AB207621

Human Heme Oxygenase 1 ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(16 Publications)

Human Heme Oxygenase 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Heme Oxygenase 1 with a sensitivity of 7.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations

View Alternative Names

HO, HO1, HMOX1, Heme oxygenase 1, HO-1

10 Images

ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

ELISA

Supplier Data

ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

sELISA

Supplier Data

Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

Example of human Heme Oxygenase 1 standard curve in Sample Diluent 1X Extraction Buffer PTR. The Heme Oxygenase 1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heme Oxygenase 1 concentration was determined to be 1,630 pg/mL with a range of 391 – 3,198 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

Example of human Heme Oxygenase 1 standard curve in 1X Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

Example of human Heme Oxygenase 1 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

Interpolated concentrations of native Heme Oxygenase 1 in human liver homogenate extract and A549 cell extract based on a 15.6 μg/mL liver homogenate extract and 5 μg/mL A549 cell extract load.

The concentrations of Heme Oxygenase 1 were measured in duplicate and interpolated from the Heme Oxygenase 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heme Oxygenase 1 concentration was determined to be 3.30 ng/mL in liver homogenate extract and 1.60 ng/mL in A549 cell extract.

sELISA

Supplier Data

Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

Interpolated concentrations of native Heme Oxygenase 1 in human serum and plasma (EDTA) samples.

The concentrations of Heme Oxygenase 1 were measured in duplicate, interpolated from the Heme Oxygenase 1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heme Oxygenase 1 concentration was determined to be 1.37 ng/mL in serum and 2.12 ng/mL in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

Example of human Heme Oxygenase 1 standard curve in Sample Diluent NS. The Heme Oxygenase 1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

Linearity of dilution.

Native Heme Oxygenase 1 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native Heme Oxygenase 1 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 7.9 pg/mL

Range

23.44 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human Heme Oxygenase 1 ELISA Kit ab207621 is a rapid single-wash 90-min Sandwich ELISA to measure Human Heme Oxygenase 1 in cell culture extracts, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 7.9 pg/mL.

How the assay works

Human Heme Oxygenase 1 SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Heme Oxygenase 1 ELISA Kit ab207621 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Heme Oxygenase 1 antibody [EPR18689-5] - BSA and Azide free (Capture) ab242982
- Anti-Heme Oxygenase 1 antibody [EPR18689-126] - BSA and Azide free (Detector) ab242982

Heme oxygenase 1 (HO 1) is an enzyme that functions in heme catabolism. The activity of heme oxygenase 1 is to cleave the heme ring to form biliverdin. There are two isozymes of heme oxygenase that have 56% amino acid sequence identity: inducible heme oxygenase 1 and constitutively expressed heme oxygenase 2.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]

style

left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "76 - 88 %", "average": "= 83" }, { "sample": "Serum", "range": "79 - 94 %", "average": "= 87" }, { "sample": "EDTA Plasma", "range": "73 - 95 %", "average": "= 85" }, { "sample": "Cell culture extracts", "range": "76 - 88 %", "average": "= 83" }, { "sample": "Citrate plasma", "range": "77 - 93 %", "average": "= 85" }, { "sample": "Tissue Homogenate", "range": "86 - 92 %", "average": "= 90" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab207621-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB207621-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB207621-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB207621-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB207621-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB207621-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Heme Oxygenase 1 Lyophilized Recombinant Protein", "number":"AB207621-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human Heme Oxygenase 1 Capture Antibody (lyophilized)", "number":"AB207621-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB207621-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human Heme Oxygenase 1 Detector Antibody (lyophilized)", "number":"AB207621-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB207621-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB207621-CMP08", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heme oxygenase 1 (HO-1) also known as heme oxygenase-1 or oxygenase catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. It has been characterized with a mass of approximately 32 kDa. HO-1 is widely expressed in tissues with high metabolic activity such as liver spleen bone marrow and brain. This enzyme plays key roles in cellular response to oxidative stress and is responsive to various stress-inducing stimuli.

Biological function summary

The function of HO-1 involves important antioxidant defense mechanisms. The enzyme contributes to the cytoprotective effects by moderating oxidative stress and inflammation through its enzymatic activity. HO-1 often forms part of a larger regulatory complex where its activity regulates other cellular processes. Its heme byproducts biliverdin and bilirubin possess strong antioxidant properties adding to the cell's capacity to neutralize harmful oxidative effects.

Pathways

HO-1 operates in heme degradation and the antioxidative response pathway. It closely interacts with ferritin in its role of managing intracellular iron levels. As HO-1 breaks down heme the free iron released is sequestered by ferritin preventing cellular iron overload. HO-1 also plays a part in the signaling pathway involved in maintaining vascular homeostasis where it may modulate the activity of nitric oxide synthase through its byproducts.

