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AB108859

Human Hemopexin ELISA Kit (HPX)

5

(3 Reviews)

|

(8 Publications)

Human Hemopexin ELISA Kit (HPX) is a competitive ELISA designed to quantify Human Hemopexin (HPX) with a sensitivity of 73 ng/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.078 - 20 µg/ml

View Alternative Names

Hemopexin, Beta-1B-glycoprotein, HPX

2 Images
Competitive ELISA - Human Hemopexin ELISA Kit (HPX) (AB108859)
  • cELISA

Supplier Data

Competitive ELISA - Human Hemopexin ELISA Kit (HPX) (AB108859)

Representative Standard Curve using ab108859.

Competitive ELISA - Human Hemopexin ELISA Kit (HPX) (AB108859)
  • cELISA

Lab

Competitive ELISA - Human Hemopexin ELISA Kit (HPX) (AB108859)

Hemopexin measured in various biological fluids showing quantity (μg) per mL of tested sample

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Competitive

Sensitivity

> 73 ng/mL

Range

0.078 - 20 µg/mL

Assay time

2h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Hemopexin (HPX) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of hemopexin levels in plasma and serum.

A Hemopexin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Hemopexin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Hemopexin captured in plate.

Get results in 90 minutes with Human Hemopexin ELISA Kit (ab171576) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108859-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"2X Biotinylated Human Hemopexin (Lyophilized)", "number":"AB108859-CMP10", "productcode":"" }, { "size":"1 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108859-CMP06", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108859-CMP07", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108859-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108859-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Hemopexin Microplate (12 x 8 well strips)", "number":"AB108859-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Hemopexin Standard", "number":"AB108859-CMP05", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108859-CMP08", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108859-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hemopexin also known as HPX is a glycoprotein that transports heme in the blood. It has a molecular weight of approximately 57 kDa and is primarily expressed in the liver. Hemopexin binds to free heme with high affinity which prevents oxidative damage and conserves iron. It transports the heme to hepatocytes for breakdown and recycling protecting cells from iron-mediated toxicity and oxidative stress.
Biological function summary

The hemopexin function relates to protecting tissues from the harmful effects of free heme. Hemopexin does not work as part of a complex in this process but mainly acts independently. Its high binding affinity plays an important role in maintaining heme homeostasis in the plasma and tissues. By controlling heme levels hemopexin supports overall cellular health and function.

Pathways

The hemopexin molecular weight allows it to fit effectively within the heme metabolism and iron recycling pathways. This protein plays a significant role by delivering heme to the liver and facilitating its degradation by heme oxygenase. Hemopexin interacts closely with heme oxygenase and albumin two proteins important for the management of heme and iron levels in the body.

Several hemopexin functions contribute to the understanding of conditions such as hemolytic anemia and acute liver injury. In hemolytic anemia hemopexin helps mitigate the effects of increased heme levels due to excessive red blood cell breakdown. In the context of liver injury the protein assists in the detoxification process and supports hepatic function. Hemopexin-related proteins such as heme oxygenase link these diseases to hemopexin by their roles in managing oxidative stress and heme catabolism.
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Product protocols

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Target data

Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
See full target information Hemopexin
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

BMC cardiovascular disorders 24:452 PubMed39192217

2024

Hemoglobin homeostasis in abdominal aortic aneurysm: diagnostic and prognostic potential of hemoglobin/heme and scavenger molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Sakshi Vats,Kristina Sundquist,Anton Grundberg,Jan Sundquist,Xiao Wang,Moncef Zarrouk,Anders Gottsäter,Ashfaque A Memon

Pulmonary circulation 13:e12185 PubMed36743426

2023

Transpulmonary generation of cell-free hemoglobin contributes to vascular dysfunction in pulmonary arterial hypertension via dysregulated clearance mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Jamie E Meegan,Vern Eric Kerchberger,Niki L Fortune,Joel Brennan McNeil,Julie A Bastarache,Eric D Austin,Lorraine B Ware,Anna R Hemnes,Evan L Brittain

Science advances 8:eadc9245 PubMed36563141

2022

Circulating hemopexin modulates anthracycline cardiac toxicity in patients and in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Liu,Sarah Lane,Rahul Lall,Michele Russo,Laurie Farrell,Melis Debreli Coskun,Casie Curtin,Raquel Araujo-Gutierrez,Marielle Scherrer-Crosbie,Barry H Trachtenberg,Jonghan Kim,Emanuela Tolosano,Alessandra Ghigo,Robert E Gerszten,Aarti Asnani

Cell reports 32:108181 PubMed32966797

2020

Scavenging of Labile Heme by Hemopexin Is a Key Checkpoint in Cancer Growth and Metastases.

Applications

Unspecified application

Species

Unspecified reactive species

Giacomo Canesin,Annalisa Di Ruscio,Mailin Li,Simone Ummarino,Andreas Hedblom,Reeham Choudhury,Agnieszka Krzyzanowska,Eva Csizmadia,Macarena Palominos,Anna Stiehm,Alexander Ebralidze,Shao-Yong Chen,Mahmoud A Bassal,Ping Zhao,Emanuela Tolosano,Laurence Hurley,Anders Bjartell,Daniel G Tenen,Barbara Wegiel

Functional & integrative genomics 20:191-200 PubMed31444657

2019

Plasma protein(s)-based conceptual diagnostic tool for assessing high-altitude acclimation in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Subhojit Paul,Anamika Gangwar,Kalpana Bhargava,Nilofar Khan,Pankaj Khurana,Yasmin Ahmad

International journal of obesity (2005) 41:631-638 PubMed28119529

2017

Physiologic and genetic evidence links hemopexin to triglycerides in mice and humans.

Applications

Unspecified application

Species

Unspecified reactive species

H A Lawson,M Zayed,J P Wayhart,E Fabbrini,L Love-Gregory,S Klein,C F Semenkovich

Haematologica 100:1508-16 PubMed26315932

2015

Impairment of neutrophil oxidative burst in children with sickle cell disease is associated with heme oxygenase-1.

Applications

Unspecified application

Species

Unspecified reactive species

Ceri Evans,Katharine Orf,Erzsebet Horvath,Michael Levin,Josu De La Fuente,Subarna Chakravorty,Aubrey J Cunnington

PloS one 9:e100902 PubMed24979072

2014

Identification of reduced circulating haptoglobin concentration as a biomarker of the severity of pulmonary embolism: a nontargeted proteomic study.

Applications

Unspecified application

Species

Unspecified reactive species

María Insenser,Rafael Montes-Nieto,M Ángeles Martínez-García,Elena Fernandez Durán,Carmen Santiuste,Vicente Gómez,Jeffrey A Kline,Héctor F Escobar-Morreale,David Jiménez
View all publications
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