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AB275901

Human HGF ELISA Kit (Hepatocyte Growth Factor)

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(7 Publications)

Human HGF ELISA Kit (Hepatocyte Growth Factor) is a single-wash 90-min Simplestep used to quantify Human HGF (Hepatocyte Growth Factor) with a sensitivity of 18 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

HPTA, HGF, Hepatocyte growth factor, Hepatopoietin-A, Scatter factor, SF

7 Images
ELISA - Human HGF ELISA Kit (Hepatocyte Growth Factor) (AB275901)
  • ELISA

Supplier Data

ELISA - Human HGF ELISA Kit (Hepatocyte Growth Factor) (AB275901)
Sandwich ELISA - Human HGF ELISA Kit (Hepatocyte Growth Factor) (AB275901)
  • sELISA

Supplier Data

Sandwich ELISA - Human HGF ELISA Kit (Hepatocyte Growth Factor) (AB275901)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 151 pg/mL with a range of undetectable to 242 pg/mL.

Sandwich ELISA - Human HGF ELISA Kit (Hepatocyte Growth Factor) (AB275901)
  • sELISA

Supplier Data

Sandwich ELISA - Human HGF ELISA Kit (Hepatocyte Growth Factor) (AB275901)

Human HGF competitor curve comparison.

Standard Curve comparison between Human HGF SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human HGF ELISA Kit (Hepatocyte Growth Factor) (AB275901)
  • sELISA

Supplier Data

Sandwich ELISA - Human HGF ELISA Kit (Hepatocyte Growth Factor) (AB275901)

Interpolated concentrations of native HGF in neat human serum and plasma samples.

The concentrations of HGF were measured in duplicates in neat samples, interpolated from the HGF standard curves and plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 129 pg/mL in neat serum, 154 pg/mL in neat plasma (citrate) and 158 pg/mL in neat plasma (heparin).

Sandwich ELISA - Human HGF ELISA Kit (Hepatocyte Growth Factor) (AB275901)
  • sELISA

Supplier Data

Sandwich ELISA - Human HGF ELISA Kit (Hepatocyte Growth Factor) (AB275901)

Example of human HGF standard curve in Sample Diluent 25BP.

The HGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human HGF ELISA Kit (Hepatocyte Growth Factor) (AB275901)
  • sELISA

Supplier Data

Sandwich ELISA - Human HGF ELISA Kit (Hepatocyte Growth Factor) (AB275901)

Interpolated concentrations of native HGF in human A-204 cell and placenta tissue based on 100 μg/mL and 200 µg/mL extract loads, respectively.

The concentrations of HGF were measured in duplicate and interpolated from the HGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 4,077 pg/mL in A-204 extract and 2,681 pg/mL in placenta extract.

Sandwich ELISA - Human HGF ELISA Kit (Hepatocyte Growth Factor) (AB275901)
  • sELISA

Supplier Data

Sandwich ELISA - Human HGF ELISA Kit (Hepatocyte Growth Factor) (AB275901)

Example of human HGF standard curve in 1X Cell Extraction Buffer PTRThe HGF standard curve was prepared as described in Section 10.

The HGF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 18 pg/mL

Range

78.13 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human HGF ELISA Kit (Hepatocyte Growth Factor) ab275901 is a rapid single-wash 90-min Sandwich ELISA to measure Human HGF (Hepatocyte Growth Factor)in cell culture media, cell lysate, citrate plasma, heparin plasma, serum. This SimpleStep sensitivity is 18 pg/mL.

How the assay works

Human HGF (Hepatocyte Growth Factor)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human HGF ELISA Kit (Hepatocyte Growth Factor) ab275901 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-HGF antibody [EPR22870-72] - BSA and Azide free (Capture) ab276168
- Anti-HGF antibody [EPR22870-13] - BSA and Azide free (Detector) ab276168

Hepatocyte Growth Factor (HGF) is mitogen of parenchymal hepatocytes. HGF also acts as a growth factor and is expressed by a wide variety of tissue and cell types. HGF binds to the HGF- Receptor (MET), a receptor tyrosine kinase, to help regulate cell growth, cell motility and morphogenesis. Elevated circulating levels of HGF can be measured as prognostic marker of severity of hypertension and heart failure. HGF is secreted as a full-length inactive polypeptide, and then is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta chain which dimerize producing the active heterodimeric molecule.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" } ]
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Recovery

