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AB284005

Human HDHD2 ELISA Kit

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Human HDHD2 ELISA Kit is a Sandwich ELISA for the measurement of Human HDHD2 in Human in Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

Haloacid dehalogenase-like hydrolase domain-containing protein 2, HDHD2

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Sandwich ELISA - Human HDHD2 ELISA Kit (AB284005)
  • sELISA

Supplier Data

Sandwich ELISA - Human HDHD2 ELISA Kit (AB284005)

Standard curve analysis of ab284005. For reference use only.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 21 pg/mL

Range

0.031 - 2 ng/mL

Assay time

4h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Proteins & Peptides

AB101940

Recombinant Human HDHD2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human HDHD2 protein (His tag N-Terminus) (AB101940)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human HDHD2 ELISA Kit (ab284005) is designed for detection of HDHD2 in human CSF and cell lysate samples.

This assay employs a quantitative sandwich enzyme immunoassay technique that measures human HDHD2 in approximately 4 hours. A polyclonal antibody specific for human HDHD2 has been pre-coated onto a 96-well microplate with removable strips. HDHD2 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for human HDHD2, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.8" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab284005-1x96Tests", "assetComponentDetails": [ { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB284005-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB284005-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human HDHD2 Antibody", "number":"AB284005-CMP03", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB284005-CMP10", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human HDHD2 Standard", "number":"AB284005-CMP05", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB284005-CMP02", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB284005-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Diluent M Concentrate", "number":"AB284005-CMP01", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB284005-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human HDHD2 Microplate", "number":"AB284005-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HDHD2 also known as haloacid dehalogenase-like hydrolase domain-containing protein 2 is an enzyme with a molecular weight of approximately 22 kDa. It primarily functions as a phosphatase which removes phosphate groups from specific substrates. HDHD2 is expressed in several tissues including the liver and brain. Its activity is integral to regulating phosphate metabolism and maintaining cellular homeostasis.
Biological function summary

HDHD2 hydrolyzes phosphoserines and plays an essential role in dephosphorylation processes. It is a component of protein complexes that control phosphorylation states within cells directly affecting protein functions. Through these actions HDHD2 influences cellular signaling and metabolism contributing to physiological processes such as enzyme regulation and substrate modification.

Pathways

HDHD2 participates in signaling pathways related to phosphate metabolism and energy balance. It is related to the energy production pathway and the regulation of cellular signaling cascades. This protein interacts with other enzymes like PPARG and PTPMT1 coordinating the maintenance of phosphate balance across cellular compartments.

Abnormalities in HDHD2 function can be linked to metabolic disorders including diabetes and obesity. Its disturbance affects phosphate homeostasis and potentially contributes to insulin resistance. HDHD2 interacts with proteins such as insulin receptors and SREBP-1c implicating it in metabolic dysregulation associated with these conditions.
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Product protocols

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Target data

See full target information Haloacid dehalogenase-like hydrolase domain-containing protein 2
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websiteProtocolBooklet
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Product promise

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