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AB313972

Human HSD17B14 ELISA Kit

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Human HSD17B14 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human HSD17B14 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

DHRS10, SDR3, SDR47C1, UNQ502/PRO474, HSD17B14, 17-beta-hydroxysteroid dehydrogenase 14, 17-beta-HSD 14, 17-beta-hydroxysteroid dehydrogenase DHRS10, Dehydrogenase/reductase SDR family member 10, Retinal short-chain dehydrogenase/reductase retSDR3, Short chain dehydrogenase/reductase family 47C member 1

1 Images
Sandwich ELISA - Human HSD17B14 ELISA Kit (AB313972)
  • sELISA

Supplier Data

Sandwich ELISA - Human HSD17B14 ELISA Kit (AB313972)

Example of standard curve using ab313972. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 22 pg/mL

Range

22 - 5000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Human HSD17B14 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human HSD17B14 in serum (human HSD17B14 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human HSD17B14 coated on a 96-well plate.

Recovery

[ { "sample": "Serum", "range": "110 - 141 %", "average": "= 131.3" }, { "sample": "Plasma", "range": "108 - 144 %", "average": "= 130.8" }, { "sample": "Cell culture media", "range": "89 - 124 %", "average": "= 105.6" } ]

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313972-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"700X HRP-Streptavidin", "number":"AB313972-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313972-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human HSD17B14 Antibody-coated ELISA Plate", "number":"AB313972-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human HSD17B14 Detection Antibody", "number":"AB313972-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313972-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313972-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313972-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human HSD17B14 Protein Standard", "number":"AB313972-CMP10", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Has NAD-dependent 17-beta-hydroxysteroid dehydrogenase activity. Converts oestradiol to oestrone. The physiological substrate is not known. Acts on oestradiol and 5-androstene-3-beta,17-beta-diol (in vitro).
See full target information HSD17B14
websiteProtocolBooklet
en

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