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AB284006

Human HSP27 ELISA Autoantibody Kit

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Human HSP27 ELISA Autoantibody Kit is a Sandwich ELISA for the measurement of Human HSP27 ELISA Autoantibody Kit in Human in Biofluids samples.

View Alternative Names

HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27

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Sandwich ELISA - Human HSP27 ELISA Autoantibody Kit (AB284006)
  • sELISA

Supplier Data

Sandwich ELISA - Human HSP27 ELISA Autoantibody Kit (AB284006)

Example of Human Hsp27 Autoantibody standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.2 AU/mL

Range

1.5 - 24 AU/mL

Assay time

4h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

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Product details

Human HSP27 ELISA Autoantibody Kit (ab284006) is designed for the quantitative determination of autoimmune response (IgG) to a target antigen (HSP27). The kit detects autoantibodies in plasma and serum samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures autoantibodies (anti-HSP27 IgG) in less than 4 hours.

An HSP27 antigen has been pre-coated onto a 96-well microplate with removable strips. Autoantibody specific for HSP27 in standards and samples is sandwiched by the immobilized antigen and an antibody-HRP conjugate. The color development is stopped and the intensity of the color is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab284006-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB284006-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB284006-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Chromogen Substrate", "number":"AB284006-CMP04", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB284006-CMP10", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB284006-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human HSP27 Microplate", "number":"AB284006-CMP03", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB284006-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"HSP27 Standard", "number":"AB284006-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"50X Biotinylated Human Hsp27 Autoantibody Antibody", "number":"AB284006-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Diluent M Concentrate", "number":"AB284006-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.
Biological function summary

Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.

Pathways

Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.

Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.
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Product protocols

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Target data

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed : 10383393, PubMed : 20178975). Plays a role in stress resistance and actin organization (PubMed : 19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed : 23728742).
See full target information HSPB1
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Product promise

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