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AB300311

Human HDAC4 ELISA Kit

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Human HDAC4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human HDAC4 with a sensitivity of 94.776 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

KIAA0288, HDAC4, Histone deacetylase 4, HD4

2 Images
Sandwich ELISA - Human HDAC4 ELISA Kit (AB300311)
  • sELISA

Supplier Data

Sandwich ELISA - Human HDAC4 ELISA Kit (AB300311)

Interpolated concentrations of human HDAC4 in Jurkat cell extract.

Interpolated concentration of native HDAC4 was measured in duplicate at different sample concentrations. Undiluted samples are 500 μg/mL Jurkat cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human HDAC4 ELISA Kit (AB300311)
  • sELISA

Supplier Data

Sandwich ELISA - Human HDAC4 ELISA Kit (AB300311)

Example of human HDAC4 standard curve in 1X Cell Extraction Buffer PTR.

Example of human HDAC4 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 94.776 pg/mL

Range

312.5 - 20000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "83 - 90 %", "average": "= 85" } ]
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What's included?

{ "values": { "96Tests": { "sellingSize": "96 Tests", "publicAssetCode":"ab300311-96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB300311-CMP20", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB300311-CMP17", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB300311-CMP19", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB300311-CMP23", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB300311-CMP18", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human HDAC4 Lyophilized Recombinant Protein", "number":"AB300311-CMP14", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human HDAC4 Capture Antibody", "number":"AB300311-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB300311-CMP22", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB300311-CMP21", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB300311-CMP16", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human HDAC4 Detector Antibody", "number":"AB300311-CMP13", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HDAC4 also known as Histone Deacetylase 4 functions in the regulation of gene expression through chromatin remodeling. This protein removes acetyl groups from histone proteins causing chromatin compaction and transcriptional repression. HDAC4 has a molecular weight of approximately 140 kDa. The protein is found in various tissues including brain heart and skeletal muscle and plays essential roles in various cellular processes. Alternate names for HDAC4 include KIAA0288 and HD4.
Biological function summary

The role of HDAC4 extends beyond chromatin remodeling as it serves as a critical regulator in several cellular functions. As part of a complex HDAC4 interacts with other proteins to control cell cycle differentiation and apoptosis. It cooperates with transcriptional repressors like MEF2 and RUNX2 influencing various signaling pathways. The protein's activity impacts neuronal survival muscle development and cardiac hypertrophy through its regulatory mechanisms.

Pathways

HDAC4 integrates into significant signaling networks notably the MAPK and calcium-calmodulin signaling pathways. Within the MAPK pathway HDAC4 associates with proteins like MEF2 impacting cellular growth and differentiation processes. In the calcium-calmodulin pathway HDAC4 affects gene transcription via its interaction with the phosphatase calcineurin linking intracellular calcium levels with transcriptional responses.

HDAC4 has associations with neurological disorders and cancer. Mutations and dysregulation in HDAC4 are linked to neuronal disorders such as Huntington's disease. In cancer HDAC4 interacts with other oncogenic proteins such as p53 influencing tumor progression and resistance to therapy. Consequently HDAC4 presents as a potential target for therapeutic intervention in these disease contexts.
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Product protocols

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Target data

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1 (PubMed : 27708256).
See full target information HDAC4
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websiteProtocolBooklet
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Product promise

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