation and survival. HCC-1 similar to proteins such as MCP-1 and RANTES plays a part in guiding immune cells during inflammation therefore contributing to the overall coordination of the immune response.
- Associated diseases and disorders
HCC-1 shows a connection with inflammatory diseases and certain types of cancer. Elevated levels of this chemokine associate with rheumatoid arthritis where it may influence disease progression by attracting inflammatory cells to the joints. Additionally HCC-1 has links to liver cancer processes particularly hepatocellular carcinoma due to its high expression in liver tissue. In these contexts proteins like CCR5 are also involved mediating critical pathways that exacerbate disease conditions.
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2 product images
Sandwich ELISA - Human HCC-1 ELISA Kit (ab155433)
Representative standard curve using ab155433
Sandwich ELISA - Human HCC-1 ELISA Kit (ab155433)
Representative standard curve using ab155433
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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