Human Heme Oxygenase 1 ELISA Kit, 90-min ELISA (ab207621)

Human Heme Oxygenase 1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Heme Oxygenase 1 in Cell culture extracts, Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Tissue Extracts samples.

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Key facts

Detection method: Colorimetric
Sample types: Cell culture extracts, Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Tissue Extracts
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 23.44 - 1500 pg/mL
Assay time: 1h 30m
Sensitivity: = 7.9 pg/mL

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Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

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Target data

See full target information HMOX1

Function

Heme oxygenase 1. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed:11121422, PubMed:19556236, PubMed:7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed:20055707). Heme oxygenase 1. (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy. Heme oxygenase 1 soluble form. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.

Alternative names

HO, HO1, HMOX1, Heme oxygenase 1, HO-1

What's included?

1 x 96 Tests

Components

10X Wash Buffer PT (ab206977)

1 x 20 mL

5X Cell Extraction Buffer PTR (ab193970)

1 x 10 mL

Antibody Diluent 4BR

1 x 6 mL

Human Heme Oxygenase 1 Capture Antibody (lyophilized)

1 x 1 Vial

Human Heme Oxygenase 1 Detector Antibody (lyophilized)

1 x 1 Vial

Human Heme Oxygenase 1 Lyophilized Recombinant Protein

2 x 1 Vial

Plate Seals

1 x 1 Unit

Sample Diluent NS (ab193972)

1 x 50 mL

SimpleStep Pre-Coated 96-Well Microplate (ab206978)

1 x 1 Unit

Stop Solution

1 x 12 mL

TMB Development Solution

1 x 12 mL

Key facts

Detection method: Colorimetric
Sample types: Cell culture extracts, Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Tissue Extracts
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 23.44 - 1500 pg/mL
Assay time: 1h 30m
Assay Platform: Microplate (12 x 8 well strips)
Sensitivity: = 7.9 pg/mL

Precision

Intra assay

Sample	n	mean	SD	C.V.
Sample Serum	n 5	mean -	SD -	C.V. 3.1

Inter assay

Sample	n	mean	SD	C.V.
Sample Serum	n 3	mean -	SD -	C.V. 3.8

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Cell culture supernatant	Average % = 83	Range 76 - 88 %
Sample type Serum	Average % = 87	Range 79 - 94 %
Sample type EDTA Plasma	Average % = 85	Range 73 - 95 %
Sample type Cell culture extracts	Average % = 83	Range 76 - 88 %
Sample type Citrate plasma	Average % = 85	Range 77 - 93 %
Sample type Tissue Homogenate	Average % = 90	Range 86 - 92 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

Human Heme Oxygenase 1 ELISA Kit (ab207621) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Heme Oxygenase 1 protein in cell culture extracts, cell culture supernatant, serum, tissue extracts, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Heme Oxygenase 1 with 7.9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ASSAY SPECIFICITY

This kit recognizes both native and recombinant human Heme Oxygenase 1 protein in serum, plasma, cell culture supernatant, and cell and tissue extract samples.

SPECIES REACTIVITY

This kit recognizes human Heme Oxygenase 1 protein.
Other species reactivity was determined by measuring 50% (dilution) serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution. Reactivity < 3% was determined for the following species: Mouse , Rat , Hamster, Guinea Pig , Dog, Goat, Pig, Cow

Heme oxygenase 1 (HO 1) is an enzyme that functions in heme catabolism. The activity of heme oxygenase 1 is to cleave the heme ring to form biliverdin. There are two isozymes of heme oxygenase that have 56% amino acid sequence identity: inducible heme oxygenase 1 and constitutively expressed heme oxygenase 2.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Heme oxygenase 1 (HO-1) also known as heme oxygenase-1 or oxygenase catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. It has been characterized with a mass of approximately 32 kDa. HO-1 is widely expressed in tissues with high metabolic activity such as liver spleen bone marrow and brain. This enzyme plays key roles in cellular response to oxidative stress and is responsive to various stress-inducing stimuli.

Biological function summary

The function of HO-1 involves important antioxidant defense mechanisms. The enzyme contributes to the cytoprotective effects by moderating oxidative stress and inflammation through its enzymatic activity. HO-1 often forms part of a larger regulatory complex where its activity regulates other cellular processes. Its heme byproducts biliverdin and bilirubin possess strong antioxidant properties adding to the cell's capacity to neutralize harmful oxidative effects.

Pathways

HO-1 operates in heme degradation and the antioxidative response pathway. It closely interacts with ferritin in its role of managing intracellular iron levels. As HO-1 breaks down heme the free iron released is sequestered by ferritin preventing cellular iron overload. HO-1 also plays a part in the signaling pathway involved in maintaining vascular homeostasis where it may modulate the activity of nitric oxide synthase through its byproducts.

Associated diseases and disorders

Dysregulation of HO-1 levels connects to conditions such as cardiovascular diseases and certain neurodegenerative disorders. In cardiovascular diseases especially atherosclerosis the improper balance of heme oxygenase-1 activity can influence the severity of oxidative damage. Furthermore HO-1 relates to the pathogenesis of Alzheimer's disease where oxidative stress plays a significant role and where its interaction with proteins like amyloid-beta if disrupted may worsen disease pathology.

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9 product images

Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (ab207621)
Example of human Heme Oxygenase 1 standard curve in Sample Diluent 1X Extraction Buffer PTR. The Heme Oxygenase 1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (ab207621)
Example of human Heme Oxygenase 1 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (ab207621)
Example of human Heme Oxygenase 1 standard curve in 1X Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (ab207621)
Interpolated concentrations of native Heme Oxygenase 1 in human serum and plasma (EDTA) samples.
The concentrations of Heme Oxygenase 1 were measured in duplicate, interpolated from the Heme Oxygenase 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heme Oxygenase 1 concentration was determined to be 1.37 ng/mL in serum and 2.12 ng/mL in plasma (EDTA).
Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (ab207621)
Interpolated concentrations of native Heme Oxygenase 1 in human liver homogenate extract and A549 cell extract based on a 15.6 μg/mL liver homogenate extract and 5 μg/mL A549 cell extract load.
The concentrations of Heme Oxygenase 1 were measured in duplicate and interpolated from the Heme Oxygenase 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heme Oxygenase 1 concentration was determined to be 3.30 ng/mL in liver homogenate extract and 1.60 ng/mL in A549 cell extract.
Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (ab207621)
Example of human Heme Oxygenase 1 standard curve in Sample Diluent NS. The Heme Oxygenase 1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (ab207621)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heme Oxygenase 1 concentration was determined to be 1,630 pg/mL with a range of 391 – 3,198 pg/mL.
Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (ab207621)
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Heme Oxygenase 1 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (ab207621)
Linearity of dilution.
Native Heme Oxygenase 1 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent 1X Cell Extraction Buffer PTR.