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AB235652

Human HNRNPK ELISA Kit

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Human HNRNPK ELISA Kit is a single-wash 90-min Simplestep used to quantify Human HNRNPK with a sensitivity of 84 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

HNRPK, HNRNPK, Heterogeneous nuclear ribonucleoprotein K, hnRNP K, Transformation up-regulated nuclear protein, TUNP

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Sandwich ELISA - Human HNRNPK ELISA Kit (AB235652)
  • sELISA

Supplier Data

Sandwich ELISA - Human HNRNPK ELISA Kit (AB235652)

Representative image of Human HNRNPK ELISA Kit (ab235652)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human HNRNPK ELISA Kit Capture Antibody

- 10X Human HNRNPK Detector Antibody

- 10X Human HNRNPK ELISA Kit Lyophilised Purified Protein (2 vials)

- Human HNRNPK ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human HNRNPK ELISA Kit (AB235652)
  • sELISA

Supplier Data

Sandwich ELISA - Human HNRNPK ELISA Kit (AB235652)

Interpolated concentrations of native HNRNPK in MCF-7 extract and Raw264.7 extract based on a 12.5 μg/mL and 12.5 μg/mL extract loads, respectively.

The concentrations of HNRNPK were measured in duplicate and interpolated from the HNRNPK standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HNRNPK concentration was determined to be 2,847 pg/mL in MCF-7 extract and 1,384 pg/mL in Raw264.7 extract.

Sandwich ELISA - Human HNRNPK ELISA Kit (AB235652)
  • sELISA

Supplier Data

Sandwich ELISA - Human HNRNPK ELISA Kit (AB235652)

Example of human HNRNPK standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 84 pg/mL

Range

117.19 - 7500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human HNRNPK ELISA Kit (ab235652) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of HNRNPK protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human HNRNPK with 84 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": null, "average": "= 120" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab235652-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB235652-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB235652-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB235652-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB235652-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB235652-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB235652-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human HNRNPK Lyophilized Recombinant Protein", "number":"AB235652-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human HNRNPK Capture Antibody", "number":"AB235652-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB235652-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB235652-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB235652-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human HNRNPK Detector Antibody", "number":"AB235652-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a multifunctional protein that plays a critical role in RNA metabolism including RNA splicing translation and transcription. Alternate names for hnRNP K include synkaryon-associated protein and hnRNP KurB. It has a molecular mass of approximately 51 kDa and is ubiquitously expressed in the nucleus and cytoplasm of eukaryotic cells. This protein features three KH (K homology) domains that enable it to interact with nucleic acids and other proteins.
Biological function summary

HnRNP K serves as a transcriptional regulator and participates in the assembly of ribonucleoprotein complexes. As part of the ribonucleoprotein complex it interacts with both RNA and DNA influencing various gene expression pathways. It also modulates the activity of several transcription factors including c-Myc and has been implicated in cell proliferation and differentiation. This protein's versatile functions make it an important component in various cellular processes.

Pathways

HnRNP K plays an important role in signal transduction and the p53 pathway. In the p53 pathway hnRNP K acts as a coactivator enhancing the transcriptional activity of the tumor suppressor p53 and influencing cell cycle regulation and apoptosis. Additionally hnRNP K interacts with several proteins including the c-Myc oncogene to regulate gene expression associated with growth and cell cycle progression.

HnRNP K has been linked to cancer and neurological disorders. Its interaction with c-Myc makes it relevant in various cancers where c-Myc is dysregulated contributing to unchecked cell proliferation. Furthermore alterations in hnRNP K expression or function have been associated with neurodegenerative disorders where it might interact with proteins like TDP-43 or FUS affecting neuronal function and survival.
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Product protocols

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Target data

One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest. As part of a ribonucleoprotein complex composed at least of ZNF827, HNRNPL and the circular RNA circZNF827 that nucleates the complex on chromatin, may negatively regulate the transcription of genes involved in neuronal differentiation (PubMed : 33174841).
See full target information HNRNPK
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websiteProtocolBooklet
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