Human Hemoglobin ELISA Kit

Product details

Human Hemoglobin ELISA Kit ab157707 is a sandwich ELISA to measure human hemoglobin in serum and plasma with a sensitivity of 0.8445 ng/ml.

How the assay works

In this assay the Hemoglobin present in samples reacts with the anti-Hemoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Hemoglobin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Hemoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hemoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hemoglobin in the test sample. The quantity of Hemoglobin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Assay specificity

Interferences : Azide and thimerosal at concentrations higher than 0.1% inhibits the enzyme reaction.

Human Hemoglobin ELISA Kit ab157707 protocol summary

1. Add samples and standards to wells. Incubate at room temperature.
2. Wash each well and add enzyme-antibody conjugate. Incubate at room temperature.
3. Wash each well and add Streptavidin Solution. Incubate at room temperature.
4. Add TMB Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read immediately.

How other researchers are using Human Hemoglobin ELISA kit ab157707

Human Hemoglobin ELISA kit ab157707 has been used to determine hemoglobin concentration cell free in neonates undergoing cardiopulmonary bypass ⁽¹⁾, and to study links between microglia and complement cascade in Huntingdon's disease ⁽²⁾.

References:

⁽¹⁾A. Jungner et al., 2022, PMID: 35887835,
⁽²⁾D.K Wilton et al. 2023, PMID:37814059

Related and recommended products

Human Hemoglobin ELISA kit 10157707 is commonly used for studying blood conditions such as anemia. Other ELISA kits used to measure blood proteins include :

- Human Erythropoietin ELISA kit ab274397
- Human Transferrin ELISA kit ab187391
- Human Haptoglobin ELISA kit ab309180