AB227898

Human HIF-2-alpha ELISA Kit

Recombinant
SimpleStep
What is this?

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(4 Publications)

Human HIF-2-alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify Human HIF-2-alpha with a sensitivity of 19 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

BHLHE73, HIF2A, MOP2, PASD2, EPAS1, Endothelial PAS domain-containing protein 1, EPAS-1, Basic-helix-loop-helix-PAS protein MOP2, Class E basic helix-loop-helix protein 73, HIF-1-alpha-like factor, Hypoxia-inducible factor 2-alpha, Member of PAS protein 2, PAS domain-containing protein 2, bHLHe73, HLF, HIF-2-alpha, HIF2-alpha

3 Images

ELISA - Human HIF-2-alpha ELISA Kit (AB227898)

ELISA

Supplier Data

ELISA - Human HIF-2-alpha ELISA Kit (AB227898)

Example of Human HIF-2-alpha standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

ELISA

Supplier Data

ELISA - Human HIF-2-alpha ELISA Kit (AB227898)

Interpolated concentrations of native HIF-2-alpha in DFO treated Human HeL1 cell extract, sample based on a 250 μg/mL extract load.

HELA cells were untreated or treated with 250 μM DFO for 24 hrs, then collected and extracted according to sample preparation protocol (see section 11.1). The concentrations of HIF-2-alpha were measured in duplicate and interpolated from the HIF-2-alpha standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HIF-2-alpha concentration was determined to be 520 pg/mL in DFO treated HELA cell extract and undetectable in untreated HELA cell extract.

ELISA

Supplier Data

ELISA - Human HIF-2-alpha ELISA Kit (AB227898)

Dose-dependent induction of HIF-2-alpha in HELA cells by deferoxamine (DFO).

HeLa cells were cultured in 96-well tissue culture plates and were either untreated (Vehicle Control) or exposed to varying doses of DFO for 24 hours (DFO treated). Cells were extracted directly in the culture plate by overlaying culture media with Cell Extraction Buffer PTR with Enhancer such that the final concentration was 1X Cell Extraction Buffer PTR. Extracts were applied to the HIF-2-alpha SimpleStep ELISA plate. Raw data and standard deviation is plotted from quadruplicate measurements.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 19 pg/mL

Range

31.3 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human HIF-2-alpha ELISA Kit (ab227898) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of HIF-2-alpha protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human HIF-2-alpha with 19 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "HeLa Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" }, { "reproducibilityType": "Intra", "sample": "HeLa Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" } ]

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Recovery

[ { "sample": "Cell culture extracts", "range": "112 - 116 %", "average": "= 114" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab227898-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB227898-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB227898-CMP13", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human HIF-2-alpha Lyophilized Recombinant Protein", "number":"AB227898-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB227898-CMP14", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB227898-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human HIF-2-alpha Capture Antibody", "number":"AB227898-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB227898-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB227898-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB227898-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB227898-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human HIF-2-alpha Detector Antibody", "number":"AB227898-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB227898-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HIF-2-alpha also known as hypoxia-inducible factor 2-alpha or EPAS1 plays a significant role in cellular response to oxygen levels. Mechanically HIF-2-alpha functions as a transcription factor that activates certain genes when oxygen is low. The protein typically weighs around 118 kDa. It is expressed in various tissues but particularly in endothelial cells and some neuronal tissues. These sites highlight its importance in organs requiring tight regulation of oxygen.

Biological function summary

HIF-2-alpha regulates the expression of genes involved in energy metabolism and angiogenesis. The protein forms a complex by dimerizing with the HIF-1-beta subunit which is necessary for transcriptional activity. Through this complex formation and activity it influences processes such as erythropoiesis and regulates factors like vascular endothelial growth factor (VEGF). Therefore HIF-2-alpha contributes to the adaptation of cells and tissues under hypoxic conditions.

Pathways

HIF-2-alpha engages in the hypoxia signaling pathway playing an essential part by modulating gene expression in response to low oxygen availability. This role impacts other proteins such as HIF-1-alpha sharing overlapping functions but with distinct target genes. Additionally HIF-2-alpha is involved in the mTOR pathway which influences cell growth and metabolism through its interaction with the nutrient-sensing regulatory pathway.

Researchers have linked HIF-2-alpha with conditions like renal cell carcinoma and pulmonary hypertension. The protein's dysregulation can lead to altered expression of angiogenic factors promoting tumor growth and survival. In particular its connection with VEGF exemplifies its role in these pathologies by facilitating aberrant blood vessel formation. Such interactions further highlight the potential of HIF-2-alpha as a therapeutic target in disease modulation.

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Product protocols

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Target data

Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX1 seems to activate CTAD (By similarity).

See full target information EPAS1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pediatrics 10:919879 PubMed35958170

2022

Decreased plasma levels of PDGF-BB, VEGF-A, and HIF-2α in preterm infants after ibuprofen treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Xuemei Huang,Dongshan Han,Yanfei Wei,Bingchun Lin,Dingyuan Zeng,Yu Zhang,Ba Wei,Zhifeng Huang,Xueyu Chen,Chuanzhong Yang

Frontiers in chemistry 10:890675 PubMed35518717

2022

Phthalimide Analogs Enhance Genotoxicity of Cyclophosphamide and Inhibit Its Associated Hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Amira M Gamal-Eldeen,Hussein S Agwa,Magdy A-H Zahran,Bassem M Raafat,Sherien M El-Daly,Hamsa J Banjer,Mazen M Almehmadi,Afaf Alharthi,Nahed M Hawsawi,Fayez Althobaiti,Mona A M Abo-Zeid

Frontiers in pharmacology 13:860898 PubMed35401227

2022

Perftoran Inhibits Hypoxia-Associated Resistance in Lung Cancer Cells to Carboplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Amira M Gamal-Eldeen,Amani A Alrehaili,Afaf Alharthi,Bassem M Raafat

Annals of translational medicine 10:223 PubMed35280389

2022

Identification of differentially expressed and methylated genes and construction of a co-expression network in age-related macular degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Gaohua Liang,Wenhao Ma,Yanni Luo,Jiayang Yin,Lili Hao,Jingxiang Zhong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human HIF-2-alpha ELISA Kit

ELISA - Human HIF-2-alpha ELISA Kit (AB227898)

ELISA - Human HIF-2-alpha ELISA Kit (AB227898)

ELISA - Human HIF-2-alpha ELISA Kit (AB227898)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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