Human Heparanase ELISA Kit (HPSE) is a single-wash 90-min Simplestep used to quantify Human Heparanase (HPSE) with a sensitivity of 67.76 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
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- EBioMedicine 90:1045062023Peroxisome proliferator-activated receptor ɣ agonist mediated inhibition of heparanase expression reduces proteinuria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarjolein Garsen,Baranca Buijsers,Marloes Sol,Lena Gockeln,Ramon Sonneveld,Toin H van Kuppevelt,Mark de Graaf,Jacob van den Born,Jan A A M Kamps,Daniël H van Raalte,Rutger W van der Meer,Hildo J Lamb,Jan-Luuk Hillebrands,Ton J Rabelink,Marissa L Maciej-Hulme,Guido Krenning,Tom Nijenhuis,Johan van der VlagPubMed 36889064
- Biomedicines 10:2022Anticancer Effects with Molecular Docking Confirmation of Newly Synthesized Isatin Sulfonamide Molecular Hybrid Derivatives against Hepatic Cancer Cell Lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMahmoud Eldeeb,Eman F Sanad,Ahmed Ragab,Yousry A Ammar,Khaled Mahmoud,Mamdouh M Ali,Nadia M HamdyPubMed 35327524
- Frontiers in immunology 12:7595702021Increased Plasma Heparanase Activity and Endothelial Glycocalyx Degradation in Dengue Patients Is Associated With Plasma Leakage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaranca Buijsers,Fadel Muhammad Garishah,Silvita Fitri Riswari,Rosalie M van Ast,Setyo Gundi Pramudo,Rahajeng N Tunjungputri,Gijs J Overheul,Ronald P van Rij,André van der Ven,Bachti Alisjahbana,Muhammad Hussein Gasem,Quirijn de Mast,Johan van der VlagPubMed 34987504
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 125 - 8000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 67.76 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as a procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.
Alternative names
HEP, HPA, HPA1, HPR1, HPSE1, HSE1, HPSE, Heparanase, Endo-glucoronidase, Heparanase-1, Hpa1
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Human Heparanase ELISA Kit (HPSE) is a single-wash 90-min Simplestep used to quantify Human Heparanase (HPSE) with a sensitivity of 67.76 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 125 - 8000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 67.76 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 8 | mean - | SD - | C.V. 4.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 91 | Range |
Sample type EDTA Plasma | Average % = 96 | Range |
Sample type Tissue Extracts | Average % = 104 | Range |
Sample type Citrate plasma | Average % = 83 | Range |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Heparanase ELISA Kit (HPSE) (ab256401) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Heparanase (HPSE) protein in cit plasma, edta plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Heparanase (HPSE) with 67.76 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human Heparanase is an endoglycosidase which is a cell surface and extracellular matrix-degrading enzyme. It cleaves heparan sulfate proteoglycans into heparan sulfate side chains and core proteoglycans. Heparanase is also implicated in the extravasation of leukocytes and tumor cell lines. Due to its contribution to metastasis and angiogenesis, Heparanase is considered as a potential target for anti-cancer therapies. It is highly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues. Human Heparanase shares 75 % sequence homology with mouse and rat.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Heparanase 1 also known as HPA1 or HPSE is an endo-β-D-glucuronidase enzyme with a molecular mass of approximately 50 kDa. It functions mechanically by cleaving heparan sulfate (HS) chains which are long sugar chains found on the extracellular matrix and cell surfaces. This cleavage activity remodels the matrix and influences cell behavior. Heparanase 1 is mainly expressed in the placenta lymphoid tissues and various tumor cells where it plays a role in matrix degradation and cell migration.
- Biological function summary
When heparan sulfate is cleaved by Heparanase 1 it causes the release of HS-bound growth factors and cytokines making them available to cells. This enzymatic activity facilitates cell proliferation and angiogenesis affecting processes important for tissue repair and cancer progression. Heparanase 1 does not require a complex for its function but operates as a monomeric enzyme. Its activity is investigated using assays like heparanase activity assays or ELISA for measuring the enzyme's activity in different biological contexts including mouse models.
- Pathways
The activity of Heparanase 1 intersects significantly with the regulation of angiogenesis and cell signaling pathways such as the VEGF and FGF pathways. These pathways are important for new blood vessel formation making them significant in both normal development and cancer. Heparanase 1 interacts with proteins such as VEGF which is important for blood vessel growth and integrins which are involved in cell-matrix interactions and signaling.
- Associated diseases and disorders
Heparanase 1 is associated with tumor metastasis and inflammation. Its ability to degrade the extracellular matrix enables cancer cell invasion and metastasis. The enzyme also plays a role in inflammatory diseases where it modulates the immune response. Through these disease pathways it relates to other proteins such as matrix metalloproteinases (MMPs) which also participate in matrix remodeling during tumor progression and inflammation.
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3 product images
Sandwich ELISA - Human Heparanase ELISA Kit (HPSE) (ab256401)
Example of human Heparanase standard curve in Sample Diluent NS.
The Heparanase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Heparanase ELISA Kit (HPSE) (ab256401)
Interpolated concentrations of native Heparanase in human serum and plasma samples.
The concentrations of Heparanase were measured in duplicates, interpolated from the Heparanase standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 12.5%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heparanase concentration was determined to be 5,242 pg/mL in serum, 6,728 pg/mL in plasma (citrate) and 2,493 pg/mL in plasma (EDTA).
Sandwich ELISA - Human Heparanase ELISA Kit (HPSE) (ab256401)
Interpolated concentrations of native Heparanase in human spleen extract based on a 250 μg/mL extract load.
The concentrations of Heparanase were measured in duplicate and interpolated from the Heparanase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heparanase concentration was determined to be 3,599 pg/mL in spleen extract.
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