AB256401

Human Heparanase ELISA Kit (HPSE)

Recombinant
SimpleStep
What is this?

(1 Review)

(5 Publications)

Human Heparanase ELISA Kit (HPSE) is a single-wash 90-min Simplestep used to quantify Human Heparanase (HPSE) with a sensitivity of 67.76 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

HEP, HPA, HPA1, HPR1, HPSE1, HSE1, HPSE, Heparanase, Endo-glucoronidase, Heparanase-1, Hpa1

3 Images

Sandwich ELISA - Human Heparanase ELISA Kit (HPSE) (AB256401)

sELISA

Supplier Data

Sandwich ELISA - Human Heparanase ELISA Kit (HPSE) (AB256401)

Example of human Heparanase standard curve in Sample Diluent NS.

The Heparanase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Heparanase ELISA Kit (HPSE) (AB256401)

Interpolated concentrations of native Heparanase in human serum and plasma samples.

The concentrations of Heparanase were measured in duplicates, interpolated from the Heparanase standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (citrate) 12.5%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heparanase concentration was determined to be 5,242 pg/mL in serum, 6,728 pg/mL in plasma (citrate) and 2,493 pg/mL in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Human Heparanase ELISA Kit (HPSE) (AB256401)

Interpolated concentrations of native Heparanase in human spleen extract based on a 250 μg/mL extract load.

The concentrations of Heparanase were measured in duplicate and interpolated from the Heparanase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heparanase concentration was determined to be 3,599 pg/mL in spleen extract.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 67.76 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human Heparanase ELISA Kit (HPSE) (ab256401) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Heparanase (HPSE) protein in cit plasma, edta plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Heparanase (HPSE) with 67.76 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Human Heparanase is an endoglycosidase which is a cell surface and extracellular matrix-degrading enzyme. It cleaves heparan sulfate proteoglycans into heparan sulfate side chains and core proteoglycans. Heparanase is also implicated in the extravasation of leukocytes and tumor cell lines. Due to its contribution to metastasis and angiogenesis, Heparanase is considered as a potential target for anti-cancer therapies. It is highly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues. Human Heparanase shares 75 % sequence homology with mouse and rat.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": null, "average": "= 91" }, { "sample": "EDTA Plasma", "range": null, "average": "= 96" }, { "sample": "Tissue Extracts", "range": null, "average": "= 104" }, { "sample": "Citrate plasma", "range": null, "average": "= 83" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab256401-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB256401-CMP12", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB256401-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB256401-CMP13", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Heparanase (HPSE) Lyophilized Recombinant Protein", "number":"AB256401-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Heparanase (HPSE) Capture Antibody", "number":"AB256401-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB256401-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB256401-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB256401-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB256401-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"10X Human Heparanase (HPSE) Detector Antibody", "number":"AB256401-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB256401-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB256401-CMP07", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heparanase 1 also known as HPA1 or HPSE is an endo-β-D-glucuronidase enzyme with a molecular mass of approximately 50 kDa. It functions mechanically by cleaving heparan sulfate (HS) chains which are long sugar chains found on the extracellular matrix and cell surfaces. This cleavage activity remodels the matrix and influences cell behavior. Heparanase 1 is mainly expressed in the placenta lymphoid tissues and various tumor cells where it plays a role in matrix degradation and cell migration.

Biological function summary

When heparan sulfate is cleaved by Heparanase 1 it causes the release of HS-bound growth factors and cytokines making them available to cells. This enzymatic activity facilitates cell proliferation and angiogenesis affecting processes important for tissue repair and cancer progression. Heparanase 1 does not require a complex for its function but operates as a monomeric enzyme. Its activity is investigated using assays like heparanase activity assays or ELISA for measuring the enzyme's activity in different biological contexts including mouse models.

Pathways

The activity of Heparanase 1 intersects significantly with the regulation of angiogenesis and cell signaling pathways such as the VEGF and FGF pathways. These pathways are important for new blood vessel formation making them significant in both normal development and cancer. Heparanase 1 interacts with proteins such as VEGF which is important for blood vessel growth and integrins which are involved in cell-matrix interactions and signaling.

Heparanase 1 is associated with tumor metastasis and inflammation. Its ability to degrade the extracellular matrix enables cancer cell invasion and metastasis. The enzyme also plays a role in inflammatory diseases where it modulates the immune response. Through these disease pathways it relates to other proteins such as matrix metalloproteinases (MMPs) which also participate in matrix remodeling during tumor progression and inflammation.

style

left-column

Product protocols

style

left-column

Target data

Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as a procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.

See full target information HPSE

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Breast cancer research : BCR 26:153 PubMed39506780

2024

The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jasmine M Manouchehri,Jharna Datta,Lynn M Marcho,Jesse J Reardon,Daniel Stover,Robert Wesolowski,Uma Borate,Ting-Yuan David Cheng,Patrick M Schnell,Bhuvaneswari Ramaswamy,Gina M Sizemore,Mark P Rubinstein,Mathew A Cherian

iScience 27:108930 PubMed38333700

2024

Serum proteome profiling reveals heparanase as a candidate biomarker for chronic thromboembolic pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Yunxia Zhang,Meng Zhang,Hongwei Yang,Haobo Li,Shuangshuang Ma,Linfeng Xi,Yishan Li,Xincheng Li,Zhihui Fu,Zhu Zhang,Shuai Zhang,Qian Gao,Qiang Huang,Jun Wan,Wanmu Xie,Jifeng Li,Peiran Yang,Zhenguo Zhai

EBioMedicine 90:104506 PubMed36889064

2023

Peroxisome proliferator-activated receptor ɣ agonist mediated inhibition of heparanase expression reduces proteinuria.

Applications

Unspecified application

Species

Unspecified reactive species

Marjolein Garsen,Baranca Buijsers,Marloes Sol,Lena Gockeln,Ramon Sonneveld,Toin H van Kuppevelt,Mark de Graaf,Jacob van den Born,Jan A A M Kamps,Daniël H van Raalte,Rutger W van der Meer,Hildo J Lamb,Jan-Luuk Hillebrands,Ton J Rabelink,Marissa L Maciej-Hulme,Guido Krenning,Tom Nijenhuis,Johan van der Vlag

Biomedicines 10: PubMed35327524

2022

Anticancer Effects with Molecular Docking Confirmation of Newly Synthesized Isatin Sulfonamide Molecular Hybrid Derivatives against Hepatic Cancer Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Mahmoud Eldeeb,Eman F Sanad,Ahmed Ragab,Yousry A Ammar,Khaled Mahmoud,Mamdouh M Ali,Nadia M Hamdy

Frontiers in immunology 12:759570 PubMed34987504

2021

Increased Plasma Heparanase Activity and Endothelial Glycocalyx Degradation in Dengue Patients Is Associated With Plasma Leakage.

Applications

Unspecified application

Species

Unspecified reactive species

Baranca Buijsers,Fadel Muhammad Garishah,Silvita Fitri Riswari,Rosalie M van Ast,Setyo Gundi Pramudo,Rahajeng N Tunjungputri,Gijs J Overheul,Ronald P van Rij,André van der Ven,Bachti Alisjahbana,Muhammad Hussein Gasem,Quirijn de Mast,Johan van der Vlag

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Heparanase ELISA Kit (HPSE)

Sandwich ELISA - Human Heparanase ELISA Kit (HPSE) (AB256401)

Sandwich ELISA - Human Heparanase ELISA Kit (HPSE) (AB256401)

Sandwich ELISA - Human Heparanase ELISA Kit (HPSE) (AB256401)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Secondary Antibodies

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise