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AB108736

Human Anti-Helicobacter pylori IgG ELISA Kit

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(6 Publications)

Human Anti-Helicobacter pylori IgG ELISA Kit is an indirect ELISA for the qualitative detection of IgG class antibodies against Helicobacter pylori in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included
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Indirect ELISA - Human Anti-Helicobacter pylori IgG ELISA Kit (AB108736)
  • I-ELISA

Supplier Data

Indirect ELISA - Human Anti-Helicobacter pylori IgG ELISA Kit (AB108736)

Representative Standard Curve using ab108736

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Sensitivity

= 3 U/mL

Range

3 - 150 U/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Helicobacter pylori IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Helicobacter pylori in Human serum and plasma.

A 96-well plate has been precoated with Helicobacter pylori antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Helicobacter pylori-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Helicobacter pylori IgG sample captured in plate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 13, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 13, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 19, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 19, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108736-1x96Tests", "assetComponentDetails": [ { "size":"1 x 2 mL", "name":"Helicobacter pylori IgG Standard D – 150 U/mL", "number":"AB108736-CMP07", "productcode":"" }, { "size":"1 x 2 mL", "name":"Helicobacter pylori IgG Standard A – 0 U/mL", "number":"AB108736-CMP04", "productcode":"" }, { "size":"1 x 2 mL", "name":"Helicobacter pylori IgG Standard B – 15 U/mL", "number":"AB108736-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Helicobacter pylori (IgG) Coated Microplate (12 x 8 wells)", "number":"AB108736-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108736-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"Helicobacter pylori anti-IgG HRP conjugate", "number":"AB108736-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108736-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108736-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108736-CMP12", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgG Sample Diluent", "number":"AB108736-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108736-CMP11", "productcode":"" }, { "size":"1 x 2 mL", "name":"Helicobacter pylori IgG Standard C – 75 U/mL", "number":"AB108736-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Helicobacter pylori IgG often referred to as H. pylori IgG is an immunoglobulin produced in response to infection by the bacterium Helicobacter pylori. This bacterium is known for its spiral shape and Gram-negative characteristics. The molecular weight of the IgG antibody varies but generally is around 150 kDa. It is primarily expressed in response to colonization in the gastric mucosa which leads to increased immune response detectable in the bloodstream. The detection of H. pylori IgG in serological assays helps in diagnosing active or previous H. pylori infections.
Biological function summary

Antibodies such as IgG are part of the immune response working to neutralize pathogens like Helicobacter pylori. H. pylori IgG is not part of a larger protein complex but it interacts directly with antigens on the surface of the bacterial cells. By binding to these antigens H. pylori IgG plays a critical role in opsonization which facilitates the recognition and destruction of the bacteria by immune cells thereby contributing to host defense mechanisms.

Pathways

The H. pylori IgG operates within the broader context of the adaptive immune response pathway. This pathway involves numerous other proteins such as immunoglobulin M (IgM) and T-cell receptors. Together these components coordinate to mount an effective immune response against H. pylori as well as other pathogens. The H. pylori infection triggers a cascade in the immune system that leads to the activation of B cells which produce specific IgG antibodies tailored to recognize the pathogen.

Helicobacter pylori infection links closely to chronic gastritis and peptic ulcers. The presence of H. pylori IgG helps clinicians to evaluate such conditions related to gastric mucosal inflammation. These antibodies facilitate the detection of persistent infection contributing to the diagnosis and subsequent treatment strategies. The protein cytokine interleukin-8 (IL-8) also plays an essential role in inflammatory responses during H. pylori infection demonstrating the interconnectedness of immune components in addressing gastric diseases.
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Product protocols

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Target data

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Annals of medicine 54:379-392 PubMed35114859

2022

Synergism interaction between genetic polymorphisms in drug metabolizing enzymes and NSAIDs on upper gastrointestinal haemorrhage: a multicenter case-control study.

Applications

Unspecified application

Species

Unspecified reactive species

Narmeen Mallah,Maruxa Zapata-Cachafeiro,Carmelo Aguirre,Eguzkiñe Ibarra-García,Itziar Palacios-Zabalza,Fernando Macías-García,María Piñeiro-Lamas,Luisa Ibáñez,Xavier Vidal,Lourdes Vendrell,Luis Martin-Arias,María Sáinz-Gil,Verónica Velasco-González,Manuel Bacariza-Cortiñas,Angel Salgado,Ana Estany-Gestal,Adolfo Figueiras

Ecancermedicalscience 16:1351 PubMed35242232

2022

Evaluation of the association between gastric cancer and plasma selenium in Zambian adults: a case-control study.

Applications

Unspecified application

Species

Unspecified reactive species

Kanekwa Zyambo,Paul Kelly,Violet Kayamba

Scientific reports 11:19923 PubMed34620931

2021

A multicenter case-control study of the effect of e-nos VNTR polymorphism on upper gastrointestinal hemorrhage in NSAID users.

Applications

Unspecified application

Species

Unspecified reactive species

Narmeen Mallah,Maruxa Zapata-Cachafeiro,Carmelo Aguirre,Eguzkiñe Ibarra-García,Itziar Palacios-Zabalza,Fernando Macías García,Julio Iglesias García,María Piñeiro-Lamas,Luisa Ibáñez,Xavier Vidal,Lourdes Vendrell,Luis Martin-Arias,María Sáinz Gil,Verónica Velasco-González,Ángel Salgado-Barreira,Adolfo Figueiras

Scientific reports 10:15831 PubMed32985578

2020

Interaction between MyD88, TIRAP and IL1RL1 against Helicobacter pylori infection.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Fulgione,Marina Papaianni,Paola Cuomo,Debora Paris,Marco Romano,Concetta Tuccillo,Letizia Palomba,Chiara Medaglia,Massimiliano De Seta,Nicolino Esposito,Andrea Motta,Antonio Iannelli,Domenico Iannelli,Rosanna Capparelli

Frontiers in pharmacology 11:860 PubMed32655394

2020

Influence of Polymorphisms Involved in Platelet Activation and Inflammatory Response on Aspirin-Related Upper Gastrointestinal Bleeding: A Case-Control Study.

Applications

Unspecified application

Species

Unspecified reactive species

Narmeen Mallah,Maruxa Zapata-Cachafeiro,Carmelo Aguirre,Eguzkiñe Ibarra-García,Itziar Palacios-Zabalza,Fernando Macías-García,J Enrique Domínguez-Muñoz,María Piñeiro-Lamas,Luisa Ibáñez,Xavier Vidal,Lourdes Vendrell,Luis Martin-Arias,María Sáinz-Gil,Verónica Velasco-González,Adolfo Figueiras

FEBS letters 590:2127-37 PubMed27296467

2016

Epistatic interaction between MyD88 and TIRAP against Helicobacter pylori.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Fulgione,Antonio Di Matteo,Felice Contaldi,Rosanna Manco,Flora Ianniello,Guido Incerti,Massimiliano De Seta,Nicolino Esposito,Antonio Crasto,Domenico Iannelli,Rosanna Capparelli
View all publications
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