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AB108861

Human Heat Shock Protein 47 ELISA Kit (SERPINH1)

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(2 Publications)

Human Heat Shock Protein 47 ELISA Kit (SERPINH1) is a Sandwich (quantitative) ELISA for the measurement of Human Heat Shock Protein 47 (SERPINH1) in Human in Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

CBP1, CBP2, HSP47, SERPINH2, PIG14, SERPINH1, Serpin H1, 47 kDa heat shock protein, Arsenic-transactivated protein 3, Cell proliferation-inducing gene 14 protein, Collagen-binding protein, Rheumatoid arthritis-related antigen RA-A47, AsTP3, Colligin

1 Images
Sandwich ELISA - Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (AB108861)
  • sELISA

Supplier Data

Sandwich ELISA - Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (AB108861)

Representative Standard Curve using ab108861.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Plasma, Serum, Tissue, Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.54 ng/mL

Range

1.563 - 100 ng/mL

Assay time

5h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Heat Shock Protein 47 (SERPINH1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HSP47 levels in Human plasma, milk, serum, cell lysate, cerebrospinal fluid and tissue samples.

A HSP47 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a HSP47 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of HSP47 captured in plate.

Get results in 90 minutes with Human Heat Shock Protein 47 ELISA Kit (SERPINH1) (ab202407) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.6" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108861-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108861-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"HSP47 Standard", "number":"AB108861-CMP04", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human HSP47 Antibody", "number":"AB108861-CMP01", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108861-CMP05", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB108861-CMP10", "productcode":"AB108861" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108861-CMP07", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108861-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"HSP47 Microplate (12 x 8 well strips)", "number":"AB108861-CMP03", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108861-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108861-CMP11", "productcode":"AB108861" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp47 also known as the heat shock protein 47 is a molecular chaperone with a molecular weight of approximately 47 kDa. It mainly resides in the endoplasmic reticulum and participates in collagen synthesis. Hsp47 binds specifically to unfolded procollagen chains ensures their proper folding and prevents premature aggregation. Due to its specialized function Hsp47 is highly expressed in collagen-producing cells like fibroblasts. Researchers commonly study this protein using techniques like Hsp47 ELISA to measure its expression or activity levels.
Biological function summary

Hsp47 ensures the quality control of collagen within the secretory pathway. It does not form part of any large protein complexes but operates closely with collagen molecules. By stabilizing procollagen's triple-helix structure Hsp47 plays an important role in collagen biosynthesis. Dysfunctional collagen processes can arise without this protein's presence potentially leading to cellular stress or structural weaknesses within tissues rich in collagen.

Pathways

Hsp47's activity integrates into the collagen synthesis and secretion pathways. This protein is related to other molecular chaperones such as Hsp70 that assist in maintaining protein homeostasis within the cell. Hsp47’s function links to fibrosis pathways because of its role in collagen maturation. In terms of fibrotic pathologies this involvement makes the chaperoning of procollagen a critical control point.

Disrupted Hsp47 function frequently associates with fibrotic conditions and osteogenesis imperfecta. Fibrosis results from excessive collagen deposition in which Hsp47 is deeply implicated. Additionally certain cancer types have shown altered Hsp47 expression correlating with increased cancer cell invasiveness due to extracellular matrix remodeling. Studies have noted interactions between Hsp47 and TGF-beta signaling in fibrotic tissues reinforcing its role in pathological collagen turnover.
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Product protocols

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Target data

Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
See full target information SERPINH1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Proteomics 16:2718-2731 PubMed27459910

2016

Skin aging caused by intrinsic or extrinsic processes characterized with functional proteomics.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-You Fang,Pei-Wen Wang,Chun-Hsun Huang,Mu-Hong Chen,Yun-Ru Wu,Tai-Long Pan

Protein & cell 6:589-598 PubMed26091621

2015

Involvement of collagen-binding heat shock protein 47 in scleroderma-associated fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Chu,Ting Wu,Wenyu Wu,Wenzhen Tu,Shuai Jiang,Sidi Chen,Yanyun Ma,Qingmei Liu,Xiaodong Zhou,Li Jin,Jiucun Wang
View all publications
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