Human hCG ELISA Kit

Product details

Human hCG ELISA Kit ab100533 is a sandwich ELISA to measure Human hCG in serum, plasma, cell culture supernatant with a sensitivity of 50 pg/ml.

How the assay works

This assay employs an antibody specific for Human hCG coated on a 96-well plate. Standards and samples are pipetted into the wells and hCG present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human hCG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of hCG bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human hCG ELISA Kit ab100533 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Add prepared biotin antibody to each well. Incubate at room temperature.
3. Add prepared Streptavidin solution. Incubate at room temperature
4. Add TMB One-Step Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.

Optimization may be required with urine samples