Human Hexokinase II ELISA Kit

• sELISA

Supplier Data

Sandwich ELISA - Human Hexokinase II ELISA Kit (AB219043)

Example of human Hexokinase II standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

• sELISA

Supplier Data

Sandwich ELISA - Human Hexokinase II ELISA Kit (AB219043)

Interpolated concentrations of native Hexokinase II in human placenta, HeLa, and Jurkat extract samples based on a 50 μg/mL extract load.

The concentrations of Hexokinase II were measured in duplicate and interpolated from the Hexokinase II standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Hexokinase II concentration was determined to be 1,175.25 pg/mL in placenta extract, 2,844.72 pg/mL in HeLa extract, and 2,577.06 pg/mL in Jurkat extract.

• sELISA

Supplier Data

Sandwich ELISA - Human Hexokinase II ELISA Kit (AB219043)

Interpolated concentrations of native Hexokinase II in human fetal brain, mouse brain, and rat brain extract samples based on 25 μg/mL, 100 μg/mL, and 12.5 μg/mL extract loads, respectively.

The concentrations of Hexokinase II were measured in duplicate and interpolated from the Hexokinase II standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Hexokinase II concentration was determined to be 624.25 pg/mL in human fetal brain extract, 4,691.00 pg/mL in mouse brain extract, and 973.64 pg/mL in rat brain extract.

• sELISA

Supplier Data

Sandwich ELISA - Human Hexokinase II ELISA Kit (AB219043)

Representative image of Human Hexokinase II ELISA Kit (ab219043)

Components shown from left to right :

- Sample diluent NS

- 10X Wash Buffer PT (ab206977)

- 10X Human Hexokinase Detector Antibody

- Human Hexokinase Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Human Hexokinase Capture Antibody (Lyophilised)

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent CPI2

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

• sELISA

Supplier Data

Sandwich ELISA - Human Hexokinase II ELISA Kit (AB219043)

Interpolated concentrations of spiked Hexokinase II in human serum and plasma samples.

The concentrations of Hexokinase II were measured in duplicates, interpolated from the Hexokinase II standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Hexokinase II concentration was determined to be 7,009.26 pg/mL in neat serum, 7,887.82 pg/mL in neat plasma (citrate), 8,052.65 pg/mL in neat plasma (EDTA), and 8,675.63 pg/mL in neat plasma (heparin).

• sELISA

Supplier Data

Sandwich ELISA - Human Hexokinase II ELISA Kit (AB219043)

Example of human Hexokinase II standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.