AB108862

Human Heat Shock Protein 27 ELISA Kit (HSP27)

Name: Human Heat Shock Protein 27 ELISA Kit (HSP27) (ab108862) | Abcam
Brand: Abcam Limited
SKU: AB108862

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(5 Publications)

Human Heat Shock Protein 27 ELISA Kit (HSP27) is a Sandwich ELISA for the measurement of Human Heat Shock Protein 27 (HSP27) in Human in Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27

1 Images

Sandwich ELISA - Human Heat Shock Protein 27 ELISA Kit (HSP27) (AB108862)

sELISA

Supplier Data

Sandwich ELISA - Human Heat Shock Protein 27 ELISA Kit (HSP27) (AB108862)

Representative Standard Curve using ab108862.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue, Milk, Serum, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

> 0.16 ng/mL

Range

1.25 - 20 ng/mL

Assay time

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's Heat Shock Protein 27 (HSP27) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HSP27 levels in cell culture samples, milk, serum, plasma and tissue extract.

A HSP27 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a HSP27 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of HSP27 captured in plate.

Get results in 90 minutes with Human Heat Shock Protein 27 ELISA Kit (HSP27) (ab193757) from our SimpleStep ELISA^® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heat Shock Protein 27 (HSP27) also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kDa. It is expressed in various tissues including muscle heart and brain and responds to stress stimuli such as heat shock oxidative stress and inflammation. HSP27 functions as a molecular chaperone helping to stabilize unfolded proteins and prevent aggregation. Its expression increases significantly in cells undergoing stress playing a protective role in maintaining protein homeostasis.

Biological function summary

The small HSP protein functions in cellular processes like apoptosis cytoskeletal organization and protein folding. It does not act alone but often interacts with other proteins to form complexes that perform specific functions efficiently. HSP27 can bind to actin filaments influencing cell shape and motility. It also plays a role in preventing cell death by inhibiting apoptosome formation and modulating caspase activation. The dual role of protecting cells and modulating cell growth makes HSP27 an important tool for cells under stress.

Pathways

HSP27 interacts with the mitogen-activated protein kinase (MAPK) and PI3K/AKT signaling pathways. These pathways regulate cell survival growth and differentiation. Within these pathways HSP27 is linked to proteins such as Akt and MAPK which are critical for relaying survival and growth signals. By interacting with these molecules HSP27 helps to mediate the cell's response to external stressors and promotes cell survival under adverse conditions.

HSP27 is significant in cancer and neurodegenerative diseases. Its overexpression often occurs in various cancers including breast and prostate cancer where it contributes to tumor growth and resistance to chemotherapy. In neurodegenerative diseases like Alzheimer's disease altered expression and functioning of HSP27 can lead to aggregation of proteins such as tau contributing to disease pathology. The link between HSP27 and these proteins shows its potential as a therapeutic target for modulating disease progression.

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Product protocols

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Target data

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed : 10383393, PubMed : 20178975). Plays a role in stress resistance and actin organization (PubMed : 19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed : 23728742).

See full target information HSPB1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular neuroscience 16:878673 PubMed35573833

2022

TAT-HSP27 Peptide Improves Neurologic Deficits Reducing Apoptosis After Experimental Subarachnoid Hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Yan Zhou,Jing-Yi Sun,Wei-Qi Wang,Shu-Xian Li,Han-Xia Li,Hui-Juan Yang,Ming-Feng Yang,Hui Yuan,Zong-Yong Zhang,Bao-Liang Sun,Jin-Xiang Han

SpringerPlus 3:635 PubMed25392804

2014

Plasma heat shock protein 27 is associated with coronary artery disease, abdominal aortic aneurysm and peripheral artery disease.

Applications

Unspecified application

Species

Unspecified reactive species

Cao Jin,Victoria L Phillips,Michael Ja Williams,Andre M van Rij,Gregory T Jones

Oncotarget 5:506-18 PubMed24504108

2014

Quantitative proteomic analysis reveals potential diagnostic markers and pathways involved in pathogenesis of renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole M A White,Olena Masui,Leroi V Desouza,Olga Krakovska,Shereen Metias,Alexander D Romaschin,R John Honey,Robert Stewart,Kenneth Pace,Jason Lee,Michael As Jewett,Georg A Bjarnason,K W Michael Siu,George M Yousef

American journal of physiology. Lung cellular and molecular physiology 306:L207-15 PubMed24242011

2013

Newborn rat response to single vs. combined cGMP-dependent pulmonary vasodilators.

Applications

Species

Unspecified reactive species

Masahiro Enomoto,Amish Jain,Jingyi Pan,Yulia Shifrin,Todd Van Vliet,Patrick J McNamara,Robert P Jankov,Jaques Belik

SpringerPlus 2:571 PubMed24255864

2013

Cortical non-aneurysmal subarachnoid hemorrhage post-carotid endarterectomy: a case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Gopiga Thanabalasundaram,Silvia Hernández-Durán,Thabele Leslie-Mazwi,Christopher S Ogilvy

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Heat Shock Protein 27 ELISA Kit (HSP27)

Sandwich ELISA - Human Heat Shock Protein 27 ELISA Kit (HSP27) (AB108862)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Primary Antibodies

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise