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Human HSD1 ELISA kit is a Sandwich (quantitative) ELISA kit for the measurement of Human HSD1 in Human in Cell culture supernatant, Plasma, Serum samples.

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Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.9 - 200 ng/mL

Sensitivity

= 0.9 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Favors the reduction of estrogens and androgens. Converts estrone (E1) to a more potent estrogen, 17beta-estradiol (E2) (PubMed:8994190). Also has 20-alpha-HSD activity. Uses preferentially NADH.

Alternative names

What's included?

1 x 96 Tests
Components
200X HRP-Streptavidin
1 x 200 µL
20X Wash Buffer
1 x 25 mL
5X Assay Diluent B (Item E)
1 x 15 mL
5X Assay Diluent D (Item K)
1 x 15 mL
Biotinylated Human HSD1 Detection Antibody
2 x 1 Vial
Human HSD1 Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human HSD1 Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

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Human HSD1 ELISA kit is a Sandwich (quantitative) ELISA kit for the measurement of Human HSD1 in Human in Cell culture supernatant, Plasma, Serum samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.9 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 0.9 ng/mL

Recovery

Sample specific recovery

Sample type

Cell culture media

Average %

= 81.7

Range

94 - 104 %

Sample type

Plasma

Average %

= 85.69

Range

76 - 93 %

Sample type

Serum

Average %

= 93.37

Range

90 - 97 %

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Human HSD1 (also known as HSD17B1) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human HSD1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human HSD1 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTSL-1/Punctin-1, AMSH/STAMBP, Annexin V, BATF3, Bora, Cadherin-17, Caveolin-2, CD2, CD200 R1, CHST3, COMT, Cystatin SA, Dopamine beta-Hydroxylase, Desmin, Exostosin-like 3, Ficolin-1, FosB/GOS3, FRS2, GATA-5, GFAP, GLI-3, HepaCAM, ARNT/HIF1 beta, IDO, Kallikrein 1, Kell, MDL-1/CLEC5A, NPDC-1, Numb, Olig2, p63/TP73L, Pax3, Semaphorin 4D, Sphingosine Kinase 1/SPHK1, TAZ/WWTR1, TC-PTP, Transglutaminase 3/TGM3, TPST2, TREML1/TLT-1.

Supplementary info

Biological function summary

Hsd17b11 influences steroidal balance by regulating the levels of active and inactive steroid hormones. It is sometimes associated with lipid metabolism processes but remains independent not a part of any known enzymatic complex. By controlling hormone levels Hsd17b11 impacts cellular functions related to metabolism growth and reproduction reinforcing its importance in maintaining proper physiological conditions.

Activity summary

Hsd17b11 also known as type 11 17-beta hydroxysteroid dehydrogenase is an enzyme that plays a role in steroid metabolism by catalyzing the conversion of less active 17-keto steroids to more active 17-beta-hydroxy steroids. The dehydrogenase weighs approximately 34 kDa and is found in various tissues but it is highly expressed in the liver and other steroidogenic tissues. As an NAD(P)-dependent oxidoreductase it contributes to maintaining steroid homeostasis within the cellular environment.

Pathways

Hsd17b11 participates in steroid hormone metabolism and has roles within the broader steroidogenic pathway. It is related to the regulation of sex steroids closely working with enzymes like Hsd17b3 and Hsd17b2 which also modulate steroid hormones. These interactions contribute to comprehensive steroid biosynthesis and degradation pathways forming a network critical for endocrine system health.

Associated diseases and disorders

Several studies have linked Hsd17b11 to disorders of steroid metabolism including polycystic ovary syndrome (PCOS) and some liver dysfunctions. Changes in its activity can affect steroid hormone levels perturbed in PCOS which often associates with aberrant androgen production. In metabolic liver disorders altered Hsd17b11 activity may disrupt lipid metabolism. The enzyme may also interact indirectly with proteins like SHBG (sex hormone-binding globulin) in these contexts influencing disease pathology and presentation.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human HSD1 ELISA kit (ab313502), expandable thumbnail

    Sandwich ELISA - Human HSD1 ELISA kit (ab313502)

    Example of standard curve using ab313502. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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