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AB108860

Human Hemopexin ELISA Kit (HPX)

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(3 Publications)

Human Hemopexin ELISA Kit (HPX) is a Sandwich (quantitative) ELISA for the measurement of Human Hemopexin (HPX) in Human in Biofluids samples.

View Alternative Names

Hemopexin, Beta-1B-glycoprotein, HPX

2 Images
Sandwich ELISA - Human Hemopexin ELISA Kit (HPX) (AB108860)
  • sELISA

Unknown

Sandwich ELISA - Human Hemopexin ELISA Kit (HPX) (AB108860)

Hemopexin measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample. Human saliva diluted 60-120 fold. Human milk diluted 300-600 fold. Human urine diluted 10 fold - below detection. Cell supernatants tested neat.

Sandwich ELISA - Human Hemopexin ELISA Kit (HPX) (AB108860)
  • sELISA

Supplier Data

Sandwich ELISA - Human Hemopexin ELISA Kit (HPX) (AB108860)

Representative Standard Curve using ab108860.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Saliva, Urine, Milk

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.1 ng/mL

Range

6.5 - 400 ng/mL

Assay time

3h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Hemopexin (HPX) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Hemopexin levels in Human CSF, milk, saliva and urine.

A Hemopexin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Hemopexin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Hemopexin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108860-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108860-CMP06", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108860-CMP07", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108860-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108860-CMP08", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Hemopexin Antibody", "number":"AB108860-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Hemopexin Microplate (12 x 8 well strips)", "number":"AB108860-CMP03", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108860-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Hemopexin Standard", "number":"AB108860-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108860-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hemopexin also known as HPX is a glycoprotein that transports heme in the blood. It has a molecular weight of approximately 57 kDa and is primarily expressed in the liver. Hemopexin binds to free heme with high affinity which prevents oxidative damage and conserves iron. It transports the heme to hepatocytes for breakdown and recycling protecting cells from iron-mediated toxicity and oxidative stress.
Biological function summary

The hemopexin function relates to protecting tissues from the harmful effects of free heme. Hemopexin does not work as part of a complex in this process but mainly acts independently. Its high binding affinity plays an important role in maintaining heme homeostasis in the plasma and tissues. By controlling heme levels hemopexin supports overall cellular health and function.

Pathways

The hemopexin molecular weight allows it to fit effectively within the heme metabolism and iron recycling pathways. This protein plays a significant role by delivering heme to the liver and facilitating its degradation by heme oxygenase. Hemopexin interacts closely with heme oxygenase and albumin two proteins important for the management of heme and iron levels in the body.

Several hemopexin functions contribute to the understanding of conditions such as hemolytic anemia and acute liver injury. In hemolytic anemia hemopexin helps mitigate the effects of increased heme levels due to excessive red blood cell breakdown. In the context of liver injury the protein assists in the detoxification process and supports hepatic function. Hemopexin-related proteins such as heme oxygenase link these diseases to hemopexin by their roles in managing oxidative stress and heme catabolism.
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Product protocols

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Target data

Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
See full target information Hemopexin
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1369385 PubMed38915417

2024

Longitudinal assessment of urinary ALCAM, HPX, and PRDX6 in Korean patients with systemic lupus erythematosus: implications for disease activity monitoring and treatment response.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Won Kim,Wook-Young Baek,Ju-Yang Jung,Hyoun-Ah Kim,Sang-Won Lee,Chang-Hee Suh

Journal of Cancer 8:3631-3640 PubMed29151949

2017

Cerebrospinal Fluid Proteins Identification Facilitates the Differential Diagnosis of Central Nervous System Diffuse Large B Cell Lymphoma.

Applications

FuncS

Species

Unspecified reactive species

Wen Zheng,YuQin Song,Yan Xie,NingJing Lin,MeiFeng Tu,WeiPing Liu,LingYan Ping,ZhiTao Ying,Chen Zhang,LiJuan Deng,XiaoPei Wang,YouYong Lu,Jun Zhu

American journal of physiology. Renal physiology 303:F1460-72 PubMed22993068

2012

Renal cortical hemopexin accumulation in response to acute kidney injury.

Applications

sELISA

Species

Unspecified reactive species

Richard A Zager,Ali C M Johnson,Kirsten Becker
View all publications
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websiteProtocolBooklet
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