JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB313502

Human HSD1 ELISA kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human HSD1 ELISA kit is a Sandwich ELISA for the measurement of Human HSD1 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

E17KSR, EDH17B1, EDH17B2, EDHB17, SDR28C1, HSD17B1, 17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1, 20 alpha-hydroxysteroid dehydrogenase, E2DH, Estradiol 17-beta-dehydrogenase 1, Placental 17-beta-hydroxysteroid dehydrogenase, Short chain dehydrogenase/reductase family 28C member 1, 20-alpha-HSD

1 Images
Sandwich ELISA - Human HSD1 ELISA kit (AB313502)
  • sELISA

Supplier Data

Sandwich ELISA - Human HSD1 ELISA kit (AB313502)

Example of standard curve using ab313502. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.9 ng/mL

Range

0.9 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Primary Antibodies

AB224437

Anti-Hsd17b11 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsd17b11 antibody (AB224437)

  • 0
  • 0
Proteins & Peptides

AB101826

Recombinant Human Hsd17b11 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Hsd17b11 protein (His tag N-Terminus) (AB101826)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human HSD1 (also known as HSD17B1) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human HSD1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human HSD1 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTSL-1/Punctin-1, AMSH/STAMBP, Annexin V, BATF3, Bora, Cadherin-17, Caveolin-2, CD2, CD200 R1, CHST3, COMT, Cystatin SA, Dopamine beta-Hydroxylase, Desmin, Exostosin-like 3, Ficolin-1, FosB/GOS3, FRS2, GATA-5, GFAP, GLI-3, HepaCAM, ARNT/HIF1 beta, IDO, Kallikrein 1, Kell, MDL-1/CLEC5A, NPDC-1, Numb, Olig2, p63/TP73L, Pax3, Semaphorin 4D, Sphingosine Kinase 1/SPHK1, TAZ/WWTR1, TC-PTP, Transglutaminase 3/TGM3, TPST2, TREML1/TLT-1.

style
left-column

Recovery

[ { "sample": "Cell culture media", "range": "94 - 104 %", "average": "= 81.7" }, { "sample": "Plasma", "range": "76 - 93 %", "average": "= 85.69" }, { "sample": "Serum", "range": "90 - 97 %", "average": "= 93.37" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313502-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313502-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313502-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313502-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human HSD1 Detection Antibody", "number":"AB313502-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB313502-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313502-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human HSD1 Antibody-coated ELISA Plate", "number":"AB313502-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human HSD1 Protein Standard", "number":"AB313502-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB313502-CMP06", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsd17b11 also known as type 11 17-beta hydroxysteroid dehydrogenase is an enzyme that plays a role in steroid metabolism by catalyzing the conversion of less active 17-keto steroids to more active 17-beta-hydroxy steroids. The dehydrogenase weighs approximately 34 kDa and is found in various tissues but it is highly expressed in the liver and other steroidogenic tissues. As an NAD(P)-dependent oxidoreductase it contributes to maintaining steroid homeostasis within the cellular environment.
Biological function summary

Hsd17b11 influences steroidal balance by regulating the levels of active and inactive steroid hormones. It is sometimes associated with lipid metabolism processes but remains independent not a part of any known enzymatic complex. By controlling hormone levels Hsd17b11 impacts cellular functions related to metabolism growth and reproduction reinforcing its importance in maintaining proper physiological conditions.

Pathways

Hsd17b11 participates in steroid hormone metabolism and has roles within the broader steroidogenic pathway. It is related to the regulation of sex steroids closely working with enzymes like Hsd17b3 and Hsd17b2 which also modulate steroid hormones. These interactions contribute to comprehensive steroid biosynthesis and degradation pathways forming a network critical for endocrine system health.

Several studies have linked Hsd17b11 to disorders of steroid metabolism including polycystic ovary syndrome (PCOS) and some liver dysfunctions. Changes in its activity can affect steroid hormone levels perturbed in PCOS which often associates with aberrant androgen production. In metabolic liver disorders altered Hsd17b11 activity may disrupt lipid metabolism. The enzyme may also interact indirectly with proteins like SHBG (sex hormone-binding globulin) in these contexts influencing disease pathology and presentation.
style
left-column

Product protocols

style
left-column

Target data

Favors the reduction of estrogens and androgens. Converts estrone (E1) to a more potent estrogen, 17beta-estradiol (E2) (PubMed : 8994190). Also has 20-alpha-HSD activity. Uses preferentially NADH.
See full target information HSD17B1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com