AB223593

Human HLA-DR ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Human HLA-DR ELISA Kit is a single-wash 90-min Simplestep used to quantify Human HLA-DR with a sensitivity of 5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

HLA-DRA1, HLA-DRA, MHC class II antigen DRA

4 Images

ELISA - Human HLA-DR ELISA Kit (AB223593)

ELISA

Supplier Data

ELISA - Human HLA-DR ELISA Kit (AB223593)

sELISA

Supplier Data

Sandwich ELISA - Human HLA-DR ELISA Kit (AB223593)

Representative image of Human HLA-DR ELISA Kit (ab223593)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human HLA-DR ELISA Kit Capture Antibody

- 10X Human HLA-DR Detector Antibody

- 10X Human HLA-DR ELISA Kit Lyophilised Purified Protein (2 vials)

- Human HLA-DR ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Human HLA-DR ELISA Kit (AB223593)

Interpolated concentrations of native HLA-DR in human cell and tissue extract samples.

The concentrations of HLA-DR were measured in duplicates, interpolated from the HLA-DR standard curves corrected for sample dilution and extract load. Undiluted samples are as follows : Daudi cell extract 32.3 μg/mL, Raji cell extract 490 μg/mL, and human skin extract 500 μg/mL. The interpolated dilution factor and corrected values are plotted (mean +/- SD, n=2). The mean HLA-DR concentration was determined to be 52.0 pg/μg in Daudi Extract, 1.93 pg/μg in Raji Extract and 0.85 pg/μg in human skin homogenate extract.

sELISA

Supplier Data

Sandwich ELISA - Human HLA-DR ELISA Kit (AB223593)

Example of human HLA-DR standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Homogenate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 5 pg/mL

Range

31.3 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human HLA-DR ELISA Kit ab223593 is a rapid single-wash 90-min Sandwich ELISA to measure Human HLA-DR in cell culture extracts, tissue homogenate. This SimpleStep sensitivity is 5 pg/mL.

How the assay works

Human HLA-DR SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human HLA-DR ELISA Kit ab223593 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-HLA-DR antibody [EPR20209-156] - BSA and Azide free (Capture) ab242928
- Anti-HLA-DR antibody [EPR20209-171] - BSA and Azide free (Detector) ab242928

HLA class II histocompatibility antigen, DR alpha chain (HLA-DR) is the alpha chain of a cell surface receptor heterodimer that binds peptides for presentation to CD4 T cells. The alpha chain is fairly constant between individuals and pairs with varying alleles of the beta chain. HLA-DR has been implicated in multiple auto-immune conditions, as well as graft vs host disease.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12.7" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]

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Recovery

[ { "sample": "Cell culture extracts", "range": "89 - 105 %", "average": "= 98" }, { "sample": "Tissue Homogenate", "range": "86 - 100 %", "average": "= 94" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab223593-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB223593-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB223593-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB223593-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB223593-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB223593-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB223593-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human HLA-DR Lyophilized Recombinant Protein", "number":"AB223593-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB223593-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human HLA-DR Capture Antibody", "number":"AB223593-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB223593-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB223593-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human HLA-DR Detector Antibody", "number":"AB223593-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DR-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells (PubMed : 15265931, PubMed : 15322540, PubMed : 17334368, PubMed : 22327072, PubMed : 24190431, PubMed : 27591323, PubMed : 29884618, PubMed : 31495665, PubMed : 8145819, PubMed : 9075930). Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes (PubMed : 8145819). In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins (PubMed : 31495665). Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance (PubMed : 17182262, PubMed : 23783831). The selection of the immunodominant epitopes follows two processing modes : 'bind first, cut/trim later' for pathogen-derived antigenic peptides and 'cut first, bind later' for autoantigens/self-peptides (PubMed : 25413013). The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules (PubMed : 8145819).

See full target information HLA-DRA

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of tissue engineering and regenerative medicine 16:290-296 PubMed34965018

2022

Demineralized bone matrix combined with cytotoxic T-lymphocyte-associated protein 4 promotes osteogenic differentiation of human bone marrow mesenchymal stem cells and suppresses the activation of T lymphocytes in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Song,Rui Zhou,Jun Xiao,Lei He,Fang Zhu,Congcan Li,Fei Dai

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human HLA-DR ELISA Kit

ELISA - Human HLA-DR ELISA Kit (AB223593)

Sandwich ELISA - Human HLA-DR ELISA Kit (AB223593)

Sandwich ELISA - Human HLA-DR ELISA Kit (AB223593)

Sandwich ELISA - Human HLA-DR ELISA Kit (AB223593)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

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