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AB125961

Human HDL ELISA Kit

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(4 Publications)

Human HDL ELISA Kit is a Competitive ELISA for the measurement of Human HDL in Human in Biofluids samples.
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Competitive ELISA - Human HDL ELISA Kit (AB125961)
  • cELISA

Supplier Data

Competitive ELISA - Human HDL ELISA Kit (AB125961)

Representative Standard Curve using ab125961

Key facts

Detection method

Colorimetric

Sample types

Plasma, Milk, Serum

Reacts with

Human

Assay type

Competitive

Sensitivity

= 1 µg/mL

Range

6.25 - 50 µg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's High-Density Lipoprotein (HDL) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human HDL in plasma, serum and milk samples.

A HDL specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated HDL is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of HDL captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab125961-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Biotinylated Human HDL (Lyophilized)", "number":"AB125961-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB125961-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"HDL Standard", "number":"AB125961-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"HDL Microplate (12 x 8 well strips)", "number":"AB125961-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent M Concentrate", "number":"AB125961-CMP02", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB125961-CMP09", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB125961-CMP08", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB125961-CMP07", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB125961-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HDL also known as high-density lipoprotein serves an important role in lipid transport. It acts mainly in reverse cholesterol transport a process where HDL picks up cholesterol from peripheral tissues and delivers it back to the liver for excretion. The mass of HDL particles can vary but typically range between 8 to 12 nm in diameter depending on their lipid and protein content. HDL is primarily expressed in the liver and intestines. The principal protein components of HDL include apolipoprotein A-I (apoA-I) and apolipoprotein A-II (apoA-II) which are essential for HDL's structure and function.
Biological function summary

Our high-density lipoprotein participates in cholesterol efflux a critical step in cholesterol homeostasis preventing cellular cholesterol accumulation. HDL acts as part of a complex with several enzymes and transporter proteins such as lecithin-cholesterol acyltransferase (LCAT) and ATP-binding cassette transporters A1 and G1 (ABCA1 ABCG1). These interactions enhance the lipid transport capability enabling HDL to remodel and carry varied cargo including cholesterol and antioxidants. HDL's role in antioxidation and inflammation modulation has been studied extensively highlighting its importance beyond lipid transport.

Pathways

The involvement of high-density lipoprotein is important in the larger cholesterol metabolism and cardiovascular protection pathways. HDL participates in the reverse cholesterol transport pathway linked closely with lipid regulatory proteins like LCAT and cholesterol ester transfer protein (CETP). Furthermore HDL interacts with endothelial nitric oxide synthase (eNOS) emphasizing its role in cardiovascular health by maintaining endothelial function. This connection highlights its contribution to reducing the risk of atherosclerosis a pathway critical for vascular health.

Links between high-density lipoprotein and cardiovascular diseases like atherosclerosis and coronary artery disease are significant. Low levels of HDL are often associated with increased risk of these conditions. Beyond lipoprotein components proteins like CETP and scavenger receptor BI (SR-BI) have roles in these diseases affecting HDL metabolism and function. Research continues to explore the therapeutic potential of targeting HDL metabolism to mitigate these cardiovascular risks considering its intricate relationship with these proteins and pathways.
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Product protocols

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Target data

See full target information HDL
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Folia morphologica : PubMed39387397

2024

Cardioprotective effect of Perakine against myocardial ischemia-reperfusion injury of type-2 diabetic rat in Langendorff-Perfused Rat Hearts: Role of TLR4/NF-kB signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jinli Wang,Li Fan

Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis 27:10760296211035446 PubMed34702084

2021

miR-124 Is Downregulated in Serum of Acute Cerebral Infarct Patients and Shows Diagnostic and Prognostic Value.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Zhou,Lizhong Qi

Diabetes 68:2287-2300 PubMed31506344

2019

Diabetic Nephropathy Alters the Distribution of Circulating Angiogenic MicroRNAs Among Extracellular Vesicles, HDL, and Ago-2.

Applications

Unspecified application

Species

Unspecified reactive species

Barend W Florijn,Jacques M G J Duijs,Johannes H Levels,Geesje M Dallinga-Thie,Yanan Wang,Anita N Boing,Yuana Yuana,Wendy Stam,Ronald W A L Limpens,Yu Wah Au,Rienk Nieuwland,Ton J Rabelink,Marlies E J Reinders,Anton Jan van Zonneveld,Roel Bijkerk

International journal of molecular sciences 18: PubMed28230785

2017

The Application of a Modified d-ROMs Test for Measurement of Oxidative Stress and Oxidized High-Density Lipoprotein.

Applications

Unspecified application

Species

Unspecified reactive species

Fumiaki Ito,Tomoyuki Ito,Chinatsu Suzuki,Tomoyo Yahata,Kazuyuki Ikeda,Kenji Hamaoka
View all publications
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