[ { "sample": "Serum", "range": "86 - 97 %", "average": "= 92" }, { "sample": "Cell culture media", "range": "103 - 107 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "75 - 80 %", "average": "= 77" }, { "sample": "Cell Lysate", "range": "77 - 84 %", "average": "= 81" }, { "sample": "Citrate plasma", "range": "81 - 83 %", "average": "= 82" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab275901-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB275901-CMP14", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB275901-CMP13", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human HGF (Hepatocyte Growth Factor) Capture Antibody", "number":"AB275901-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB275901-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human HGF (Hepatocyte Growth Factor) Lyophilized Recombinant Protein", "number":"AB275901-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BP", "number":"AB275901-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB275901-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB275901-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB275901-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB275901-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human HGF (Hepatocyte Growth Factor) Detector Antibody", "number":"AB275901-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB275901-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The hepatocyte growth factor (HGF) also known as scatter factor (SF) is a protein with a mass of approximately 83 kDa. This protein is mostly expressed in the liver but it can also be found in other organs such as the kidney and the lungs. HGF acts mechanically by binding to the c-Met receptor activating intracellular signaling mechanisms necessary for various physiological processes. Researchers often use HGF ELISA kits to measure its levels in biological samples and study its various roles.
Biological function summary

HGF plays an essential role in cellular growth regeneration and healing processes across different tissues. It is not part of a complex but functions as a single-chain polypeptide that undergoes cleavage into its active form. HGF facilitates cell proliferation and migration; this is especially important during organ regeneration and repair processes. By engaging with its receptor c-Met it induces cell survival and cytoskeletal reorganization making it important for wound healing.

Pathways

HGF is critical in the MAPK and PI3K-Akt signaling pathways which impact processes like cell division and survival. Through these pathways HGF interacts with other proteins such as ERK and Akt contributing to the regulation of cellular growth and metabolism. HGF's role in these pathways highlights its importance in developmental processes and tissue homeostasis.

HGF displays significant connections to cancer and liver cirrhosis. Its overexpression can lead to the development of various carcinomas due to its effect on cell proliferation and migration. In liver cirrhosis the altered HGF-c-Met signaling might contribute to the disease progression and hepatic dysfunction. Additionally HGF can interact with proteins such as transforming growth factor-beta (TGF-?) in these conditions influencing the pathological environment.
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Product protocols

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Target data

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types (PubMed : 20624990). Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (PubMed : 15167892, PubMed : 20977675). Activates MAPK signaling following TMPRSS13 cleavage and activation (PubMed : 20977675).
See full target information HGF
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 13:1550447 PubMed40496140

2025

Mesenchymal stem cell-derived exosome subpopulations remained consistent for 28 culture days, displaying therapeutic effects in a silicosis mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Lina Zhang,Jing Jin,Liguang Sun,Gang Hou,Mingming Deng,Yiding Bian,Jianming Liu,Wei Cheng,Shaoliang Xing,Wenjia Wang,Xin Dong,Qingjie Fan,Lei Gao,Xinhua Lei,Yongli Bao,Yongguang Yang

Cell transplantation 34:9636897241301703 PubMed39831589

2025

Tumor Necrosis Factor Receptor 1 Is Required for Human Umbilical Cord-Derived Mesenchymal Stem Cell-Mediated Rheumatoid Arthritis Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Guangyang Liu,Herui Wang,Chenliang Zhang,Xin Li,Yi Mi,Yaoyao Chen,Liqiang Xu,Li Miao,Haomiao Long,Yongjun Liu

Cancer immunology, immunotherapy : CII 74:58 PubMed39751636

2025

Role of the HGF/c-MET pathway in resistance to immune checkpoint inhibitors in advanced non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Assya Akli,Paul Takam Kamga,Catherine Julie,Claude Capron,Adrien Costantini,Coraline Dumenil,Jennifer Dumoulin,Violaine Giraud,Florence Parent,Andrei Seferian,Catherine Guettier,Mathieu Glorion,Elisabeth Longchampt,Jean-François Emile,Étienne Giroux-Leprieur

Nature communications 15:5010 PubMed38866762

2024

Transient growth factor expression via mRNA in lipid nanoparticles promotes hepatocyte cell therapy in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Anna R Smith,Fatima Rizvi,Elissa Everton,Anisah Adeagbo,Susan Wu,Ying Tam,Hiromi Muramatsu,Norbert Pardi,Drew Weissman,Valerie Gouon-Evans

Experimental and therapeutic medicine 27:182 PubMed38515646

2024

Mesenchymal stem cells derived from CHIR99021 and TGF‑β induction remained on the colicomentum and improved cardiac function of a rat model of acute myocardium infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Yusen Zhang,Yanmin Zhang,Azhen Hu,Fanhua Meng,Peng Cui,Tianshi Li,Guanghui Cui

Journal of functional biomaterials 15: PubMed38248687

2024

Development of a Nanoparticle System for Controlled Release in Bioprinted Respiratory Scaffolds.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda Zimmerling,Christina Sunil,Yan Zhou,Xiongbiao Chen

Nature communications 14:7639 PubMed37993431

2023

Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Ye He,Shujie Yang,Pengzhan Liu,Ke Li,Ke Jin,Ryan Becker,Jinxin Zhang,Chuanchuan Lin,Jianping Xia,Zhehan Ma,Zhiteng Ma,Ruoyu Zhong,Luke P Lee,Tony Jun Huang
View all publications
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