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AB326895

Human Heparanase (HPSE) ELISA Kit, Chemiluminescent

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Human Heparanase (HPSE) ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Heparanase (HPSE) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 18.29 - 40000 pg/ml with a sensitivity of 5.01 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

HEP, HPA, HPA1, HPR1, HPSE1, HSE1, HPSE, Heparanase, Endo-glucoronidase, Heparanase-1, Hpa1

4 Images
Sandwich ELISA - Human Heparanase (HPSE) ELISA Kit, Chemiluminescent (AB326895)
  • sELISA

Lab

Sandwich ELISA - Human Heparanase (HPSE) ELISA Kit, Chemiluminescent (AB326895)

Dynamic range comparison between the Chemiluminescent Human Heparanase SimpleStep ELISA and colorimetric Human Heparanase SimpleStep ELISA (ab256401). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human Heparanase (HPSE) ELISA Kit, Chemiluminescent (AB326895)
  • sELISA

Lab

Sandwich ELISA - Human Heparanase (HPSE) ELISA Kit, Chemiluminescent (AB326895)

Standard curve comparison between the Chemiluminescent Human Heparanase SimpleStep ELISA and colorimetric Human Heparanase SimpleStep ELISA (ab256401) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Heparanase (HPSE) ELISA Kit, Chemiluminescent (AB326895)
  • sELISA

Supplier Data

Sandwich ELISA - Human Heparanase (HPSE) ELISA Kit, Chemiluminescent (AB326895)

Interpolated concentrations of native Heparanase in human spleen extract based on a 250 μg/mL extract load.

The concentrations of Heparanase were measured in duplicate and interpolated from the Heparanase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heparanase concentration was determined to be 3,599 pg/mL in spleen extract.

Sandwich ELISA - Human Heparanase (HPSE) ELISA Kit, Chemiluminescent (AB326895)
  • sELISA

Supplier Data

Sandwich ELISA - Human Heparanase (HPSE) ELISA Kit, Chemiluminescent (AB326895)

Interpolated concentrations of native Heparanase in human serum and plasma samples.

The concentrations of Heparanase were measured in duplicates, interpolated from the Heparanase standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (citrate) 12.5%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heparanase concentration was determined to be 5,242 pg/mL in serum, 6,728 pg/mL in plasma (citrate) and 2,493 pg/mL in plasma (EDTA).

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Tissue Extracts, Citrate plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.01 pg/mL

Range

18.29 - 40000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human Heparanase (HPSE) ELISA Kit, Chemiluminescent (ab326895) is a rapid single-wash sandwich 55-min ELISA to measure Human Heparanase (HPSE) in Serum, EDTA Plasma, Tissue Extracts, Citrate plasma with a sensitivity of 5.01 pg/mL.

How the assay works

Human Heparanase (HPSE) ELISA Kit, Chemiluminescent (ab326895) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Heparanase (HPSE) (ab326895) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Heparanase antibody [EPR22365-202] - BSA and Aside free (Capture) ab259443
- Anti-Heparanase antibody [EPR22365-218] - BSA and Aside free (Detector) ab259444
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Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.5" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as a procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.
See full target information HPSE
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websiteProtocolBooklet
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Product promise

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