Products
Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesOur latest ELISA kit: Human Tau (phospho T217) - Intracellular
Highly sensitive kit offering the most promising biomarkers for Alzheimer’s disease diagnostics. Learn about all product ranges with our product overviews.
Resources
Featured events
Make new connections at our global events.
Support
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Human HDL ELISA Kit is a Competitive ELISA kit for the measurement of Human HDL in Human in Plasma, Milk, Serum samples.
Alternative names=High Density Lipoprotein
- Detection method
Colorimetric
- Sample types
Plasma, Milk, Serum
- Assay type
Competitive
- Reactive species
Human
- Range
6.25 - 50 µg/mL
- Assay time
3h
- Sensitivity
= 1 µg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
Images
What's included?
Recommended products
Human HDL ELISA Kit is a Competitive ELISA kit for the measurement of Human HDL in Human in Plasma, Milk, Serum samples.
Alternative names=High Density Lipoprotein
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Milk, Serum
- Assay type
Competitive
- Reactive species
Human
- Range
6.25 - 50 µg/mL
- Assay time
3h
- Assay Platform
Microplate
- Sensitivity
= 1 µg/mL
- General recovery
96%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 5.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 10.3 |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
Multi
- Storage information
Please refer to protocols
Notes
Abcam's High-Density Lipoprotein (HDL) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human HDL in plasma, serum, milk, and cell culture supernatant.
A HDL specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated HDL is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of HDL captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com