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Human Hemopexin ELISA Kit ab221838 is a single-wash 90-min SimpleStep ELISA used to quantify Human Hemopexin with a sensitivity of 370 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.Easy-to-use, rapid, sensitive Human Hemopexin sandwich ELISA Kit:- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format- Colorimetric assay - 450nm readout; works on any plate reader- Looking for more flexibility? We also offer antibody pairs ready for conjugation

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Images

ELISA - Human Hemopexin ELISA Kit (AB221838), expandable thumbnail
  • ELISA - Human Hemopexin ELISA Kit (AB221838), expandable thumbnail
  • ELISA - Human Hemopexin ELISA Kit (AB221838), expandable thumbnail
  • ELISA - Human Hemopexin ELISA Kit (AB221838), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.78 - 50 ng/mL

Assay time

1h 30m

Sensitivity

= 370 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

1 product for Alternative Version

Target data

Function

Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.

Alternative names

What's included?

1 x 96 Tests
Components
10X Human Hemopexin Capture Antibody
1 x 600 µL
10X Human Hemopexin Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent 4BI
1 x 6 mL
Human Hemopexin Lyophilized Native Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human Hemopexin ELISA Kit ab221838 is a single-wash 90-min SimpleStep ELISA used to quantify Human Hemopexin with a sensitivity of 370 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.Easy-to-use, rapid, sensitive Human Hemopexin sandwich ELISA Kit:- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format- Colorimetric assay - 450nm readout; works on any plate reader- Looking for more flexibility? We also offer antibody pairs ready for conjugation

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.78 - 50 ng/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

Sensitivity

= 370 pg/mL

Precision

Intra assay

Sample

Serum

n
5
mean
-
SD
-
C.V.

3.3

Inter assay

Sample

Serum

n
3
mean
-
SD
-
C.V.

4.8

Recovery

Sample specific recovery

Sample type

Cell culture supernatant

Average %

= 107

Range

104 - 113 %

Sample type

Serum

Average %

= 108

Range

97 - 117 %

Sample type

EDTA Plasma

Average %

= 94

Range

85 - 103 %

Sample type

Heparin Plasma

Average %

= 105

Range

97 - 117 %

Sample type

Citrate plasma

Average %

= 97

Range

86 - 110 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human Hemopexin ELISA Kit (ab221838) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Hemopexin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Hemopexin with 370 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Hemopexin is a heme-binding plasma glycoprotein which, after haptoglobin, forms the second line of defense against hemoglobin-mediated oxidative damage during intravascular hemolysis. A decrease in plasma hemopexin concentration reflects a recent release of heme compounds in the extracellular compartment. Heme–Hemopexin complexes are delivered to hepatocytes by receptor-mediated endocytosis after which Hemopexin is recycled to the circulation. Studies indicated that increased Hemopexin level associate with minimal change disease (MCD), sporadic Alzheimer's disease (AD), heavy-drinking chronic alcoholics, hemolytic anemias, chronic neuromuscular diseases and acute intermittent porphyria.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Hemopexin also known as HPX is a glycoprotein that transports heme in the blood. It has a molecular weight of approximately 57 kDa and is primarily expressed in the liver. Hemopexin binds to free heme with high affinity which prevents oxidative damage and conserves iron. It transports the heme to hepatocytes for breakdown and recycling protecting cells from iron-mediated toxicity and oxidative stress.

Biological function summary

The hemopexin function relates to protecting tissues from the harmful effects of free heme. Hemopexin does not work as part of a complex in this process but mainly acts independently. Its high binding affinity plays an important role in maintaining heme homeostasis in the plasma and tissues. By controlling heme levels hemopexin supports overall cellular health and function.

Pathways

The hemopexin molecular weight allows it to fit effectively within the heme metabolism and iron recycling pathways. This protein plays a significant role by delivering heme to the liver and facilitating its degradation by heme oxygenase. Hemopexin interacts closely with heme oxygenase and albumin two proteins important for the management of heme and iron levels in the body.

Associated diseases and disorders

Several hemopexin functions contribute to the understanding of conditions such as hemolytic anemia and acute liver injury. In hemolytic anemia hemopexin helps mitigate the effects of increased heme levels due to excessive red blood cell breakdown. In the context of liver injury the protein assists in the detoxification process and supports hepatic function. Hemopexin-related proteins such as heme oxygenase link these diseases to hemopexin by their roles in managing oxidative stress and heme catabolism.

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4 product images

  • ELISA - Human Hemopexin ELISA Kit (ab221838), expandable thumbnail

    ELISA - Human Hemopexin ELISA Kit (ab221838)

    Example of human hemopexin standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • ELISA - Human Hemopexin ELISA Kit (ab221838), expandable thumbnail

    ELISA - Human Hemopexin ELISA Kit (ab221838)

    Interpolated concentrations of native hemopexin in human serum and plasma samples.

    The concentrations of hemopexin were measured in duplicates, interpolated from the hemopexin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:40,000, plasma (citrate) 1:20,000, plasma (EDTA) 1:20,000, and plasma (heparin) 1:20,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean hemopexin concentration was determined to be 1004 μg/mL in serum, 768 μg/mL in plasma (citrate), 875 μg/mL in plasma (EDTA) and 812 μg/mL in plasma (heparin).

  • ELISA - Human Hemopexin ELISA Kit (ab221838), expandable thumbnail

    ELISA - Human Hemopexin ELISA Kit (ab221838)

    Serum from ten individual healthy human female donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean hemopexin concentration was determined to be 1043 μg/mL with a range of 584 – 1316 μg/mL.

  • ELISA - Human Hemopexin ELISA Kit (ab221838), expandable thumbnail

    ELISA - Human Hemopexin ELISA Kit (ab221838)

    Interpolated concentrations of native hemopexin in human HepG2 cell culture supernatant samples.

    The concentrations of hemopexin were measured in duplicates, interpolated from the hemopexin standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean hemopexin concentration was determined to be 24.3 ng/mL in HepG2 supernatant.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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