Dysregulation of HO-1 levels connects to conditions such as cardiovascular diseases and certain neurodegenerative disorders. In cardiovascular diseases especially atherosclerosis the improper balance of heme oxygenase-1 activity can influence the severity of oxidative damage. Furthermore HO-1 relates to the pathogenesis of Alzheimer's disease where oxidative stress plays a significant role and where its interaction with proteins like amyloid-beta if disrupted may worsen disease pathology.

style

left-column

Product protocols

style

left-column

Target data

Heme oxygenase 1. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed : 11121422, PubMed : 19556236, PubMed : 7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed : 20055707).. Heme oxygenase 1. (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy.. Heme oxygenase 1 soluble form. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.

See full target information HMOX1

style

left-column

Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:20123 PubMed39209951

2024

Exploring the neuroprotective potential of Nrf2-pathway activators against annonacin toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Márcia F D Costa,Thomas W Rösler,Günter U Höglinger

BMC cardiovascular disorders 24:452 PubMed39192217

2024

Hemoglobin homeostasis in abdominal aortic aneurysm: diagnostic and prognostic potential of hemoglobin/heme and scavenger molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Sakshi Vats,Kristina Sundquist,Anton Grundberg,Jan Sundquist,Xiao Wang,Moncef Zarrouk,Anders Gottsäter,Ashfaque A Memon

NPJ Parkinson's disease 10:152 PubMed39174550

2024

Neuroprotection of low dose carbon monoxide in Parkinson's disease models commensurate with the reduced risk of Parkinson's among smokers.

Applications

Unspecified application

Species

Unspecified reactive species

K N Rose,M Zorlu,A Fassini,H Lee,W Cai,X Xue,S Lin,P Kivisakk,M A Schwarzschild,X Chen,S N Gomperts

Molecules (Basel, Switzerland) 29: PubMed38474603

2024

Comprehensive Transcriptome Profiling of Antioxidant Activities by Glutathione in Human HepG2 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshiaki Uchida,Farhana Ferdousi,Shinya Takahashi,Hiroko Isoda

International journal of molecular sciences 25: PubMed38339053

2024

Molecular Mechanisms and Therapeutic Implications of Human Pericyte-like Adipose-Derived Mesenchymal Stem Cells in an In Vitro Model of Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Aleksandra Agafonova,Alessia Cosentino,Ivana Roberta Romano,Giovanni Giurdanella,Floriana D'Angeli,Rosario Giuffrida,Debora Lo Furno,Carmelina Daniela Anfuso,Giuliana Mannino,Gabriella Lupo

International journal of molecular sciences 24: PubMed37685970

2023

Serum High-Mobility Group Box 1 and Heme Oxygenase-1 as Biomarkers in COVID-19 Patients at Hospital Admission.

Applications

Unspecified application

Species

Unspecified reactive species

Ilijana Grigorov,Snežana Pejić,Ana Todorović,Dunja Drakulić,Filip Veljković,Jadranka Miletić Vukajlović,Katarina Bobić,Ivan Soldatović,Siniša Đurašević,Nebojša Jasnić,Sanja Stanković,Sofija Glumac,Violeta Mihailović-Vučinić,Branislava Milenković

Antioxidants (Basel, Switzerland) 11: PubMed36290630

2022

Glucose Starvation-Caused Oxidative Stress Induces Inflammation and Autophagy in Human Gingival Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Runbo Li,Hirohito Kato,Yoichiro Taguchi,Xin Deng,Emika Minagawa,Takaya Nakata,Makoto Umeda

Frontiers in cellular and infection microbiology 12:1004148 PubMed36211962

2022

Heme oxygenase-1 modulates ferroptosis by fine-tuning levels of intracellular iron and reactive oxygen species of macrophages in response to infection.

Applications

Unspecified application

Species

Unspecified reactive species

Chenjie Ma,Xiaoling Wu,Xu Zhang,Xiaoming Liu,Guangcun Deng

Antioxidants (Basel, Switzerland) 11: PubMed36009215

2022

Beneficial Effects of Standardized Extracts from Wastes of Red Oranges and Olive Leaves.

Applications

Unspecified application

Species

Unspecified reactive species

Ilaria Burò,Valeria Consoli,Angela Castellano,Luca Vanella,Valeria Sorrenti

Free radical biology & medicine 188:105-116 PubMed35718303

2022

The role of cycloastragenol at the intersection of NRF2/ARE, telomerase, and proteasome activity.

Applications

Unspecified application

Species

Unspecified reactive species

Sinem Yilmaz,Erdal Bedir,Petek Ballar Kirmizibayrak

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Heme Oxygenase 1 ELISA Kit

ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Assay Kits

Product promise