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AB240688

Human HE4 ELISA Kit

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Human HE4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human HE4 with a sensitivity of 5.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

HE4, WAP5, WFDC2, WAP four-disulfide core domain protein 2, Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5

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ELISA - Human HE4 ELISA Kit (AB240688)
  • ELISA

Supplier Data

ELISA - Human HE4 ELISA Kit (AB240688)
Sandwich ELISA - Human HE4 ELISA Kit (AB240688)
  • sELISA

Unknown

Sandwich ELISA - Human HE4 ELISA Kit (AB240688)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HE4 concentration was determined to be 7067 pg/mL with a range of 4099 - 12,026 pg/mL.

Sandwich ELISA - Human HE4 ELISA Kit (AB240688)
  • sELISA

Unknown

Sandwich ELISA - Human HE4 ELISA Kit (AB240688)

Example of human HE4 standard curve in Sample Diluent NS + Enhancer.

The HE4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human HE4 ELISA Kit (AB240688)
  • sELISA

Unknown

Sandwich ELISA - Human HE4 ELISA Kit (AB240688)

Example of human HE4 standard curve in Sample Diluent 25BS + Enhancer. The HE4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human HE4 ELISA Kit (AB240688)
  • sELISA

Unknown

Sandwich ELISA - Human HE4 ELISA Kit (AB240688)

Interpolated concentrations of native HE4 in human serum, plasma (citrate), and plasma (EDTA) samples. The concentrations of HE4 were measured in duplicates, interpolated from the HE4 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HE4 concentration was determined to be 5,723 pg/mL in serum, 5,017 pg/mL in plasma (citrate), and 5,396 pg/mL in plasma (EDTA).

Sandwich ELISA - Human HE4 ELISA Kit (AB240688)
  • sELISA

Unknown

Sandwich ELISA - Human HE4 ELISA Kit (AB240688)

Interpolated concentrations of native HE4 in human saliva, urine, and cell culture supernatant samples. The concentrations of HE4 were measured in duplicates, interpolated from the HE4 standard curves and corrected for sample dilution. Undiluted samples are as follows : saliva 1 : 1,000, urine 1 : 1,000, and OVCAR-3 cell culture supernatant 1 : 1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HE4 concentration was determined to be 1,405 ng/mL in saliva, 1,237 ng/mL in urine, and 530 ng/mL in OVCAR-3 cell culture supernatant.

Sandwich ELISA - Human HE4 ELISA Kit (AB240688)
  • sELISA

Unknown

Sandwich ELISA - Human HE4 ELISA Kit (AB240688)

Human HE4 Standard Curve Comparison. Standard Curve comparison between human HE4 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.2 pg/mL

Range

23.4 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human HE4 ELISA Kit ab240688 is a rapid single-wash 90-min Sandwich ELISA to measure Human HE4 in cell culture supernatant, citrate plasma, EDTA plasma, saliva, serum, urine. This SimpleStep sensitivity is 5.2 pg/mL.

How the assay works

Human HE4 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human HE4 ELISA Kit ab240688 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-HE4 antibody [EPR16658-72] - BSA and Azide free (Capture) ab245100
- Anti-HE4 antibody [EPR16658-65] - BSA and Azide free (Detector) ab245100
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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "98 - 112 %", "average": "= 106" }, { "sample": "Serum", "range": "99 - 116 %", "average": "= 108" }, { "sample": "EDTA Plasma", "range": "111 - 119 %", "average": "= 115" }, { "sample": "Saliva", "range": "111 - 123 %", "average": "= 118" }, { "sample": "Urine", "range": "106 - 111 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "88 - 115 %", "average": "= 100" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Human Epididymis Protein 4 (HE4) also known as WAP four-disulfide core domain protein 2 (WFDC2) is a small glycoprotein of approximately 13 kDa. HE4 is most highly expressed in the epithelial cells of reproductive tissues like the epididymis but also in tissues such as the respiratory and urogenital tracts. HE4 is secreted into the extracellular space where it functions and is found in normal range in different body fluids. This secreted protein has gained attention due to its use in various diagnostic tests such as the HE4 test and HE4 assay utilizing HE4 antibodies.
Biological function summary

HE4 participates in innate immune response and epithelial cell protection. HE4 protein plays a role in the modulation of antiprotease activity as it is involved in inhibiting serine proteases. It is not part of a larger protein complex but interacts with multiple protease signaling pathways. HE4 is often studied for its role in modulating protein activity at epithelial barriers contributing to tissue homeostasis and protective mechanisms against infections.

Pathways

HE4 interacts within signaling cascades such as the innate immune pathways and extracellular matrix organization. It links to proteins within these pathways like serine proteases and other WFDC family proteins. HE4 influences protease activity regulation an essential component of tissue remodeling and inflammatory response impacting overall pathway dynamics.

HE4 has strong associations with ovarian cancer and endometrial cancer. Its overexpression in these malignancies makes it a valuable biomarker for disease diagnosis and prognosis. HE4 interacts with cancer-related proteins such as CA125 enhancing its utility in combinatory diagnostic approaches. The HE4 test and HE4 ELISA kit price underline its significance in clinical settings for monitoring these cancers where timely detection influences treatment outcomes.
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Product protocols

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Target data

Broad range protease inhibitor.
See full target information WFDC2
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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