Human Hemopexin ELISA Kit
- Recombinant
- SimpleStep
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Human Hemopexin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Hemopexin with a sensitivity of 370 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Hemopexin, Beta-1B-glycoprotein, HPX
- ELISA
Supplier Data
ELISA - Human Hemopexin ELISA Kit (AB221838)
- ELISA
Supplier Data
ELISA - Human Hemopexin ELISA Kit (AB221838)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean hemopexin concentration was determined to be 1043 μg/mL with a range of 584 – 1316 μg/mL.
- ELISA
Supplier Data
ELISA - Human Hemopexin ELISA Kit (AB221838)
Example of human hemopexin standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
- ELISA
Supplier Data
ELISA - Human Hemopexin ELISA Kit (AB221838)
Interpolated concentrations of native hemopexin in human serum and plasma samples.
The concentrations of hemopexin were measured in duplicates, interpolated from the hemopexin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 40,000, plasma (citrate) 1 : 20,000, plasma (EDTA) 1 : 20,000, and plasma (heparin) 1 : 20,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean hemopexin concentration was determined to be 1004 μg/mL in serum, 768 μg/mL in plasma (citrate), 875 μg/mL in plasma (EDTA) and 812 μg/mL in plasma (heparin).
- ELISA
Supplier Data
ELISA - Human Hemopexin ELISA Kit (AB221838)
Interpolated concentrations of native hemopexin in human HepG2 cell culture supernatant samples.
The concentrations of hemopexin were measured in duplicates, interpolated from the hemopexin standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean hemopexin concentration was determined to be 24.3 ng/mL in HepG2 supernatant.
Reactivity data
Product details
Human Hemopexin ELISA Kit ab221838 is a rapid single-wash 90-min Sandwich ELISA to measure Human Hemopexin in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 370 pg/mL.
How the assay works
Human Hemopexin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Hemopexin ELISA Kit ab221838 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Hemopexin antibody [EPR18691-189] - BSA and Azide free (Capture) ab244914
- Anti-Hemopexin antibody [EPR18691-175] - BSA and Azide free (Detector) ab244914
Hemopexin is a heme-binding plasma glycoprotein which, after haptoglobin, forms the second line of defense against hemoglobin-mediated oxidative damage during intravascular hemolysis. A decrease in plasma hemopexin concentration reflects a recent release of heme compounds in the extracellular compartment. Heme–Hemopexin complexes are delivered to hepatocytes by receptor-mediated endocytosis after which Hemopexin is recycled to the circulation. Studies indicated that increased Hemopexin level associate with minimal change disease (MCD), sporadic Alzheimer's disease (AD), heavy-drinking chronic alcoholics, hemolytic anemias, chronic neuromuscular diseases and acute intermittent porphyria.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The hemopexin function relates to protecting tissues from the harmful effects of free heme. Hemopexin does not work as part of a complex in this process but mainly acts independently. Its high binding affinity plays an important role in maintaining heme homeostasis in the plasma and tissues. By controlling heme levels hemopexin supports overall cellular health and function.
Pathways
The hemopexin molecular weight allows it to fit effectively within the heme metabolism and iron recycling pathways. This protein plays a significant role by delivering heme to the liver and facilitating its degradation by heme oxygenase. Hemopexin interacts closely with heme oxygenase and albumin two proteins important for the management of heme and iron levels in the body.
Product protocols
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Target data
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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