AB324417

Human HRG ELISA Kit

SimpleStep
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Human HRG ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human HRG in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Histidine-rich glycoprotein, Histidine-proline-rich glycoprotein, HPRG, HRG

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Sandwich ELISA - Human HRG ELISA Kit (AB324417)

sELISA

Lab

Sandwich ELISA - Human HRG ELISA Kit (AB324417)

Interpolated concentration of native HRG was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 160,000 human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human HRG ELISA Kit (AB324417)

Example of human HRG standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human HRG ELISA Kit (AB324417)

Example of human HRG standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human HRG ELISA Kit (AB324417)

Interpolated concentration of native HRG was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 20 milk. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human HRG ELISA Kit (AB324417)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean HRG concentration was determined to be 116.5 µg/mL with a range of 85.0 – 175.4 µg/mL.

sELISA

Lab

Sandwich ELISA - Human HRG ELISA Kit (AB324417)

Interpolated concentration of native HRG was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 800,000 serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Milk

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 15.03 pg/mL

Range

39.063 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human HRG ELISA Kit is a single-wash 90-min Simplestep used to quantify Human HRG with a sensitivity of 15.03pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

- Different format for different needs: 10 x 96 plates for bulk orders and 384-well for higher throughput.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
- Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.7" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.4" } ]

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Recovery

[ { "sample": "Serum", "range": "102 - 117 %", "average": "= 111" }, { "sample": "Citrate plasma", "range": "107 - 119 %", "average": "= 114" }, { "sample": "EDTA Plasma", "range": "94 - 109 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "106 - 121 %", "average": "= 114" }, { "sample": "Cell culture media", "range": "95 - 106 %", "average": "= 101" }, { "sample": "Milk", "range": "86 - 91 %", "average": "= 88" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Plasma glycoprotein that binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Binds heparin and heparin/glycosaminoglycans in a zinc-dependent manner. Binds heparan sulfate on the surface of liver, lung, kidney and heart endothelial cells. Binds to N-sulfated polysaccharide chains on the surface of liver endothelial cells. Inhibits rosette formation. Acts as an adapter protein and is implicated in regulating many processes such as immune complex and pathogen clearance, cell chemotaxis, cell adhesion, angiogenesis, coagulation and fibrinolysis. Mediates clearance of necrotic cells through enhancing the phagocytosis of necrotic cells in a heparan sulfate-dependent pathway. This process can be regulated by the presence of certain HRG ligands such as heparin and zinc ions. Binds to IgG subclasses of immunoglobins containing kappa and lambda light chains with different affinities regulating their clearance and inhibiting the formation of insoluble immune complexes. Tethers plasminogen to the cell surface. Binds T-cells and alters the cell morphology. Modulates angiogenesis by blocking the CD6-mediated antiangiongenic effect of thrombospondins, THBS1 and THBS2. Acts as a regulator of the vascular endothelial growth factor (VEGF) signaling pathway; inhibits endothelial cell motility by reducing VEGF-induced complex formation between PXN/paxillin and ILK/integrin-linked protein kinase and by promoting inhibition of VEGF-induced tyrosine phosphorylation of focal adhesion kinases and alpha-actinins in endothelial cells. Also plays a role in the regulation of tumor angiogenesis and tumor immune surveillance. Normalizes tumor vessels and promotes antitumor immunity by polarizing tumor-associated macrophages, leading to decreased tumor growth and metastasis.

See full target information Histidine-rich glycoprotein

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